General information for g14653.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Heterochromatin-associated protein MENT.
Parent gene
- The parent gene g14653 is located on chr_4: 1756337..1758189 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14653 | g14653.t1 | isoform | g14653.t1 | 1756337 | 1758189 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon1 | 1756337 | 1756346 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS1 | 1756337 | 1756346 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon2 | 1756402 | 1756514 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS2 | 1756402 | 1756514 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon3 | 1756901 | 1757117 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS3 | 1756901 | 1757117 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon4 | 1757170 | 1757777 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS4 | 1757170 | 1757777 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon5 | 1757857 | 1758007 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS5 | 1757857 | 1758007 |
| chr_4 | g14653 | g14653.t1 | exon | g14653.t1.exon6 | 1758065 | 1758189 |
| chr_4 | g14653 | g14653.t1 | cds | g14653.t1.CDS6 | 1758065 | 1758189 |
| chr_4 | g14653 | g14653.t1 | TSS | g14653.t1 | NA | NA |
| chr_4 | g14653 | g14653.t1 | TTS | g14653.t1 | NA | NA |
Sequences
>g14653.t1 Gene=g14653 Length=1224
ATGACACGTAATTTTCTGAGCATATTTTTACTTATTTTGAGTGTCAATTTTGTTGCCTCT
CAAGGCAATCTTGACAGTGGATTTGTTAAAGCCTTGACATCATTTGCAATTCAATTATTT
CAGGAAGTGACGGCAACAATTTCTGATAATGCGATGATTTCACCACTTTCTGTTCAATCA
ATTTTATCATTAGCAATGTATGGAGCAAAAGGCGAGACGAAATTAACAATGTTTGACACC
ATGACTTACAGCAATTTTAAAAATGTTTCATATGAAACGGTTGAGAAAAATTTCAAAGAA
CTTGTGAATAATTTCACAGGAAGTCGAGCACTTGTCATGGCATTTAAAATTTATGCAACA
AATCTTTTTCAAATTTCTCCTGAATTTCAACAAATTGCAATTGATTCTTTTTCATCTGAA
ATTGAGTCGATGAGTTTTAGTTATGAAGACACAACACCTGAGAAAGTTGCAGCATATATC
AATGCATGGATTGCAAATAAAACAAGAAATCGAATTTTAGATGCTGTCAGTCCTTATCAA
TTTGAAAAACTGAATTCAACTAGATTGGTGCTTGTTAATACAATTTATTTTAAAGACAAT
TGGTTAATAATGTTTGATTCTGGAGCAACACATCGTGATAAATTTTATATCAATGCTAAG
AAATATATTATGACAAAATTTATGGTAAAATCTGAAGATTATCTGTATGCAAAGTTTCCT
GAATTAAATGCATCAGCTTTAATTTTAACCTATGAAAAGTCACCAATGGAAATGATAATT
TTGCTACCAAATGCAAGGACTGGACTGAATACTCTAAAACAAAATTTGCAAACAATTGAT
TGGAACAGCAAAAGCGTCAAAAAACGTTTTAAAATTACACCGACGGATTTAAAAATTCCA
AAATGGCAATTTGAATTTAATATTGAACTAAAAGATTATTTAAAAAATATGGGCATGTTA
CTTCCATTTAGTGAATTAGCTGATTTTAATGGAATATTTGAAAGTTTGGATAATAAAACA
AATAGTAAAATATCAAAAGTTATTCATAAAACTTTTATTAATGTCAATGAAAGAGGAACT
GAAGCTTCTGCAGCTTCTGTTATGACTATGGAAGCAACAAGCCTTACTTTAAATCCACAA
ATATTTCATGTCGATCATCCTTTTATTTTTATGCTTCGTTATTATAACAGCATTTGCTTT
ATTGGACAAGTTACGCAATTTTGA
>g14653.t1 Gene=g14653 Length=407
MTRNFLSIFLLILSVNFVASQGNLDSGFVKALTSFAIQLFQEVTATISDNAMISPLSVQS
ILSLAMYGAKGETKLTMFDTMTYSNFKNVSYETVEKNFKELVNNFTGSRALVMAFKIYAT
NLFQISPEFQQIAIDSFSSEIESMSFSYEDTTPEKVAAYINAWIANKTRNRILDAVSPYQ
FEKLNSTRLVLVNTIYFKDNWLIMFDSGATHRDKFYINAKKYIMTKFMVKSEDYLYAKFP
ELNASALILTYEKSPMEMIILLPNARTGLNTLKQNLQTIDWNSKSVKKRFKITPTDLKIP
KWQFEFNIELKDYLKNMGMLLPFSELADFNGIFESLDNKTNSKISKVIHKTFINVNERGT
EASAASVMTMEATSLTLNPQIFHVDHPFIFMLRYYNSICFIGQVTQF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g14653.t1 | CDD | cd19601 | serpin42Da-like | 31 | 403 | 8.38525E-107 |
| 7 | g14653.t1 | Gene3D | G3DSA:3.30.497.10 | Antithrombin | 48 | 352 | 2.1E-86 |
| 6 | g14653.t1 | Gene3D | G3DSA:2.30.39.10 | - | 197 | 402 | 2.1E-86 |
| 2 | g14653.t1 | PANTHER | PTHR11461 | SERINE PROTEASE INHIBITOR, SERPIN | 30 | 405 | 2.6E-64 |
| 3 | g14653.t1 | PANTHER | PTHR11461:SF180 | LEUKOCYTE ELASTASE INHIBITOR | 30 | 405 | 2.6E-64 |
| 1 | g14653.t1 | Pfam | PF00079 | Serpin (serine protease inhibitor) | 31 | 405 | 1.6E-75 |
| 9 | g14653.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 10 | g14653.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 11 | g14653.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 15 | - |
| 12 | g14653.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 8 | g14653.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 407 | - |
| 15 | g14653.t1 | ProSitePatterns | PS00284 | Serpins signature. | 382 | 392 | - |
| 16 | g14653.t1 | SMART | SM00093 | serpin2 | 37 | 407 | 3.7E-51 |
| 4 | g14653.t1 | SUPERFAMILY | SSF56574 | Serpins | 16 | 405 | 6.94E-86 |
| 5 | g14653.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 14 | g14653.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005615 | extracellular space | CC |
| GO:0004867 | serine-type endopeptidase inhibitor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed