General information for g14417.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heat shock protein 75 kDa, mitochondrial.
Parent gene
- The parent gene g14417 is located on chr_4: 405396..408513 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14417 | g14417.t1 | TSS | g14417.t1 | 405307 | 405307 |
| chr_4 | g14417 | g14417.t1 | isoform | g14417.t1 | 405396 | 407765 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon1 | 405396 | 405477 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS1 | 405396 | 405477 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon2 | 405539 | 405988 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS2 | 405539 | 405988 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon3 | 406050 | 406480 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS3 | 406050 | 406480 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon4 | 406541 | 407146 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS4 | 406541 | 407146 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon5 | 407207 | 407627 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS5 | 407207 | 407627 |
| chr_4 | g14417 | g14417.t1 | exon | g14417.t1.exon6 | 407698 | 407765 |
| chr_4 | g14417 | g14417.t1 | cds | g14417.t1.CDS6 | 407698 | 407765 |
| chr_4 | g14417 | g14417.t1 | TTS | g14417.t1 | 408375 | 408375 |
Sequences
>g14417.t1 Gene=g14417 Length=2058
ATGAGTCTAAAAATTGCAACAAGAAGTGTACTAAGATGCAAAAGTAGTTTTATGTCATTT
AGTAAAACCTGGAATTATCGAGTTAATAAGTCAATAATACCAGCACTTACTCAAACGACA
ATTGCTCATTATCATTCAATAATTCGTGAATCAGAAAAAGTTAAAGGTGAAGCTGATAGA
CATGAGTTTAAAGCAGAAACAAGGATGTTACTGGACATTGTTGCTAAATCTTTATATTCA
GAAAATGAAGTTTTTGTCCGAGAGTTAATATCAAATGCAAGTGATGCTATTGAAAAATTT
CGATATATTTCAAGTACAAAACCTGATGATGTAATTGATTTGGATCGAAAACTTGAAATC
CATATTGAAACAAATAAACAAAATAGAACACTCATCATACAAGACACTGGTATTGGAATG
ACCAAAGAAGAAATGATTGATGCACTTGGTACTATTGCCAGAAGTGGTTCAAAAAACTTT
CTACAACAGATTCAAAAAGGAAAAGAACAAGGAGCTGCTGCTAATATTATTGGACAATTT
GGTGTTGGTTTTTATTCAGCATTTATGTGTGCCACTCATGTCGAAGTCTATTCAAGAGCA
TCAAATAACACTGATAGTGTTGGATATAAATGGTCAAGTGATGGCACTGGATCATTTGAA
ATTCAAGAATGTGATGATGTTGCAGTTGGAACTAAAATTGTAGTTCATTTAAAACCTGAA
TGTCGTGAATATGCAGAAGAAAATCGAATTCATGATGTAATTAAAAAATACAGCAATTTT
GTTGGTTCGCCAATTTTTCTTAATGGAAATCAAGTCAATTTAGTTCACCCATTATGGCTT
ATGGAACCAAAAGATGTTATAGATGAACAACATTCAGAATTCTTTAGATATATCGCAAAT
TCTTTTGATGTTCCACGATATAAGTTACATTTCAAAACCGATGTTCCACTCAGTTTACGG
ACTGTATTGTATTTTCCAGAAGGAAAACCTGGTCTTTTTGAAATGAGTAGAGATGCCGAT
ACAGGTATTGCTTTATATACTCGCAAAATTCTCATTCAATCAAAGACTGACTTGATGCTT
CCAAAATGGCTTAGATTTGTGAAAGGTGTTGTAGATAGTGAAGACATTCCACTCAATCTT
AGTCGTGAATTACTTCAAAACAGTAATTTGATAAGAAGACTAAGAAATGTTCTTACATCG
CGAGTCCTCAAATTTCTGTATGATAAATCAGTAAAAGATCCAGAAAATTATGAAAAATTC
TACAAAGATTATGGATTATTTCTTAAAGAAGGAATTGTAACAACACAAGATCAGCCAGAA
AAAGAAGATATTGCAAAATTGTTGAGATTTGAAACAAACAAAATAAAAGAAGGCACAAGA
ATAGGAATCGATGATTATATTAAGCAGTTGAAAGAAGGCCAGAAAGATATTTACTATTTG
GCAGCTCCATCAAGGGAATTGGCATTAAATTCTCCATATTTTGAAAGTTTACAAAAACAA
GATTATCCAGTACTTTTTTGCTATGAACCATACGATGAACTTGTATTAATGCAATTAATT
AGTTATAAGGGTCATAGTCTCGTAAGTGTTGAAAAAGAAGTTCGCAGGGCAACAACAGTT
GAAGAAAATGACAAAATATTTGATGAAACTTCGAAGGAAGCACTTTCGAGACGTGAGACT
GATGAACTTGTAAGATTTTTAAAAAAAGAGCTTGGACATAAAGTAGCAAATGTGAAATAC
ACAAACAAACTTGACCATCATCCATGTATTGTAACAGTTGAAGATATGGCAGCAGCTCGG
CATTTTATTAAAACGCAAAGTCATCAATTGAGTGAAGATAATCGTTATGCATTGCTTCAA
TCACATTTAGAAATCAATCCAAGACATCCAATTATCAGAAAATTATCAAAATTATCGACG
ACTGATGAAAAACTTGCATCATTATTAGCTCATCAACTTTTTAGTAATGCTATGACAACT
GCTGGTCTTGTCGATGATCCACGGTTACTGTTAACACAAATGAATGAACTTCTAATTAAA
GTTCTTGAAAAGTTTTAA
>g14417.t1 Gene=g14417 Length=685
MSLKIATRSVLRCKSSFMSFSKTWNYRVNKSIIPALTQTTIAHYHSIIRESEKVKGEADR
HEFKAETRMLLDIVAKSLYSENEVFVRELISNASDAIEKFRYISSTKPDDVIDLDRKLEI
HIETNKQNRTLIIQDTGIGMTKEEMIDALGTIARSGSKNFLQQIQKGKEQGAAANIIGQF
GVGFYSAFMCATHVEVYSRASNNTDSVGYKWSSDGTGSFEIQECDDVAVGTKIVVHLKPE
CREYAEENRIHDVIKKYSNFVGSPIFLNGNQVNLVHPLWLMEPKDVIDEQHSEFFRYIAN
SFDVPRYKLHFKTDVPLSLRTVLYFPEGKPGLFEMSRDADTGIALYTRKILIQSKTDLML
PKWLRFVKGVVDSEDIPLNLSRELLQNSNLIRRLRNVLTSRVLKFLYDKSVKDPENYEKF
YKDYGLFLKEGIVTTQDQPEKEDIAKLLRFETNKIKEGTRIGIDDYIKQLKEGQKDIYYL
AAPSRELALNSPYFESLQKQDYPVLFCYEPYDELVLMQLISYKGHSLVSVEKEVRRATTV
EENDKIFDETSKEALSRRETDELVRFLKKELGHKVANVKYTNKLDHHPCIVTVEDMAAAR
HFIKTQSHQLSEDNRYALLQSHLEINPRHPIIRKLSKLSTTDEKLASLLAHQLFSNAMTT
AGLVDDPRLLLTQMNELLIKVLEKF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g14417.t1 | CDD | cd16927 | HATPase_Hsp90-like | 70 | 261 | 3.01018E-91 |
| 19 | g14417.t1 | Gene3D | G3DSA:3.30.565.10 | - | 46 | 275 | 6.6E-75 |
| 20 | g14417.t1 | Gene3D | G3DSA:3.30.230.80 | - | 276 | 448 | 6.9E-62 |
| 17 | g14417.t1 | Gene3D | G3DSA:3.40.50.11260 | - | 449 | 545 | 1.7E-28 |
| 18 | g14417.t1 | Gene3D | G3DSA:1.20.120.790 | - | 548 | 685 | 3.3E-42 |
| 5 | g14417.t1 | Hamap | MF_00505 | Chaperone protein HtpG [htpG]. | 56 | 679 | 23.387127 |
| 3 | g14417.t1 | PANTHER | PTHR11528:SF44 | HEAT SHOCK PROTEIN 75 KDA, MITOCHONDRIAL-RELATED | 30 | 680 | 4.7E-274 |
| 4 | g14417.t1 | PANTHER | PTHR11528 | HEAT SHOCK PROTEIN 90 FAMILY MEMBER | 30 | 680 | 4.7E-274 |
| 21 | g14417.t1 | PIRSF | PIRSF002583 | HSP90_HTPG | 21 | 685 | 7.9E-209 |
| 6 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 59 | 79 | 5.3E-54 |
| 8 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 80 | 102 | 5.3E-54 |
| 7 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 130 | 147 | 5.3E-54 |
| 12 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 148 | 165 | 5.3E-54 |
| 11 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 177 | 199 | 5.3E-54 |
| 9 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 229 | 246 | 5.3E-54 |
| 10 | g14417.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 247 | 265 | 5.3E-54 |
| 1 | g14417.t1 | Pfam | PF13589 | Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase | 82 | 206 | 6.1E-12 |
| 2 | g14417.t1 | Pfam | PF00183 | Hsp90 protein | 270 | 682 | 1.1E-98 |
| 23 | g14417.t1 | SMART | SM00387 | HKATPase_4 | 81 | 241 | 9.2E-4 |
| 14 | g14417.t1 | SUPERFAMILY | SSF55874 | ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase | 60 | 273 | 9.56E-59 |
| 13 | g14417.t1 | SUPERFAMILY | SSF54211 | Ribosomal protein S5 domain 2-like | 276 | 533 | 1.67E-71 |
| 15 | g14417.t1 | SUPERFAMILY | SSF110942 | HSP90 C-terminal domain | 562 | 679 | 2.09E-24 |
| 16 | g14417.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 11 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006457 | protein folding | BP |
| GO:0005524 | ATP binding | MF |
| GO:0051082 | unfolded protein binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09488 (TNF receptor-associated protein 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
