General information for g14654.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serpin-Z1A.
Parent gene
- The parent gene g14654 is located on chr_4: 1758512..1761034 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14654 | g14654.t1 | isoform | g14654.t1 | 1758512 | 1761034 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon1 | 1758512 | 1758524 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS1 | 1758512 | 1758524 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon2 | 1758585 | 1758697 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS2 | 1758585 | 1758697 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon3 | 1759255 | 1759471 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS3 | 1759255 | 1759471 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon4 | 1759750 | 1760357 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS4 | 1759750 | 1760357 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon5 | 1760511 | 1760661 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS5 | 1760511 | 1760661 |
| chr_4 | g14654 | g14654.t1 | exon | g14654.t1.exon6 | 1760910 | 1761034 |
| chr_4 | g14654 | g14654.t1 | cds | g14654.t1.CDS6 | 1760910 | 1761034 |
| chr_4 | g14654 | g14654.t1 | TSS | g14654.t1 | NA | NA |
| chr_4 | g14654 | g14654.t1 | TTS | g14654.t1 | NA | NA |
Sequences
>g14654.t1 Gene=g14654 Length=1227
ATGATAAAGAGCAAAATATTAATCATTTTGCTTATTGTTTCATGTGCAAGTTTTGTGACA
TCTCAAGGTTACCTTGATAATGGATTTGTAAAAGCTTTATCAAGTTTTGCTATCCGACTG
TATCAGGAAGTAACCACATCAATTTCTGACAATGTTATGCTTTCACCCCTATCAATACAA
TCAATTTTATCATTAACAATGTACGGTGCAAAAGGTGATACGAAACAAGCAATGTTTGAC
ACAATGACCTACAGTTCGTTTGAAAATATGACTTATGAAACAGTTGCAATGAATTTTAAA
GAACTTATTAATGGACTTGAAGGAAGTAAGGCAATTTTTACAGCATTCAAAATTTACGTC
ACAGATCTCTACAATTTGTCATCTGACTATCAAAAAATTGCAATTGATTATTTCTCATCT
GAAATTGAATCGATGAGTTTTCGTTTTGAAGACACAACACCTGAGAAAGTTGCAGCTTAC
ATAAATTCTTGGGTAGCAAGGAAAACTAAAAATAGAATTCAAGACCTTATAACACCATAT
TCACTAAAATATTTAAATACTACAAGATTAATTTTGATCAATACAATTTATTTCAAAGAC
ACTTGGACAAAACTTTTTACACAAGCAAATACAAAGCCAGGAAAATTTTATATAAGCGCT
ACTAAATCTGTTACAGCTAATTTTATGTCATTGAAAAGTAATTTTCTTTATGCAACATTA
CCTGAATTAAATGCAACAGCTATCACTTTAAGCTATAAAAATTCACCAATGGAAATGATA
ATTTTGCTACCAAATGCAAGAAATGGATTGAAAATAATGAAAAATTTATTAGACATAGTT
GATTGGACTAGTGAAAATGTAACAAACCGATTTCAGTATAGCATAGTGAATCTAAAAATT
CCAAAATGGAAATTTGAATTTTCTGTTGATCTTGGAAATTTTTTGAAAGACATGGGCATG
TCGTTACCATTTACAGAAGATGCAGATTTTACAGGAATGCTTGACAACTATAATGAAAAT
TGTACTATAAGAATCAGCAAAGTTGTACACAAAACTTTTATTTCTGTCAACGAAAATGGA
ACTGAAGCTTCTGCAGCTACAGCGGTTTATATGATGGGATCTGGTGGACCATCAAAACCA
AAAGATTTCATTGCTGATCATCCTTTCATTTTTATGCTTCGATACAATTTAAATATTTAT
TTTATTGGACAACTTACACAATTTTAA
>g14654.t1 Gene=g14654 Length=408
MIKSKILIILLIVSCASFVTSQGYLDNGFVKALSSFAIRLYQEVTTSISDNVMLSPLSIQ
SILSLTMYGAKGDTKQAMFDTMTYSSFENMTYETVAMNFKELINGLEGSKAIFTAFKIYV
TDLYNLSSDYQKIAIDYFSSEIESMSFRFEDTTPEKVAAYINSWVARKTKNRIQDLITPY
SLKYLNTTRLILINTIYFKDTWTKLFTQANTKPGKFYISATKSVTANFMSLKSNFLYATL
PELNATAITLSYKNSPMEMIILLPNARNGLKIMKNLLDIVDWTSENVTNRFQYSIVNLKI
PKWKFEFSVDLGNFLKDMGMSLPFTEDADFTGMLDNYNENCTIRISKVVHKTFISVNENG
TEASAATAVYMMGSGGPSKPKDFIADHPFIFMLRYNLNIYFIGQLTQF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g14654.t1 | CDD | cd19601 | serpin42Da-like | 32 | 404 | 1.30359E-115 |
| 8 | g14654.t1 | Gene3D | G3DSA:3.30.497.10 | Antithrombin | 38 | 373 | 3.7E-95 |
| 7 | g14654.t1 | Gene3D | G3DSA:2.30.39.10 | - | 203 | 404 | 3.7E-95 |
| 2 | g14654.t1 | PANTHER | PTHR11461 | SERINE PROTEASE INHIBITOR, SERPIN | 31 | 406 | 2.8E-69 |
| 3 | g14654.t1 | PANTHER | PTHR11461:SF180 | LEUKOCYTE ELASTASE INHIBITOR | 31 | 406 | 2.8E-69 |
| 1 | g14654.t1 | Pfam | PF00079 | Serpin (serine protease inhibitor) | 32 | 406 | 2.9E-82 |
| 10 | g14654.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 11 | g14654.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 12 | g14654.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 16 | - |
| 13 | g14654.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 9 | g14654.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 408 | - |
| 16 | g14654.t1 | ProSitePatterns | PS00284 | Serpins signature. | 383 | 393 | - |
| 17 | g14654.t1 | SMART | SM00093 | serpin2 | 38 | 408 | 5.5E-60 |
| 4 | g14654.t1 | SUPERFAMILY | SSF56574 | Serpins | 9 | 405 | 4.06E-92 |
| 6 | g14654.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 5 | g14654.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 25 | - |
| 15 | g14654.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 25 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005615 | extracellular space | CC |
| GO:0004867 | serine-type endopeptidase inhibitor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed