General information for g14529.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable RNA-directed DNA polymerase from transposon X-element.
Parent gene
- The parent gene g14529 is located on chr_4: 1001048..1004915 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14529 | g14529.t2 | isoform | g14529.t2 | 1002399 | 1003884 |
| chr_4 | g14529 | g14529.t2 | exon | g14529.t2.exon1 | 1002399 | 1003797 |
| chr_4 | g14529 | g14529.t2 | cds | g14529.t2.CDS1 | 1002399 | 1003797 |
| chr_4 | g14529 | g14529.t2 | exon | g14529.t2.exon2 | 1003856 | 1003884 |
| chr_4 | g14529 | g14529.t2 | cds | g14529.t2.CDS2 | 1003856 | 1003884 |
| chr_4 | g14529 | g14529.t2 | TSS | g14529.t2 | NA | NA |
| chr_4 | g14529 | g14529.t2 | TTS | g14529.t2 | NA | NA |
Sequences
>g14529.t2 Gene=g14529 Length=1428
ATGAAACTAGCCCGCGATCCAGAATTCCTGATTTATCGACTGGACCGAGTTGATCAAGAA
AAAGGTGGTGTTGCAATTCTTGTCCGAAAGAATTTGAAACACAAAATCTTACCAATACCT
AACACCAAAATTATTGAATCCATCGGTATATCAGTTTTGCTCTCTAATGGCACAAATGCC
AACATTTTTAGCGTCTACCTCCCAGGTGGCACTAAGAACAGCATGATAAATGACCAATTT
ATCAATGATATCCGAATCTTGATGTCCTCGTCAAAGAATTCCTTTTTCTGCGGAGACCTA
AATGCTAAACACCGCTACTGGAACTGCAACATAGCAAATCGAGCAAGAACACTCCTATAC
AATGAGTTCTGTAATAAGAATTTTAATATCTACTTCCCACAAGGTAACACATATATACCA
AGCGACATAAATAGACAACCATCAACCATTGACCTGGTGCTCACAAACAGCAACTACGAC
ATCGAGCCCAAAATAAGCAACGAATTAGTTAGCGACCATACAGCTATTACTTTTGACATA
AACACTCATTCACCAGCTCAGTTAACAGAAAATAAGCGTCAACTAGATTATAGTAAAGCA
AATTGGCACAATTACAGACGCATGTTGATCAAATCACTTAACATTCAAAATAACCATGAA
ACAATAGCAACAGTCGATGACATTGACACTCAAATCGAAAAAATTACAACAGAAATTACA
AAAGCCAGAGATGCATCCATACCTAAGGTTATCTGCAACCAATATAAATTAAACCTACCA
GATTCACTGATCGGTTTAATTAAACGGAAAAATGCACTCAAGTGTGCCTGGAGAAGAAAT
AGAGTGCCTTCACTCAAAACAGAAATTAATTGTATGGAGAAAAAGATAAAGTATGAAATT
AGCCTAATCCGAAACAAAAACTGGGAGCACAAACTGAAAAGTATAAAGCCAAGTAATCAG
GCACTATGGAAAACCACTCGTATGTTGAAGAGTGGAAACAAAATTATTCCTCCATTGATG
ACAGCAGATGGACGGGTTATAAGAAGTGTTGATAAAGCTAATGTTATAGCAGACACTTTC
AAAGAAAATCACAAGAACCCACTATTTAAATCACCAACTGTTTATGATTACTCAATCAAA
ACAACAGTCGCAAACTATCTTAACAATAACACAAATGTTGATACACTAGACTCACTTTCG
CTCCCCACAATTGAGGAACTAAAAGAAGCAGTCAACAGGCTTCCCAACAACAAAGCACCG
GGAAAAGACGATATCAAAAACAGCCTGATTAAAAATCTTCCCAATATCGGATATGAGCTA
CTCCTCTCCATTTTCATTGCTTGCTTAAGACTGGGATACTTTCCTATGAGCTGGAAACAT
GTCAAAGTGTTTCCCATAGCACAATTCGTAAACTTCTTGAAAGATTAA
>g14529.t2 Gene=g14529 Length=475
MKLARDPEFLIYRLDRVDQEKGGVAILVRKNLKHKILPIPNTKIIESIGISVLLSNGTNA
NIFSVYLPGGTKNSMINDQFINDIRILMSSSKNSFFCGDLNAKHRYWNCNIANRARTLLY
NEFCNKNFNIYFPQGNTYIPSDINRQPSTIDLVLTNSNYDIEPKISNELVSDHTAITFDI
NTHSPAQLTENKRQLDYSKANWHNYRRMLIKSLNIQNNHETIATVDDIDTQIEKITTEIT
KARDASIPKVICNQYKLNLPDSLIGLIKRKNALKCAWRRNRVPSLKTEINCMEKKIKYEI
SLIRNKNWEHKLKSIKPSNQALWKTTRMLKSGNKIIPPLMTADGRVIRSVDKANVIADTF
KENHKNPLFKSPTVYDYSIKTTVANYLNNNTNVDTLDSLSLPTIEELKEAVNRLPNNKAP
GKDDIKNSLIKNLPNIGYELLLSIFIACLRLGYFPMSWKHVKVFPIAQFVNFLKD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 3 | g14529.t2 | Gene3D | G3DSA:3.60.10.10 | - | 2 | 183 | 3.7E-22 |
| 1 | g14529.t2 | Pfam | PF14529 | Endonuclease-reverse transcriptase | 61 | 176 | 2.4E-13 |
| 5 | g14529.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 435 | - |
| 6 | g14529.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 436 | 453 | - |
| 4 | g14529.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 454 | 475 | - |
| 2 | g14529.t2 | SUPERFAMILY | SSF56219 | DNase I-like | 8 | 182 | 5.5E-23 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed