General information for g14587.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine protease snake.
Parent gene
- The parent gene g14587 is located on chr_4: 1420563..1422277 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14587 | g14587.t1 | isoform | g14587.t1 | 1417198 | 1422277 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon1 | 1417198 | 1417204 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon2 | 1419381 | 1420220 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS1 | 1419731 | 1420220 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon3 | 1420563 | 1420781 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS2 | 1420563 | 1420781 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon4 | 1420913 | 1421035 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS3 | 1420913 | 1421035 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon5 | 1421101 | 1421169 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS4 | 1421101 | 1421169 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon6 | 1421413 | 1421454 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS5 | 1421413 | 1421454 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon7 | 1421506 | 1421924 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS6 | 1421506 | 1421924 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon8 | 1422038 | 1422047 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS7 | 1422038 | 1422047 |
| chr_4 | g14587 | g14587.t1 | exon | g14587.t1.exon9 | 1422105 | 1422277 |
| chr_4 | g14587 | g14587.t1 | cds | g14587.t1.CDS8 | 1422105 | 1422277 |
| chr_4 | g14587 | g14587.t1 | TTS | g14587.t1 | 1422379 | 1422379 |
| chr_4 | g14587 | g14587.t1 | TSS | g14587.t1 | NA | NA |
Sequences
>g14587.t1 Gene=g14587 Length=1902
ATGATCTGAAATCAATACCATTGTCTAACAAATAATATACCAACAACATTGGGTAACATA
GTAACTAATGTTACAGGTACACATGAAAATGGCAAAACAAATGAAGATGTTACGGCAATA
TGGATTGAGGGAAATTACAAATTGTCATTTTTTCCACGTAATATTACATACTTTTTTCCA
AATATTAAAGGAATAAGATTCTATTCTTCATCAATTGACACACTCTATGGAAATGAGTTC
GATGAATTCGGTGAAAAACTTTTAGTGTTTGACTATTGGGTATCAAATTTGACAACAATT
TCAAGTCGATTGTTTGAAAAAATACCAAATTTGGTTACAATTTTATTAAATAATAATATG
AGAGAACGTGTTGGTCATGATTTATTTTCATCATTCAACATATCACAAATAAAAACATTT
GATTTTAGTAGAAATAGATGCATTGGTGGATGGAGATTGACAAACACAACAGAAATTAAA
GCAAAAATTGAGGAAATTAGAGAAAAATGTCCTTTCGATGATGAAAATTTAACAACAAAT
GCAATAATTTCTTCAACAACATCAAGTAACATTAAGACAACAACTAAAAATTCAAATTGC
ATTGAAAATAGTGTTGAAGAAATGTTCTGTACTTTAAAGAATGAAGTTTATGAAATTCAT
TATAATTTAGCAGCAAAAGATGACAAAATTAATGAAATAAAATCTGATCTTAATTACGCC
AAAGAAACATTTGAAAATCAGTTAATAAAAACCACAGAAGATTTTGAAAGTGTTAATAGA
GCTCATGAATCAAGAATTAAATGGTTAGAAGATGAAATTCTAAGATTATCAACAAATCCT
TGTGCATCTTCGGGTGAAGCATGTACAACATTTGATGGTTCAAGAGGTGTTTGCAAACAC
GTTCAAAGTTGTACAGAAATTGAAGAGTTAACAAAACTTCAGCCAAATGCCAAATTTACT
CGTTGTGATCGTTTCAAAAGACTTGTTTGTTGTCCAATCAAAAATTTTTCTGCAGAAAAT
GAAAATAAAATTTTTCAATCATCTTCTGTAAGAATCAGTGAAAGAAAATGTAGAGAATAT
GGAAAAAGTGTTATACAAGTGGTAAAATATTCCTCACTTATGTTGGGAGAACCTGATAAA
GAAACAATGATTAACAAATGCAAACATAAAGGAACTTCTTTAATTATTGGCGGAATAGAA
GCAGCAGAATCTGAATTCCCTCATCAAGCTCTTCTTGGTTATGATCGAGGACAATGGGTT
TGCGGAGGATCTTTGATTTCACCAAAATTTGTTTTAACAGCTGCTCATTGCATTCATTCA
AATTATTATGGTGATGTAAAATTAGTCAAAGTTGGATTAAACGATCGTTCGCAAATTGAA
GGAGTTTTAACTTTTGGTGTTGCAAAAACTTTTAAGTATCCAGAATTTAATGAAATATTG
CTCAATCATGATATTGCTTTGATAAAGTTAAACTCAACTGTTCGATTTAATGAATTTATT
TTACCTATTTGCTTACCAACTAGAGATTATGACGAAAACAAAGCAATTGTTTCTGGGTTT
GGCTCAATTGGAAATTATAATGGAGTTTCTGAAAAATTAATGAAAGTTACTCTAGAAAAG
TTTTCTTATGAAGACTGTCAAAATTCTTTTGAACGATTGATTGATAAAAATACAATGTTA
TGCTATGGACATCATACTGAGGAAAAAGATTCATGTTCCGGTGATTCCGGTGGTCCTTTG
CAAATTTACAATAAAGAAGTCAATTGTACTTACATCCAGTTAGGAATTGTAAGTTTTGCT
TCTACGGATAGTTGTGGATGGGTAGATTTACCAGCAGTCTACGTCAAAGTATACAATTAT
CTCGATTGGATTGAAAATATTGTTTGGAATGTAGAAGATTAA
>g14587.t1 Gene=g14587 Length=514
MRERVGHDLFSSFNISQIKTFDFSRNRCIGGWRLTNTTEIKAKIEEIREKCPFDDENLTT
NAIISSTTSSNIKTTTKNSNCIENSVEEMFCTLKNEVYEIHYNLAAKDDKINEIKSDLNY
AKETFENQLIKTTEDFESVNRAHESRIKWLEDEILRLSTNPCASSGEACTTFDGSRGVCK
HVQSCTEIEELTKLQPNAKFTRCDRFKRLVCCPIKNFSAENENKIFQSSSVRISERKCRE
YGKSVIQVVKYSSLMLGEPDKETMINKCKHKGTSLIIGGIEAAESEFPHQALLGYDRGQW
VCGGSLISPKFVLTAAHCIHSNYYGDVKLVKVGLNDRSQIEGVLTFGVAKTFKYPEFNEI
LLNHDIALIKLNSTVRFNEFILPICLPTRDYDENKAIVSGFGSIGNYNGVSEKLMKVTLE
KFSYEDCQNSFERLIDKNTMLCYGHHTEEKDSCSGDSGGPLQIYNKEVNCTYIQLGIVSF
ASTDSCGWVDLPAVYVKVYNYLDWIENIVWNVED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g14587.t1 | CDD | cd00190 | Tryp_SPc | 276 | 508 | 5.66317E-75 |
| 8 | g14587.t1 | Gene3D | G3DSA:2.40.10.10 | - | 267 | 509 | 2.2E-65 |
| 2 | g14587.t1 | PANTHER | PTHR24260:SF107 | EG:BACR7A4.3 PROTEIN-RELATED | 160 | 513 | 9.5E-66 |
| 3 | g14587.t1 | PANTHER | PTHR24260 | - | 160 | 513 | 9.5E-66 |
| 4 | g14587.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 303 | 318 | 4.5E-15 |
| 5 | g14587.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 361 | 375 | 4.5E-15 |
| 6 | g14587.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 450 | 462 | 4.5E-15 |
| 1 | g14587.t1 | Pfam | PF00089 | Trypsin | 276 | 505 | 1.0E-49 |
| 10 | g14587.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 313 | 318 | - |
| 11 | g14587.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 451 | 462 | - |
| 15 | g14587.t1 | ProSiteProfiles | PS51888 | Clip domain profile. | 168 | 212 | 9.964 |
| 14 | g14587.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 276 | 510 | 28.232 |
| 13 | g14587.t1 | SMART | SM00680 | clip | 169 | 213 | 0.0018 |
| 12 | g14587.t1 | SMART | SM00020 | trypsin_2 | 275 | 505 | 5.5E-69 |
| 7 | g14587.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 269 | 511 | 2.62E-71 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed