General information for g14435.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Intraflagellar transport protein 122-like protein.
Parent gene
- The parent gene g14435 is located on chr_4: 515635..519292 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14435 | g14435.t1 | TTS | g14435.t1 | 514857 | 514857 |
| chr_4 | g14435 | g14435.t1 | isoform | g14435.t1 | 515635 | 519292 |
| chr_4 | g14435 | g14435.t1 | exon | g14435.t1.exon1 | 515635 | 518990 |
| chr_4 | g14435 | g14435.t1 | cds | g14435.t1.CDS1 | 515635 | 518990 |
| chr_4 | g14435 | g14435.t1 | exon | g14435.t1.exon2 | 519055 | 519164 |
| chr_4 | g14435 | g14435.t1 | cds | g14435.t1.CDS2 | 519055 | 519164 |
| chr_4 | g14435 | g14435.t1 | exon | g14435.t1.exon3 | 519228 | 519292 |
| chr_4 | g14435 | g14435.t1 | cds | g14435.t1.CDS3 | 519228 | 519292 |
| chr_4 | g14435 | g14435.t1 | TSS | g14435.t1 | NA | NA |
Sequences
>g14435.t1 Gene=g14435 Length=3531
ATGCGTGCAATACCAAAATGGGTTGAGAAAATTCATGAACAAGGTGATAAAAGTGATAAT
GCAAGCATTCATGCAGTTTGTTTTCATCCAGAAGCTTATAATCTACTTGTCGCAGCAGGC
GATAAAGTACTCGTATATGATGCAACAGATGGAAATTTAATTGATACTTTAAAAGGTCAT
AAGGACACAGTTTATTGTTTAGCATACAGCCGCGATGGTAAGAAATTTGCAAGTGGTTCA
GCTGATAAGACTGTAATAATTTGGACACATAAGCTTGAAGGAATTTTAAAATATTCTCAC
AATGATGGTATACAATGTCTTGAATTCAATCCAGTTACACACCAACTAGCAAGTTGTACA
ATTTCTGATTTTGCATATTGGTCATCTGAACAAAAAAATGTTCAAAAATATAAAATTCAA
ACGAGAATCAATTGTTGTTCATGGACAATTGATGGACAATATATTGCGCTTGGTCTTGCA
AATGGTCTCGTTTCAATAAGAAATAAATTCGGAGAAGAAAAGATGAAAATTGATCGAGGA
AAAGCACCAATCTATGGATTACAATGGGCACCGCCATCATCAACAAGTGGTCCAGTTGAT
ATTCTTGCAATTGCTGATTGGAATCAAAAATTATCATTTTATACTATAAGTGGTCAAATA
ATTGGTAAAGAACGACAACTAGGGTTTGATCCTTTATGTTTGAGTTTTTTTGCTGATGGA
GAATCATTAGTTGTCAGTGGTTGCAATAAACAACTACAATTGTATACTCGTGATGGAATT
CGTATTGGTCCTCTAGGAGAAATTCATGATTCATGGATCTGGAGTGCTTCAGTAAATCCG
CAAGGAACTGCCGTTGTTGTTGGTTGTCAAGATGGATCACTTGCTTATTATAATCTTACA
TTTAGCACAGTGCATGCACTTTATGCCTCACGTTACGCTTATAGAGAAAATATGTGTGAT
GTCATCATACAACATCTTTTATCGGGACAAAAAGTTCGCATTAAATGTCGTGATTTGGTG
CATAAAATTGCAATTTATAGAACAAAGTTAGCTGTACAGTTACCAGAGCGCGTTGTTCTT
TATGAATTAACAGCAGAAAATCAACCAATGCATTATAAAGTGAAAGAAAAAATTTCTAAA
AAATTCAATTGCTCTTTGCTTGTTGTTTGTTCACAAAATTTAGTACTTTGTCAAGAGAAG
CGATTACAAAGTCTTGATTTTTATGGTGACTTACAGCGTGAGTGGATAATGGACTCATTT
ATACGTTATATTAAAGTCACAGGAGGCTCACCTGGTTCAGAAGGACTCATCATTGGCCTC
AAGAGTGGACAAGTTTGGAGAATTTTCTTAGACAATTCATTGCCAATTCTTTTGACAACA
GTTCTTTCATCAGTTCGCTGTTTGGATTTAAATGCGACTAGAACAAAACTTGCTGTTGTT
GATGATATTGGTAGAATGACTGTTAGAGATTTGACAACCGATACTTTACTCTATCAGGAT
TCAGGTGTAAATTCACTAGCATGGAATACAAATCATGAATCAATGCTTTGCTATTCTCAT
ACAAATGGTGGATTAAGTATCAGAGTAGGATCTTTGCCAGCGAAAAATCCTCAAAATATG
ATTGGAGTCGTTGTTGGATTATGTGGAGCTACAGCATTTTGTTTACGTGGTAATTTAATG
ACAAATATTCCATTGGCTTTAAGTGCAACACTTTGGCAATTTGTCGAAGCAGGACTTTAT
GAGGACGCTGTTGAGATAGCAAATTTAGGCGTTCCACTTAATGATTGGGAAGCATTAGGA
TTTGCAGCACTTGATGCATTAAATTTTAAAGCAGCTCAAGATGCATTTGTAAAAACAAGA
AATTTACCATGGCTTGAATTCATAAACGAAATTTTAACACGTCAAAAACATATTGATATT
TCAAAGGAACTTATTCAAGCTGAAATTTTAGCATTCAGTGGTAAATTTAAAGAAGCAGCA
AGATTATTTCAAAAATGTGGTAAAAATAACAAAGCAATTACGATGTATTGTGATTTAAAA
ATGTTTGATTTAGCTCAACAATTTTTAACTAATAATGAGGACGGTGATGATGTAAATAAT
AGCGATAGCGATGATCGTCGTGAATTGGTCAAGAAAAAAGCTGAATGGGCATGCTCAGTT
CATGAACCAAGAGCAGCTGCAGAATTACTTCTTTCTGCTGGTGAAGTTGAGAGAGCTATA
GAAATTGTTGCTGAACAAGGTTGGACTGATTTGTTACTTGATATTGGACGTAAGTTGCAG
CCAACTGAAAAGAAATCTTTAGAATTGATTGCTAATCATTTAAAAGAATTGAAAGCTTTA
TCAATGGCAGCTGAAATTTATAGAAAATTAGGTGAAGAAACTCAAGTTGTTCAGTTGCAT
ATTGAAGCACGCGATTGGAATGAAGCTTTTAGACTTGCCGAACATTTACCACAAGTGTTG
CCAACAGTACATCTTTCATATGCAAAATGGCTTGTTGATTCAGATCAATTTATTGAAGCA
CATGAAGCATTTGTAGCTGCAAATAAACCCAAAGAAGCTCACATTTTATTAAAAAATTTG
GCAGATTCGGCAGTTACGGAAGAAAGATTTTTAGATGCAAGTTATTATACATGGTTAAGA
GCAAGACAATTGATGAAAATGATGGAAATGAATCCTGAAGAAACATCACAGCATCATATT
AAAGAATTTAAAGTTTTACTACAATTGTCAGCTATTTATTATGCATATGCAACAATTTTT
TCATATTTAAGAGAACCATTTACTTCATCACCACCATTGACACTATTTAATACAAGTCGT
TTTATTGCAAATCAAATTGGTAACAGCACGCCACCAAAAGGCATTTCAATGTTTAATGTT
TATTATACACTTTCAAAACAAGCAAAAGTCCTGGGTGCAAATAAACTTCACTTTCAAATT
AATAACAAACTACAACAATTAAAAGCACCAAATGGAATTCAAGAACAAGTTGATGTAAAC
ATTATGAATTCGCGTGGAGCATCTACTTTTAGTGACCCTGAAGACTTGTTACCAATGTGC
TATAAATGTTCAAACTTTAGCATGCATTTAAGCGGTAATAATTGTCCAAATTGTCAACAA
GAATTTATTTTTTCTTTTGTATCTTTTGAAATTTTACCTTTGGCTCAATTTTTCCCTGAA
TCCGATATAAATGAGGAGGAAGCTGAACGATTATTGATGACACCGCCAAAGGAAAATTCT
GTTGATCCATTTACTGAAACTATCACACAAGATGATATTGGTGATCTATTGCCTATCACA
CTAAATCGTGATGCACTTCGAAGTATTGATCCAAGAAATGTTATTCTCGTTAAAACACCA
AAACCAATTGCAACAAAATATTATCGAAATTTGTTACCAGAATTACAGATTACAATTTGT
CCTGGCTGTTATCAAGCATTTCATTCTGAAGATTTTGAATTACAAGTTCTACAAAAAGGT
TCATGTCCTTTTTGTCGATTGCCAGAGGATCAACTTTTTGATTGCTATTGA
>g14435.t1 Gene=g14435 Length=1176
MRAIPKWVEKIHEQGDKSDNASIHAVCFHPEAYNLLVAAGDKVLVYDATDGNLIDTLKGH
KDTVYCLAYSRDGKKFASGSADKTVIIWTHKLEGILKYSHNDGIQCLEFNPVTHQLASCT
ISDFAYWSSEQKNVQKYKIQTRINCCSWTIDGQYIALGLANGLVSIRNKFGEEKMKIDRG
KAPIYGLQWAPPSSTSGPVDILAIADWNQKLSFYTISGQIIGKERQLGFDPLCLSFFADG
ESLVVSGCNKQLQLYTRDGIRIGPLGEIHDSWIWSASVNPQGTAVVVGCQDGSLAYYNLT
FSTVHALYASRYAYRENMCDVIIQHLLSGQKVRIKCRDLVHKIAIYRTKLAVQLPERVVL
YELTAENQPMHYKVKEKISKKFNCSLLVVCSQNLVLCQEKRLQSLDFYGDLQREWIMDSF
IRYIKVTGGSPGSEGLIIGLKSGQVWRIFLDNSLPILLTTVLSSVRCLDLNATRTKLAVV
DDIGRMTVRDLTTDTLLYQDSGVNSLAWNTNHESMLCYSHTNGGLSIRVGSLPAKNPQNM
IGVVVGLCGATAFCLRGNLMTNIPLALSATLWQFVEAGLYEDAVEIANLGVPLNDWEALG
FAALDALNFKAAQDAFVKTRNLPWLEFINEILTRQKHIDISKELIQAEILAFSGKFKEAA
RLFQKCGKNNKAITMYCDLKMFDLAQQFLTNNEDGDDVNNSDSDDRRELVKKKAEWACSV
HEPRAAAELLLSAGEVERAIEIVAEQGWTDLLLDIGRKLQPTEKKSLELIANHLKELKAL
SMAAEIYRKLGEETQVVQLHIEARDWNEAFRLAEHLPQVLPTVHLSYAKWLVDSDQFIEA
HEAFVAANKPKEAHILLKNLADSAVTEERFLDASYYTWLRARQLMKMMEMNPEETSQHHI
KEFKVLLQLSAIYYAYATIFSYLREPFTSSPPLTLFNTSRFIANQIGNSTPPKGISMFNV
YYTLSKQAKVLGANKLHFQINNKLQQLKAPNGIQEQVDVNIMNSRGASTFSDPEDLLPMC
YKCSNFSMHLSGNNCPNCQQEFIFSFVSFEILPLAQFFPESDINEEEAERLLMTPPKENS
VDPFTETITQDDIGDLLPITLNRDALRSIDPRNVILVKTPKPIATKYYRNLLPELQITIC
PGCYQAFHSEDFELQVLQKGSCPFCRLPEDQLFDCY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g14435.t1 | Gene3D | G3DSA:2.130.10.10 | - | 3 | 123 | 0.0000e+00 |
| 12 | g14435.t1 | Gene3D | G3DSA:2.130.10.10 | - | 124 | 325 | 0.0000e+00 |
| 2 | g14435.t1 | PANTHER | PTHR12764 | WD REPEAT DOMAIN-RELATED | 1 | 1171 | 0.0000e+00 |
| 1 | g14435.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 51 | 88 | 1.0000e-07 |
| 14 | g14435.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 16 | 98 | 1.3316e+01 |
| 15 | g14435.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 57 | 88 | 1.4352e+01 |
| 11 | g14435.t1 | SMART | SM00320 | WD40_4 | 5 | 47 | 1.1000e+01 |
| 10 | g14435.t1 | SMART | SM00320 | WD40_4 | 50 | 89 | 0.0000e+00 |
| 8 | g14435.t1 | SMART | SM00320 | WD40_4 | 91 | 128 | 2.4000e+01 |
| 6 | g14435.t1 | SMART | SM00320 | WD40_4 | 130 | 168 | 1.3000e+01 |
| 7 | g14435.t1 | SMART | SM00320 | WD40_4 | 170 | 215 | 1.2000e+02 |
| 9 | g14435.t1 | SMART | SM00320 | WD40_4 | 217 | 256 | 3.8000e+02 |
| 5 | g14435.t1 | SMART | SM00320 | WD40_4 | 258 | 298 | 5.1000e-01 |
| 3 | g14435.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 21 | 299 | 0.0000e+00 |
| 4 | g14435.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 232 | 524 | 0.0000e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0060271 | cilium assembly | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K19656 (intraflagellar transport protein 122)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
