General information for g14300.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dihydrofolate reductase.
Parent gene
- The parent gene g14300 is located on chr_1: 36418762..36419384 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14300 | g14300.t2 | isoform | g14300.t2 | 36418086 | 36419384 |
| chr_1 | g14300 | g14300.t2 | exon | g14300.t2.exon1 | 36418086 | 36418233 |
| chr_1 | g14300 | g14300.t2 | exon | g14300.t2.exon2 | 36418357 | 36418430 |
| chr_1 | g14300 | g14300.t2 | exon | g14300.t2.exon3 | 36418597 | 36418650 |
| chr_1 | g14300 | g14300.t2 | cds | g14300.t2.CDS1 | 36418623 | 36418650 |
| chr_1 | g14300 | g14300.t2 | exon | g14300.t2.exon4 | 36418826 | 36419384 |
| chr_1 | g14300 | g14300.t2 | cds | g14300.t2.CDS2 | 36418826 | 36419169 |
| chr_1 | g14300 | g14300.t2 | TSS | g14300.t2 | 36419558 | 36419558 |
| chr_1 | g14300 | g14300.t2 | TTS | g14300.t2 | NA | NA |
Sequences
>g14300.t2 Gene=g14300 Length=835
ATGAAAAATCTCAAACTGAATTTGATTGCTGCTGCTTGTGAAAACATGGGAATTGGATAT
AAAGGTGATCTACCTTGGTTTCTTAAGTAAGTAATTACATTTAAAGATGGTAAATAACTA
ACATTCAAAAACGTTATCCTTTTAGGAATGAATTGAAACATTTTTCAAGAACAACAAAAA
AGATTTCAGATCCGTCGAAGAAAAATGTCGTGATAATGGGTCGATTAACTTATTTTGGAG
TTCCTGAAAGTAAAAGACCATTACCAGAAAGAATTAATATCGTTCTAACAACAAATCCAG
ACAAATATCAATTTCCATCAGAAGTGATTGTTGCTAAAAGCATGAATGAAGCGCTCGAAA
GACTTCAGGAACCGGAATTAGCTGATAAAATCGAAAATGTTTGGATTGTTGGTGGTAATA
CAGTGTATAAAGAAGCAATGGAATCATCTCTTTGTCATCGCATTTATTTAACACGCGTTA
TGGCTACTTTCGAATGTGATGCATTTTTTCCTAATTTAACTGAAGATTTTAAAAGAGTCC
CAAATGATGAAGACATAACCTCTGCTTTTTTTAGCTCTTTTCCTTAACCTTCATCCATTT
TCTTAAAAGGAACATAATAATTTTCCTTACTTTATTTCTTTCTAGAGAAAATTTTTTGTA
TGTATTGCTATGAGGGTAGAAAAAATATCGCGTACAAATTTTAAATCGTTTTTTGTAATA
TCCCCATTTTATATTTTACGCTTTTCGCTCTCACTCTTATGATCTCTCTAGAGAATTGTA
CTCGAAAAACATATTTCTTTATTACGAGTGTGTATAAAATTTTCTATCTTTTACC
>g14300.t2 Gene=g14300 Length=123
MGRLTYFGVPESKRPLPERINIVLTTNPDKYQFPSEVIVAKSMNEALERLQEPELADKIE
NVWIVGGNTVYKEAMESSLCHRIYLTRVMATFECDAFFPNLTEDFKRVPNDEDITSAFFS
SFP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g14300.t2 | CDD | cd00209 | DHFR | 1 | 120 | 0.000 |
| 4 | g14300.t2 | Gene3D | G3DSA:3.40.430.10 | Dihydrofolate Reductase | 1 | 120 | 0.000 |
| 2 | g14300.t2 | PANTHER | PTHR48069 | DIHYDROFOLATE REDUCTASE | 1 | 112 | 0.000 |
| 1 | g14300.t2 | Pfam | PF00186 | Dihydrofolate reductase | 1 | 110 | 0.000 |
| 5 | g14300.t2 | ProSiteProfiles | PS51330 | Dihydrofolate reductase (DHFR) domain profile. | 1 | 123 | 31.643 |
| 3 | g14300.t2 | SUPERFAMILY | SSF53597 | Dihydrofolate reductase-like | 1 | 112 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0046654 | tetrahydrofolate biosynthetic process | BP |
| GO:0055114 | NA | NA |
| GO:0006545 | glycine biosynthetic process | BP |
| GO:0004146 | dihydrofolate reductase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed