General information for g14300.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dihydrofolate reductase.
Parent gene
- The parent gene g14300 is located on chr_1: 36418762..36419384 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14300 | g14300.t1 | TTS | g14300.t1 | 36418691 | 36418691 |
| chr_1 | g14300 | g14300.t1 | isoform | g14300.t1 | 36418762 | 36419384 |
| chr_1 | g14300 | g14300.t1 | exon | g14300.t1.exon1 | 36418762 | 36419239 |
| chr_1 | g14300 | g14300.t1 | cds | g14300.t1.CDS1 | 36418762 | 36419239 |
| chr_1 | g14300 | g14300.t1 | exon | g14300.t1.exon2 | 36419299 | 36419384 |
| chr_1 | g14300 | g14300.t1 | cds | g14300.t1.CDS2 | 36419299 | 36419384 |
| chr_1 | g14300 | g14300.t1 | TSS | g14300.t1 | 36419558 | 36419558 |
Sequences
>g14300.t1 Gene=g14300 Length=564
ATGAAAAATCTCAAACTGAATTTGATTGCTGCTGCTTGTGAAAACATGGGAATTGGATAT
AAAGGTGATCTACCTTGGTTTCTTAAGAATGAATTGAAACATTTTTCAAGAACAACAAAA
AAGATTTCAGATCCGTCGAAGAAAAATGTCGTGATAATGGGTCGATTAACTTATTTTGGA
GTTCCTGAAAGTAAAAGACCATTACCAGAAAGAATTAATATCGTTCTAACAACAAATCCA
GACAAATATCAATTTCCATCAGAAGTGATTGTTGCTAAAAGCATGAATGAAGCGCTCGAA
AGACTTCAGGAACCGGAATTAGCTGATAAAATCGAAAATGTTTGGATTGTTGGTGGTAAT
ACAGTGTATAAAGAAGCAATGGAATCATCTCTTTGTCATCGCATTTATTTAACACGCGTT
ATGGCTACTTTCGAATGTGATGCATTTTTTCCTAATTTAACTGAAGATTTTAAAAGAGTC
CCAAATGATGAAGACATAACTGAAGAGATTCAGGAAGAAAATGGTATTAAATACCAGTAT
CAAACATATGAAAAAATTCATTAA
>g14300.t1 Gene=g14300 Length=187
MKNLKLNLIAAACENMGIGYKGDLPWFLKNELKHFSRTTKKISDPSKKNVVIMGRLTYFG
VPESKRPLPERINIVLTTNPDKYQFPSEVIVAKSMNEALERLQEPELADKIENVWIVGGN
TVYKEAMESSLCHRIYLTRVMATFECDAFFPNLTEDFKRVPNDEDITEEIQEENGIKYQY
QTYEKIH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g14300.t1 | CDD | cd00209 | DHFR | 6 | 184 | 1.04574E-61 |
| 8 | g14300.t1 | Gene3D | G3DSA:3.40.430.10 | Dihydrofolate Reductase | 4 | 187 | 2.3E-61 |
| 2 | g14300.t1 | PANTHER | PTHR48069 | DIHYDROFOLATE REDUCTASE | 1 | 186 | 1.2E-59 |
| 6 | g14300.t1 | PRINTS | PR00070 | Dihydrofolate reductase signature | 17 | 27 | 1.9E-13 |
| 5 | g14300.t1 | PRINTS | PR00070 | Dihydrofolate reductase signature | 31 | 39 | 1.9E-13 |
| 4 | g14300.t1 | PRINTS | PR00070 | Dihydrofolate reductase signature | 51 | 62 | 1.9E-13 |
| 3 | g14300.t1 | PRINTS | PR00070 | Dihydrofolate reductase signature | 113 | 127 | 1.9E-13 |
| 1 | g14300.t1 | Pfam | PF00186 | Dihydrofolate reductase | 6 | 185 | 8.9E-37 |
| 10 | g14300.t1 | ProSitePatterns | PS00075 | Dihydrofolate reductase (DHFR) domain signature. | 17 | 39 | - |
| 11 | g14300.t1 | ProSiteProfiles | PS51330 | Dihydrofolate reductase (DHFR) domain profile. | 5 | 185 | 50.528 |
| 7 | g14300.t1 | SUPERFAMILY | SSF53597 | Dihydrofolate reductase-like | 2 | 186 | 2.62E-57 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0046654 | tetrahydrofolate biosynthetic process | BP |
| GO:0055114 | NA | NA |
| GO:0006545 | glycine biosynthetic process | BP |
| GO:0004146 | dihydrofolate reductase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00287 (dihydrofolate reductase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
