General information for g14391.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dihydroorotate dehydrogenase (quinone), mitochondrial.
Parent gene
- The parent gene g14391 is located on chr_4: 247362..248632 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14391 | g14391.t1 | TTS | g14391.t1 | 247228 | 247228 |
| chr_4 | g14391 | g14391.t1 | isoform | g14391.t1 | 247362 | 248632 |
| chr_4 | g14391 | g14391.t1 | exon | g14391.t1.exon1 | 247362 | 248333 |
| chr_4 | g14391 | g14391.t1 | cds | g14391.t1.CDS1 | 247362 | 248333 |
| chr_4 | g14391 | g14391.t1 | exon | g14391.t1.exon2 | 248402 | 248632 |
| chr_4 | g14391 | g14391.t1 | cds | g14391.t1.CDS2 | 248402 | 248632 |
| chr_4 | g14391 | g14391.t1 | TSS | g14391.t1 | 248711 | 248711 |
Sequences
>g14391.t1 Gene=g14391 Length=1203
ATGAGCTCATCAAAATTCTTTCGTCGTCTTCGTTCACTTATTGTTATTTCTACGGCTGCA
ACTGGAATCACATTTGGATTTTCTTATTATCGCAATGATGAGCAGTTTTTTAAAACAATT
TTTATGCCGACTGTGAGATTATTGGAAGCAGAAAGAGCTCATGAACTAGCAGTTTTTATT
TGTAAGTGGAATTTTCTTCCATCAGTCAATTACAAAGATCCAGCATCTCTAAGTACTGAG
ATTTGTAACATTGCATTAAAAAATCCAGTCGGTATTGCAGCCGGTTTTGACAAAAACGCA
GAAGCAATTGAAGGTCTCGATCATCTTGGTTTTGGATTTATTGAAGTTGGGAGTGTAACA
CCATTGGCTCAAGTCGGTAATCAAAAGCCAAGAGTTTTTCGTCTTACTGAAGATGAAGCG
ATTATAAATCGTTATGGATTTAATAGTGATGGACATGAAAAAGTTTGGCATCGAATTCAA
CAATTGAGAGCGAGTGGAAAATTTAATGGCGTTCTTGGAGTAAATTTGGGAAAAAATAAA
ATGTCTGAGGATGCAGTTAATGATTATCTTGAAGGTTTAAAGTTATTTTCACCTGTTGCT
GATTATTTAGTCATAAATGTGAGCAGTCCTAATACTCCAGGTTTGAGAGATTTGCAGCAA
AAAGATAGCTTAAAGACATTGTTGTGTAAAATAAATGAAACAAAAAAGCAAATTGAAACT
CCAAAACCAATTTTTGTTAAGCTTGCTCCAGACCTTACACAGTCAGAACTTAAAGATATT
TGTAATACAATTCAGCGCAAAGATTGTCATGTTGATGGTTTAATTATTTCAAACACAACA
ATTGATCGATCTGTGGCACTTAACAGTAGCAACGCATTTGAAGTTGGTGGACTAAGTGGT
AAACCATTGAAAGAAAAAAGTCGAAGAATGATTGAAGATGTTTATAAAATGACAAATGGA
AAAATCCCGATAATTGGGGTTGGTGGTATTGGCAGTGGTCAAGATGCTTATGAAAAAATC
ATTGCTGGTGCAAGTGCAATTCAACTTTATTCAAGTTTTGTTTTTCACGGACCACCATTG
GTTACAAAAGTCAAAAAAGAACTTGATGACATTTTAAAATCAAATGGTTATCAAAATGTT
CAACAGGCTGTCGGCAAAGGAGTTCAAATGGATAAAAAATCTTTCTTATCTTACTTTTTT
TAA
>g14391.t1 Gene=g14391 Length=400
MSSSKFFRRLRSLIVISTAATGITFGFSYYRNDEQFFKTIFMPTVRLLEAERAHELAVFI
CKWNFLPSVNYKDPASLSTEICNIALKNPVGIAAGFDKNAEAIEGLDHLGFGFIEVGSVT
PLAQVGNQKPRVFRLTEDEAIINRYGFNSDGHEKVWHRIQQLRASGKFNGVLGVNLGKNK
MSEDAVNDYLEGLKLFSPVADYLVINVSSPNTPGLRDLQQKDSLKTLLCKINETKKQIET
PKPIFVKLAPDLTQSELKDICNTIQRKDCHVDGLIISNTTIDRSVALNSSNAFEVGGLSG
KPLKEKSRRMIEDVYKMTNGKIPIIGVGGIGSGQDAYEKIIAGASAIQLYSSFVFHGPPL
VTKVKKELDDILKSNGYQNVQQAVGKGVQMDKKSFLSYFF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g14391.t1 | CDD | cd04738 | DHOD_2_like | 42 | 368 | 3.55895E-165 |
| 6 | g14391.t1 | Gene3D | G3DSA:3.20.20.70 | Aldolase class I | 30 | 390 | 1.7E-141 |
| 2 | g14391.t1 | PANTHER | PTHR48109 | DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED | 18 | 386 | 4.1E-127 |
| 3 | g14391.t1 | PANTHER | PTHR48109:SF4 | DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL | 18 | 386 | 4.1E-127 |
| 1 | g14391.t1 | Pfam | PF01180 | Dihydroorotate dehydrogenase | 77 | 372 | 9.4E-101 |
| 7 | g14391.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 9 | g14391.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 30 | - |
| 8 | g14391.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 31 | 400 | - |
| 13 | g14391.t1 | ProSitePatterns | PS00911 | Dihydroorotate dehydrogenase signature 1. | 112 | 131 | - |
| 12 | g14391.t1 | ProSitePatterns | PS00912 | Dihydroorotate dehydrogenase signature 2. | 324 | 344 | - |
| 4 | g14391.t1 | SUPERFAMILY | SSF51395 | FMN-linked oxidoreductases | 37 | 387 | 7.94E-103 |
| 5 | g14391.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 19 | - |
| 14 | g14391.t1 | TIGRFAM | TIGR01036 | pyrD_sub2: dihydroorotate dehydrogenase (fumarate) | 45 | 386 | 8.1E-123 |
| 11 | g14391.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 13 | 30 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016627 | oxidoreductase activity, acting on the CH-CH group of donors | MF |
| GO:0016020 | membrane | CC |
| GO:0004152 | dihydroorotate dehydrogenase activity | MF |
| GO:0006207 | ‘de novo’ pyrimidine nucleobase biosynthetic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0055114 | NA | NA |
| GO:0005737 | cytoplasm | CC |
KEGG
Orthology
- This transcript has been annotated with K00254 (dihydroorotate dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
