General information for g14460.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial.
Parent gene
- The parent gene g14460 is located on chr_4: 690083..690890 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14460 | g14460.t3 | TTS | g14460.t3 | 689477 | 689477 |
| chr_4 | g14460 | g14460.t3 | isoform | g14460.t3 | 689521 | 690549 |
| chr_4 | g14460 | g14460.t3 | exon | g14460.t3.exon1 | 689521 | 690549 |
| chr_4 | g14460 | g14460.t3 | cds | g14460.t3.CDS1 | 689592 | 690446 |
| chr_4 | g14460 | g14460.t3 | TSS | g14460.t3 | 690964 | 690964 |
Sequences
>g14460.t3 Gene=g14460 Length=1029
TCAGCTTTTTAAAGATTTAGAAAAGTCAGATAAATCACAGGTGATAATTCAAGGCTCTGG
AAAGGCATTCTGTGCAGGTCTTGACCTCAAGGAAGCTCTACAAATGAAATTTGATGAAAT
AAGCCGTCTTAATTTTTATACTTCAAACATCATGGATTACATTTCGCATTATAAAAAACC
TTACATTGCCCTTATTGATGGAATTTGTATGGGTGGTGCATGTTTCTTCTCTATGACTGC
CAAATATAGGATTGCAACTGAACGCACAATTTTCGCCATGCCAGAGGCTGAAATTGGATT
CTTTACTAATGCAGGTGCAAGTTATATCCTTCCAAGGCTCAAATATAATCTTGGCTTTTA
TATTGCACTTGCTGGTGCACGACTTTATGGTTATGAAACTAAGAAATTTGGACTTGCATC
GCATTTTGTTGAAAGCAAGCGAATGGATGAATTATTAAAAGCTTTAGCTGAATGCAATGA
TGATTATCAAGTTTCGAAAACAATTGCTAAATTTTCATCTATTCCGAATGATTTACATTG
TGATATAGATGAAATTTTATCATCAGTTGATAAATGCTTTGATGGTGACTCAGTTGAGGA
AATTGTTGATAATTTGCATCTTGATGGAAGTGAATGGGCAATGCAAACTGTTAGAACTCT
TAATAAAATGTCACCAACTAGTTTAAAAGTTTGTCATAAACTTTTGACAAATGGAAAGAA
ATTAAATTTGCAAGAGTGTTTAAAACTTGAACATTTATTGATGACAAATATTATGAGAGA
ATTTAATCATGATTTTATTGAAGGTGTTCGAGCTTTGATTCTTGAAAAAGATTTAAAACC
AAAATGGGAACCAAAAAAATTGCATGATGTTAAGGATGATTTATTGAGTAAAATATTTGC
TCCTATTCCAAAGGATAAGGAACTTACTTTTGAAAGCAATGAAAAGGCTAAACTGTAGAA
AAATTTGACAATAATTTTTAAATATTTCAACATAAAATATGGCCATCTTTACAAATAATA
TAAAAATGA
>g14460.t3 Gene=g14460 Length=284
MKFDEISRLNFYTSNIMDYISHYKKPYIALIDGICMGGACFFSMTAKYRIATERTIFAMP
EAEIGFFTNAGASYILPRLKYNLGFYIALAGARLYGYETKKFGLASHFVESKRMDELLKA
LAECNDDYQVSKTIAKFSSIPNDLHCDIDEILSSVDKCFDGDSVEEIVDNLHLDGSEWAM
QTVRTLNKMSPTSLKVCHKLLTNGKKLNLQECLKLEHLLMTNIMREFNHDFIEGVRALIL
EKDLKPKWEPKKLHDVKDDLLSKIFAPIPKDKELTFESNEKAKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g14460.t3 | CDD | cd06558 | crotonase-like | 15 | 123 | 4.75823E-27 |
| 4 | g14460.t3 | Gene3D | G3DSA:3.90.226.40 | - | 4 | 282 | 1.5E-80 |
| 2 | g14460.t3 | PANTHER | PTHR43176 | 3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED | 13 | 273 | 3.1E-82 |
| 1 | g14460.t3 | Pfam | PF16113 | Enoyl-CoA hydratase/isomerase | 16 | 265 | 6.5E-78 |
| 6 | g14460.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 26 | - |
| 7 | g14460.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 27 | 46 | - |
| 5 | g14460.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 47 | 284 | - |
| 3 | g14460.t3 | SUPERFAMILY | SSF52096 | ClpP/crotonase | 6 | 249 | 1.57E-35 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003860 | 3-hydroxyisobutyryl-CoA hydrolase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
