General information for g14466.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g14466 is located on chr_4: 711897..715034 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14466 | g14466.t1 | isoform | g14466.t1 | 711897 | 715034 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon1 | 711897 | 711919 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS1 | 711897 | 711919 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon2 | 711973 | 712229 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS2 | 711973 | 712229 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon3 | 712365 | 712398 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS3 | 712365 | 712398 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon4 | 713684 | 713846 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS4 | 713684 | 713846 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon5 | 714029 | 714174 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS5 | 714029 | 714174 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon6 | 714235 | 714346 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS6 | 714235 | 714346 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon7 | 714400 | 714534 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS7 | 714400 | 714534 |
| chr_4 | g14466 | g14466.t1 | exon | g14466.t1.exon8 | 714588 | 715034 |
| chr_4 | g14466 | g14466.t1 | cds | g14466.t1.CDS8 | 714588 | 715034 |
| chr_4 | g14466 | g14466.t1 | TSS | g14466.t1 | 715574 | 715574 |
| chr_4 | g14466 | g14466.t1 | TTS | g14466.t1 | NA | NA |
Sequences
>g14466.t1 Gene=g14466 Length=1317
ATGTCGAACCAAATTTCAATTCCTGAATTTTACAGTGGAAAAAATATATTTATAACAGGT
GGTACAGGATTTCTTGGCAAAGCAATTGTTGAGAAACTTTTACGATCATGTCCAGAAATT
GACCGAATTTATCTTCTTGCACGTGCAAAGAAGAACAAAACAGCTTCAGAAAGAATTCAG
GAAATCACAGATGCGCCACTTTTTGACGTTATTCGTGCTAAAAATCCGGAAATATTCAAG
AAAATTTCTTTATTAGAGGGTGATATTATCAAGGAAAATTTAGGTCTTTCAGATAATGAT
CTAAAAATATTTTATGAAAAAGTCAATGTCATAATTCATTCAGCAGCAACAATAAGTTTT
AATGAGCCATTAAAAATTGCAGTCAATACTAATTTACAACCAATAAAAGAGTTAATAAAA
TTGGCAAGAAAAACTAAAAGACTTGACTGTCTTATCCATATTTCAACAATTTATACAAAT
TGGTTTGAAAGAGATATAAGAGAAAAAATTTACAAGCCAAAATATGATCCAAATGAAATA
ATTGAAATGAGTAAAACCCTAACTGATGAAGAGCTAAACAAGATTTCATTAATGGGTCAT
GCAAATACTTATACATTTACAAAGTCATTAGCAGAATATCTTTTATCACAAGAAGGTCAT
GATTTACCAGCTGTAATTTTTAGACCATCAGTTGTTACAACAACTTTAAAAGAACCATTT
CCTGGATGGATTGATAATTGGGGTGGCACTGCACCAACACTTTTTATGACTTCGATAGGC
ATTTTTAAGCATCCTCGAATGGAAAAGGATTATATAATTGATTTAATACCTTGTGATACT
ACTGTCAATATGATTTTGGCTGTTGGTTGGAAAGTAGGAACTGAACAAAATGCTAAAAAT
TTACCAGTTGAAGCTTACAATTGCTCATCAAGCTCTCTTAATCCTATAAGTTATAAAGAA
TTATACAGTGGTTTTGTAGAAATGGGAAGAAAATATCCATACAATTTTACTTTAAAACTC
AAAGAGAAAAAGCCAAAACTAATAAAAATGATTGACACAACATATGACAATTACCATAAA
GTCAAATTTGCAACAACAACAAGAACAACTTTTCATAGTGAAAATCCAATAAAATTAATG
AAATTAATGAGCCAAAAAGATTTACAAGAATTTGATTTTGACGTGAGAAAAGTAAATTGG
AAAAGTTTCATTGAAACTTATTATTTAGGAATGCGACAATATTTAGGAAAAGAAAATCCG
ATAATTTTCCAATTCTACGAAAGAAAGTTCAAAGACTCAAGTTTAAAAATTATCTAG
>g14466.t1 Gene=g14466 Length=438
MSNQISIPEFYSGKNIFITGGTGFLGKAIVEKLLRSCPEIDRIYLLARAKKNKTASERIQ
EITDAPLFDVIRAKNPEIFKKISLLEGDIIKENLGLSDNDLKIFYEKVNVIIHSAATISF
NEPLKIAVNTNLQPIKELIKLARKTKRLDCLIHISTIYTNWFERDIREKIYKPKYDPNEI
IEMSKTLTDEELNKISLMGHANTYTFTKSLAEYLLSQEGHDLPAVIFRPSVVTTTLKEPF
PGWIDNWGGTAPTLFMTSIGIFKHPRMEKDYIIDLIPCDTTVNMILAVGWKVGTEQNAKN
LPVEAYNCSSSSLNPISYKELYSGFVEMGRKYPYNFTLKLKEKKPKLIKMIDTTYDNYHK
VKFATTTRTTFHSENPIKLMKLMSQKDLQEFDFDVRKVNWKSFIETYYLGMRQYLGKENP
IIFQFYERKFKDSSLKII
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g14466.t1 | CDD | cd05236 | FAR-N_SDR_e | 14 | 333 | 0 |
| 10 | g14466.t1 | CDD | cd09071 | FAR_C | 365 | 415 | 0 |
| 8 | g14466.t1 | Gene3D | G3DSA:3.40.50.720 | - | 3 | 409 | 0 |
| 3 | g14466.t1 | PANTHER | PTHR11011:SF60 | FATTY ACYL-COA REDUCTASE 2 | 6 | 333 | 0 |
| 5 | g14466.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 6 | 333 | 0 |
| 4 | g14466.t1 | PANTHER | PTHR11011:SF60 | FATTY ACYL-COA REDUCTASE 2 | 332 | 422 | 0 |
| 6 | g14466.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 332 | 422 | 0 |
| 1 | g14466.t1 | Pfam | PF07993 | Male sterility protein | 18 | 285 | 0 |
| 2 | g14466.t1 | Pfam | PF03015 | Male sterility protein | 334 | 418 | 0 |
| 7 | g14466.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 5 | 274 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed