General information for g14575.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as NAD-dependent protein deacylase Sirt4.
Parent gene
- The parent gene g14575 is located on chr_4: 1387140..1388157 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14575 | g14575.t1 | TTS | g14575.t1 | 1385841 | 1385841 |
| chr_4 | g14575 | g14575.t1 | isoform | g14575.t1 | 1385892 | 1387140 |
| chr_4 | g14575 | g14575.t1 | exon | g14575.t1.exon1 | 1385892 | 1386161 |
| chr_4 | g14575 | g14575.t1 | cds | g14575.t1.CDS1 | 1385892 | 1386161 |
| chr_4 | g14575 | g14575.t1 | exon | g14575.t1.exon2 | 1386240 | 1386730 |
| chr_4 | g14575 | g14575.t1 | cds | g14575.t1.CDS2 | 1386240 | 1386730 |
| chr_4 | g14575 | g14575.t1 | exon | g14575.t1.exon3 | 1386972 | 1387140 |
| chr_4 | g14575 | g14575.t1 | cds | g14575.t1.CDS3 | 1386972 | 1387140 |
| chr_4 | g14575 | g14575.t1 | TSS | g14575.t1 | 1387169 | 1387169 |
Sequences
>g14575.t1 Gene=g14575 Length=930
ATGAATTTAGTGAAAAAATTAAAAATTTCATCGAGAAATTATGTGACACAGTTTTTTATA
CCTAAACATGAGGCTGCCAACCCCAGAGATATTGAAATTATCCAAAATTACTTGTGTGAT
AAGCCCAAAATTTTAGTAGTCACTGGAGCTGGGATTTCAACTGAAAGTGGCATTCCTGAT
TACCGATCAGAAGGTGTCGGTCTTTATCAACGTTCAAATCACAAACCTGTTCAGTATCCA
GAATTTATTCGATCACATGAAATAAGAAAACGCTATTGGGCAAGAAATTTCCTTGGTTGG
CCAAAATTCAGCAATGTTCAACCAAATTTGACTCATAAAAAACTTGCTGAAATGGAAAAA
GACAAAAAATTGCTTCACATTGTAACACAAAATGTTGACAATTTACATGCAAAAGCAGGA
AGCAAAGAATTCACAGAGCTTCATGGGAATGGCTACAAAGTAATCTGCGTTGGTCATGAA
GGAAAAGATCGCTGCGGTTATTCGATAGATCGTCATGATTTTCAAAAAATTTTAGAATCT
TTCAATCAAGATTTGAAGGAAAAAGCTGAAATTATGAAATCTGAAAGTTTTCGACCTGAC
GGAGATGTTGAAATTTCATCGGATGATATTAAAAAATTTTATTTGCCGCAATGCCCTCAG
TGCATGGGAGATTTAAAGCCCAACATTGTCTTTTTTGGTGACAATATTCCGATGGATCGA
ATAGAAAAAGTTGTCAACTTGATCATTAATTCAGATGGAATTTTAGTACTTGGTTCATCA
CTTCAAGTGTTCAGTGGTTATCGAATAATTTTACAAGCAAAAGAACTTGGACTGCCTACT
GCAATTGTCAATATTGGTGCTACTAGAGCTGACCATTTAGTTGATATCAAAATTAATGCT
AAATGTAGTGATATTATGAAAGAATTGTAA
>g14575.t1 Gene=g14575 Length=309
MNLVKKLKISSRNYVTQFFIPKHEAANPRDIEIIQNYLCDKPKILVVTGAGISTESGIPD
YRSEGVGLYQRSNHKPVQYPEFIRSHEIRKRYWARNFLGWPKFSNVQPNLTHKKLAEMEK
DKKLLHIVTQNVDNLHAKAGSKEFTELHGNGYKVICVGHEGKDRCGYSIDRHDFQKILES
FNQDLKEKAEIMKSESFRPDGDVEISSDDIKKFYLPQCPQCMGDLKPNIVFFGDNIPMDR
IEKVVNLIINSDGILVLGSSLQVFSGYRIILQAKELGLPTAIVNIGATRADHLVDIKINA
KCSDIMKEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g14575.t1 | Gene3D | G3DSA:3.40.50.1220 | - | 41 | 304 | 0.000 |
| 5 | g14575.t1 | Gene3D | G3DSA:3.30.1600.10 | SIR2/SIRT2 ‘Small Domain’ | 58 | 234 | 0.000 |
| 2 | g14575.t1 | PANTHER | PTHR43688 | NAD-DEPENDENT PROTEIN LIPOAMIDASE SIRTUIN-4 | 15 | 308 | 0.000 |
| 1 | g14575.t1 | Pfam | PF02146 | Sir2 family | 49 | 264 | 0.000 |
| 6 | g14575.t1 | ProSiteProfiles | PS50305 | Sirtuin catalytic domain profile. | 32 | 309 | 40.623 |
| 3 | g14575.t1 | SUPERFAMILY | SSF52467 | DHS-like NAD/FAD-binding domain | 13 | 309 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0070403 | NAD+ binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11414 (NAD+-dependent protein deacetylase sirtuin 4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
