General information for g14305.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Developmental protein eyes absent.
Parent gene
- The parent gene g14305 is located on chr_1: 36514569..36532805 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14305 | g14305.t1 | isoform | g14305.t1 | 36514569 | 36532805 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon1 | 36514569 | 36514652 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS1 | 36514569 | 36514652 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon2 | 36522383 | 36522435 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS2 | 36522383 | 36522435 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon3 | 36530029 | 36530594 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS3 | 36530029 | 36530594 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon4 | 36530651 | 36530822 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS4 | 36530651 | 36530822 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon5 | 36530974 | 36531574 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS5 | 36530974 | 36531574 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon6 | 36531632 | 36531827 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS6 | 36531632 | 36531827 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon7 | 36532147 | 36532258 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS7 | 36532147 | 36532258 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon8 | 36532314 | 36532555 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS8 | 36532314 | 36532555 |
| chr_1 | g14305 | g14305.t1 | exon | g14305.t1.exon9 | 36532624 | 36532805 |
| chr_1 | g14305 | g14305.t1 | cds | g14305.t1.CDS9 | 36532624 | 36532805 |
| chr_1 | g14305 | g14305.t1 | TTS | g14305.t1 | 36533659 | 36533659 |
| chr_1 | g14305 | g14305.t1 | TSS | g14305.t1 | NA | NA |
Sequences
>g14305.t1 Gene=g14305 Length=2208
ATGGTGACATTAATGCCTTGTAGTTATTTATCAGCTCCCCGCTGTGCAGGACTGATTGAT
AAAATGATAGAGATTGAACCAAAGGTTAAACGTGCAAAGACCGAACCATCAGAAGCAAGT
AGTGATAAAAATAATCGATTATGTAGTGCAGAGATTTCAACCCAAAGTGATTGTAGCATT
TCGCCAGCTCTTCTTCCTTATAATTTACACCAGAATTCATCAAATCAAACATTGCTGAAT
CATCAATATAATCGACATAATCAGCAGCAGCAACAAAATTCTCCATCATTAATTGGTGGC
TTGAATCTTTCAGCATCATCTTCGTCCACATCGGCAACGTCGCCTACAGGTGCGTCTTCA
TCAGCGCCATCAACTATTCCTACCACTAGCACGTCCATAACAACATTGAGCAATAATCTG
TATAATAACAATCATCTTTATCAACATCATCATCATCATCATCTGAATACAACAACGGGA
AGTAGTGGACATAATTCCAAGCACAATTTGTCTACAAGCAGCGGGACAGCAGCGACAGCA
ATAACACATCATCCTACGCCAATTGAGGGTTTGACGGCTCTATCGTCACTTGGTAGTTCA
ACACTACATTTGCCCACAAATTCTTTGTGCAGTGGAAATTCAAATTCTTTAAGCGCTGAA
CTGGGCATGTCACATTGGCTAAATGATACACCCAGTAATTCTGTGAAATCAGAAATAAAA
AGTCCAACGGCAATTGTCGAAAGTACACAATTGCCACTTGCGCCTTCTCATCTTGATTCA
GCACTATTTTGTGCAGCAAATACTGTTAATTTAGACGCTTCTCAAAGTTCAAATACTTAT
GATCACAAATCAGATTATTACAATTATTATAATAGCATGCAACAATATACGCCATCATTT
TATCCATCCGCATATGGATCAGCTTATACCACGCGATCATCAACAAAAATTCCATCGCCC
AATTCGTATCTATCGTCGAGTTACACCACAAATAATAATTCAGCACAACTCTATTCTACT
TATGGATATAATAATTTTGGACAATTTGCAACGCCACAGCAGGACTATTATTATAATGAC
CAGTACAATAGTTATTATAATGCCAACTATTCACCTTACGTTAGCTCGCCTGGTTCAAGT
AGTAGTCAAAATTTTCATATTGCTGCTATGCCAGAAAGTCCATCCGAAACGCCTGGTACG
CCTTCCGGTTTAGGCCACCATTCACCACAATCGCCTCTGTCAATTTCACCAAATACATCG
AACCATGCATCAACAGCTAAAACAACGCCATCAACTAAAGGCAAGGCGCGTGGCAGAAGA
CAACAGAATACAAGTCCCACGCGATCAGCACTCAACGATACGCCCTCAGTTGAAAATGTC
AAATCACCAGAAAGAGTTTTTATTTGGGATCTTGATGAAACCATTATTATTTTCCATACA
CTGCTTACTGGCAATTACCCAGCTCGATATAATAAGGATCCAGCTTTAGCAACAACCCTT
GGCTATAGAATGGAAGAAATGATTTTTACAATGGCTGATAATCATTTCTTTTTTAATGAT
GTTGAAGAATGTGATCAAGTGCATATTGATGATGTTTCATCTGACGATAACGGACAAGAT
TTGAGCAATTATAATTTCGCTACTGATGGCTTTCAAAATAATAATAGTCAAGGAGCGACA
AATATTTGCCTGCCAAATGGCGTTCGTGGCGGTGTCGATTGGATGAGAAAACTTGCATTT
CGTTATCGTAAAATTAAGGAAATTTATAATAATTATAGAAATAATGTTGGTGGCCTATTG
GGACCAAAATGTGAGCATTGGATGCAAGTAAGAAGTGAAATTGAGGCATTGACAGATAAC
TGGGCTACACTTGTCACAACATGCCTCAAAATGATTGCTCAACGCGAAAATTGTATAAAT
GTTCTTGTCACAACGACACAATTGGTACCGGCATTAGCGAAAGTTTTATTGTTTGGCTTG
GGTGGTGTCTTTCCCATAGAAAATATTTACAGTGCAACCAAAACTGGCAAAGAGTCATGT
TTCGAACGTATTGTAACGAGATTTGGACGAAAATGCACATATGTTGTCGTTGGCGATGGA
CAGGATGAAGAAAAGGCATCGAAGAATCTCAATTTTCCATTCTGGCGAATATCAACGCAT
AAAGACATTAAAGCATTGTACACGGCACTTGAAATGGGCTTCTTATGA
>g14305.t1 Gene=g14305 Length=735
MVTLMPCSYLSAPRCAGLIDKMIEIEPKVKRAKTEPSEASSDKNNRLCSAEISTQSDCSI
SPALLPYNLHQNSSNQTLLNHQYNRHNQQQQQNSPSLIGGLNLSASSSSTSATSPTGASS
SAPSTIPTTSTSITTLSNNLYNNNHLYQHHHHHHLNTTTGSSGHNSKHNLSTSSGTAATA
ITHHPTPIEGLTALSSLGSSTLHLPTNSLCSGNSNSLSAELGMSHWLNDTPSNSVKSEIK
SPTAIVESTQLPLAPSHLDSALFCAANTVNLDASQSSNTYDHKSDYYNYYNSMQQYTPSF
YPSAYGSAYTTRSSTKIPSPNSYLSSSYTTNNNSAQLYSTYGYNNFGQFATPQQDYYYND
QYNSYYNANYSPYVSSPGSSSSQNFHIAAMPESPSETPGTPSGLGHHSPQSPLSISPNTS
NHASTAKTTPSTKGKARGRRQQNTSPTRSALNDTPSVENVKSPERVFIWDLDETIIIFHT
LLTGNYPARYNKDPALATTLGYRMEEMIFTMADNHFFFNDVEECDQVHIDDVSSDDNGQD
LSNYNFATDGFQNNNSQGATNICLPNGVRGGVDWMRKLAFRYRKIKEIYNNYRNNVGGLL
GPKCEHWMQVRSEIEALTDNWATLVTTCLKMIAQRENCINVLVTTTQLVPALAKVLLFGL
GGVFPIENIYSATKTGKESCFERIVTRFGRKCTYVVVGDGQDEEKASKNLNFPFWRISTH
KDIKALYTALEMGFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g14305.t1 | CDD | cd02601 | HAD_Eya | 463 | 735 | 1.05133E-155 |
| 6 | g14305.t1 | Gene3D | G3DSA:3.40.50.12350 | - | 464 | 735 | 1.5E-132 |
| 3 | g14305.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 104 | 129 | - |
| 5 | g14305.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 151 | 174 | - |
| 4 | g14305.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 158 | 174 | - |
| 2 | g14305.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 390 | 457 | - |
| 1 | g14305.t1 | PANTHER | PTHR10190 | EYES ABSENT | 272 | 735 | 5.9E-149 |
| 9 | g14305.t1 | SFLD | SFLDG01129 | C1.5: HAD, Beta-PGM, Phosphatase Like | 464 | 731 | 2.2E-31 |
| 10 | g14305.t1 | SFLD | SFLDS00003 | Haloacid Dehalogenase | 464 | 731 | 2.2E-31 |
| 7 | g14305.t1 | TIGRFAM | TIGR01658 | EYA-cons_domain: EYA conserved domain | 463 | 735 | 1.1E-105 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007275 | multicellular organism development | BP |
| GO:0004725 | protein tyrosine phosphatase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K15616 (eyes absent homolog 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed