General information for g14398.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphoenolpyruvate carboxykinase [GTP].
Parent gene
- The parent gene g14398 is located on chr_4: 268472..269449 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14398 | g14398.t7 | TTS | g14398.t7 | 266020 | 266020 |
| chr_4 | g14398 | g14398.t7 | isoform | g14398.t7 | 266046 | 268949 |
| chr_4 | g14398 | g14398.t7 | exon | g14398.t7.exon1 | 266046 | 266525 |
| chr_4 | g14398 | g14398.t7 | cds | g14398.t7.CDS1 | 266046 | 266525 |
| chr_4 | g14398 | g14398.t7 | exon | g14398.t7.exon2 | 266585 | 266759 |
| chr_4 | g14398 | g14398.t7 | cds | g14398.t7.CDS2 | 266585 | 266759 |
| chr_4 | g14398 | g14398.t7 | exon | g14398.t7.exon3 | 266817 | 268165 |
| chr_4 | g14398 | g14398.t7 | cds | g14398.t7.CDS3 | 266817 | 267091 |
| chr_4 | g14398 | g14398.t7 | exon | g14398.t7.exon4 | 268918 | 268949 |
| chr_4 | g14398 | g14398.t7 | TSS | g14398.t7 | 269106 | 269106 |
Sequences
>g14398.t7 Gene=g14398 Length=2036
ATGTTTCGTGTTTTAAAAAGAGTTAGTACAATATCTTTCAATCATCATTCAACCATTAAT
GCTTCGTTTTTGAATCGTAAAATTCATTCCTATCAACTTGCAAATGGATTCGATCCAGCC
GTACTTCCATCTATGGTTCGAAATTTCATTGAAGATTCTGCTCGACTCTGTGAACCTGAT
TCAATACACATTTGTGATGGAAGTGAAAGTGAAAATAAAATGATGCTAAATGAAATGCAA
GCTTCTGGTACAATCGTATCATTACCAAAATACGATAATTGTTGGTTAGCTAAAACAAAT
CCAGCTGATGTTGCTCGTGTTGAAAGTAAAACTTTTATTTGTACTGAAATGAAAGAAGAT
GCTGTTTGTATACCAAAAAAAGGAGTAACTGGTATGCTCGGAAATTGGATTAGTCCTCAA
GATTACGAAAAAGCTATTTTAGACAGATTTCCAGGTTATTTCGTAGAAATTTACTGAATG
AAATATTTTAAAATAAAAAACTTTATAGGTTCGATGAGAGGCAGAAAAATGTATGTTGTA
CCATTTTCAATGGGTCCATTAGGTTCAAAACTATCAAAAATTGGAATAGAAATAACGGAC
TCCCCATATGTCGTCGCATCAATGCGTATTATGACTCGAATGGGTTCATCCGTTTTAGAC
TTAATTGATGATGATTTTGTTCGTTGTCTTCATTCTATTGGGCGTCCTGCAAGTGGAATC
ATTGAAATGCCTTCATGGATTTGTGATCCACAAAGAACGATCATTTTGCATAAAGCAGAC
AAAAATGAAATTGTCTCGTATGGTTCTGCCTATGGTGGAAATGCACTTTTGGGCAAAAAA
TGTTTTGCTCTTCGTATTGGAAGTAAAATTGCACAAAGAGAAGGCTGGTTGGCTGAGCAT
ATGTTGATTCTTGGTATAACAGCACCTAATGGTATAAAAAGATATATTGCTGCTGCTTTT
CCATCTGCTTGCGGTAAGACAAATTTAGCTATGATGATGCCGACATTAAAAGGATTTAAG
GTTGAAAAGTTTTTTAAATAAATTTTCATTTGGTTCACTTAAACAATTATTTCAAGGTTG
AATGCGTGGGTGATGATATTGCATGGATGAAATTTGATAAAAATGGCCAATTGCGCGCAA
TTAATCCAGAAAATGGATTTTTTGGTGTTGCTCCTGGCACATCATACGAAACAAATCCAA
ATGCAATGAAAACATGTTTAAAAGATACTATTTTTACAAATGTTGCTTCTACTTCTGATG
GGGGTGTTTATTGGGAAGGACTTGAAGATTCAATTGGTACAAATATTTCAATTACTGATT
GGCATGGGAAACCTTGGAAAAGAGGTGTCAATAATACTACATCTGCACATCCGAATTCAC
GATTTTGTGCACCAGCAAAAAATTGTCCAATAATGGATTCTAAATGGGAAGATCCTGAAG
GTGTGCCCATTTCAGCAATTCTTTTCGGTGGACGACGACCTGAAGGTGTTCCACTTGTTT
ATGAAGCTTACAATTGGCAGCATGGCGTGATGATCGGGTCCGCTATGCGATCAGAGGCAA
CAGCAGCAGCTGAACATAAGGGAAAAGTTATTATGCATGATCCAATGGCAATGCGTCCTT
TTCTAGGTTATAATTTTGGAAAATATTTACAACATTGGTTAAATATGGAAGTTGCTGGTT
GTAAAATGCCTAAAATTTTTCATGTGAATTGGTTCCTTAAAGGAAATGATGGAAAGTTTT
TATGGCCAGGATATGGTGAAAATTCACGAGTTCTTGATTGGATTTTAAAGCGAATTGAAG
GTGAAGAGTGTTTTGATGAAACTTCAATTGGAAAAGTTCCATGCAGTAATTCAATTCGAT
TAGATGGATTAAAACAAGAAGTTGACATGAAAAAATTATTTAAAATTGATAAGGAGTTTT
GGTTAAAAGAAGTGAAAGAAATTCAAAAGTTTTATGAAGAACAAGTTGGAAATGATTTGC
CAGAGGCAGTGAAAAATGAATTGAATGGATTGAAGGAAAGAGTCGAACAAATGTAA
>g14398.t7 Gene=g14398 Length=309
MKFDKNGQLRAINPENGFFGVAPGTSYETNPNAMKTCLKDTIFTNVASTSDGGVYWEGLE
DSIGTNISITDWHGKPWKRGVNNTTSAHPNSRFCAPAKNCPIMDSKWEDPEGVPISAILF
GGRRPEGVPLVYEAYNWQHGVMIGSAMRSEATAAAEHKGKVIMHDPMAMRPFLGYNFGKY
LQHWLNMEVAGCKMPKIFHVNWFLKGNDGKFLWPGYGENSRVLDWILKRIEGEECFDETS
IGKVPCSNSIRLDGLKQEVDMKKLFKIDKEFWLKEVKEIQKFYEEQVGNDLPEAVKNELN
GLKERVEQM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g14398.t7 | Gene3D | G3DSA:3.90.228.20 | - | 51 | 307 | 0 |
| 2 | g14398.t7 | PANTHER | PTHR11561 | PHOSPHOENOLPYRUVATE CARBOXYKINASE | 1 | 309 | 0 |
| 3 | g14398.t7 | PANTHER | PTHR11561:SF11 | PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP], MITOCHONDRIAL | 1 | 309 | 0 |
| 1 | g14398.t7 | Pfam | PF00821 | Phosphoenolpyruvate carboxykinase C-terminal P-loop domain | 1 | 306 | 0 |
| 4 | g14398.t7 | SUPERFAMILY | SSF53795 | PEP carboxykinase-like | 1 | 308 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006094 | gluconeogenesis | BP |
| GO:0005525 | GTP binding | MF |
| GO:0004611 | phosphoenolpyruvate carboxykinase activity | MF |
| GO:0017076 | purine nucleotide binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
