General information for g14656.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Peroxidase.
Parent gene
- The parent gene g14656 is located on chr_4: 1767540..1770357 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14656 | g14656.t1 | isoform | g14656.t1 | 1767540 | 1770357 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon1 | 1767540 | 1767600 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS1 | 1767540 | 1767600 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon2 | 1767656 | 1768288 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS2 | 1767656 | 1768288 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon3 | 1768715 | 1769507 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS3 | 1768715 | 1769507 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon4 | 1769592 | 1769849 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS4 | 1769592 | 1769849 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon5 | 1770154 | 1770225 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS5 | 1770154 | 1770225 |
| chr_4 | g14656 | g14656.t1 | exon | g14656.t1.exon6 | 1770288 | 1770357 |
| chr_4 | g14656 | g14656.t1 | cds | g14656.t1.CDS6 | 1770288 | 1770357 |
| chr_4 | g14656 | g14656.t1 | TSS | g14656.t1 | NA | NA |
| chr_4 | g14656 | g14656.t1 | TTS | g14656.t1 | NA | NA |
Sequences
>g14656.t1 Gene=g14656 Length=1887
ATGAAAGAAAAATCAAAAAATGTTGGAAAAATTAGTAATTTTCAAATAACTGTAGCTTTG
AGTGTTCTTGCTCTTTCAGTTTTTGTGTTTGCTGGTGGTCCATCGTTTTTTAATTCAATT
AATATTATTCCAAGTTTGAGAGACGCAATATGCCTACAACAATCAACTCAACCAAAATGC
ACTCCATCAAAATTTAGATCACTCGATGGCTCTTGCAATAATCCAGATCATCCTAATTGG
GGTATGGCAAATTTACCATATGGAAGACTTTTACCTGCTTGGTATAGTGACAATTATTCT
GCACATCCAAAATCTTTTACTGGAAATGATTTGCCAAATCCTAGAATAATTTCAAAAATT
GTTTTTGGTGAAGAAAGTTTTCCTGATCCTGAATATACAATTAGTATGATGCAATTTGGT
CAATTTATGGCACATGATATGGGAAATACACTTCGGAGCTCTAAACAATCATGCTGTTCT
TATTCAGGACGCTTTTTGTCAAACTCTCCATCATGTTCTGACATTCATATTCCAGAAACT
GATGCTTTTTATGGTCCTCGTAATATAAATTGTATGAATTTTTCTGTCACAGTTTCTGAT
CATGATTTAGAATGCAAAGGTGTTGAATCAACTCCAGCAAATCCAATTTCATCTGTGACA
CCTTTTTTCGATTTGTCATTAATTTATGGAAATAGTGACGAGGAAGCAAGATTAATTCGA
TCATTTAAAAGTGGTTGCTTGACAATTGAAATCATTGATGGTTATGAATGGCCTCCACAT
GATCCTAATGCAACTACAAGTTGTTCTATTGATTCTGAAACTGATCCATGCTTTTTGGGC
GGTGATACAAGAATAAATCAAAGTCCACATTTGACAATTATTCATGTTTTATTTATTCGT
GAGCACAATCGAATTGCTCGTGAGTTGGAAAAATTAAATCCACATTGGAGTGATGAAAAA
TTATATCAAGAAGCAAGAAGAATTTGCATTGGAATTTATCAATATATTTCATATTATGAA
TGGCTAACAATTTTTTTAGGAACACAAAACATGTTAAATCTTGGAATGATTACACATTTT
GATGGCGATGAATATGCTAATGATTTTGATTCTAGCGTTAATCCAGCTGTTTTCAATGAA
CATTCACAAGCTGCTTTTAGATTTTATCATACACAGATTGAGGGTCATTTACAGCTTCAA
ACAGAATCTCGTGACATTATCGACACAATAGCCATAAGCGATCATTTTGACAATCCAAAT
TTTACTTTATATCATTTTGATGAACTTACTCGAGGAATGATAACACAAATGTCTCAATTA
ACAGACAGTAATTTTGATGAGCAAATTCGTGAATTTTTATTCAAGCATAAGCGAGATTAT
GGTGATGATTTGAGATCAATTGACATTACAAGAGGCAGAGATCATGGACTTGCAAGTTAT
AATAGTTTTAGAGATTTTTGTGGACTTCCAAAAGCAACAAAATGGGAAGATTATTTGGAT
TTAATTTCTCTAATTGATTTGCAACATTTACAAGAAGTTTATGAAGATTACAGAGATGTT
GAACTTTCAGTTGGTGCTTTGCTTGAAGCACAAATTGATAACACAACACTTGCTGGTCCA
ACTTTATTATGCATTTTTAATATTCAGTTTCTAAATTCAAAAATTGGTGATCGTTATTGG
TTTGAAAGTGGCGATCCTGAAATTGCTTTTACTCGTCCACAATTGGCTGAAATTAGAAAA
TCAAGTTTTGCTAGAATTGTTTGTGACAATAGTCATAATGTTACAAGTTCTCAACTTTTG
GCTTTTCGAACTGTCAGAAAAGATACGAATCCAATTGTTCCATGCACAGAAATACCATTT
GTTAATTTAACATTTTGGACTGAATAA
>g14656.t1 Gene=g14656 Length=628
MKEKSKNVGKISNFQITVALSVLALSVFVFAGGPSFFNSINIIPSLRDAICLQQSTQPKC
TPSKFRSLDGSCNNPDHPNWGMANLPYGRLLPAWYSDNYSAHPKSFTGNDLPNPRIISKI
VFGEESFPDPEYTISMMQFGQFMAHDMGNTLRSSKQSCCSYSGRFLSNSPSCSDIHIPET
DAFYGPRNINCMNFSVTVSDHDLECKGVESTPANPISSVTPFFDLSLIYGNSDEEARLIR
SFKSGCLTIEIIDGYEWPPHDPNATTSCSIDSETDPCFLGGDTRINQSPHLTIIHVLFIR
EHNRIARELEKLNPHWSDEKLYQEARRICIGIYQYISYYEWLTIFLGTQNMLNLGMITHF
DGDEYANDFDSSVNPAVFNEHSQAAFRFYHTQIEGHLQLQTESRDIIDTIAISDHFDNPN
FTLYHFDELTRGMITQMSQLTDSNFDEQIREFLFKHKRDYGDDLRSIDITRGRDHGLASY
NSFRDFCGLPKATKWEDYLDLISLIDLQHLQEVYEDYRDVELSVGALLEAQIDNTTLAGP
TLLCIFNIQFLNSKIGDRYWFESGDPEIAFTRPQLAEIRKSSFARIVCDNSHNVTSSQLL
AFRTVRKDTNPIVPCTEIPFVNLTFWTE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g14656.t1 | CDD | cd09823 | peroxinectin_like | 213 | 590 | 4.6762E-165 |
| 12 | g14656.t1 | Gene3D | G3DSA:1.10.640.10 | Myeloperoxidase | 47 | 628 | 3.5E-172 |
| 2 | g14656.t1 | PANTHER | PTHR11475 | OXIDASE/PEROXIDASE | 49 | 626 | 1.6E-232 |
| 3 | g14656.t1 | PANTHER | PTHR11475:SF86 | PEROXIDASE | 49 | 626 | 1.6E-232 |
| 9 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 89 | 100 | 1.1E-29 |
| 10 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 135 | 150 | 1.1E-29 |
| 8 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 278 | 296 | 1.1E-29 |
| 7 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 296 | 316 | 1.1E-29 |
| 6 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 321 | 347 | 1.1E-29 |
| 5 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 383 | 393 | 1.1E-29 |
| 4 | g14656.t1 | PRINTS | PR00457 | Animal haem peroxidase signature | 578 | 592 | 1.1E-29 |
| 1 | g14656.t1 | Pfam | PF03098 | Animal haem peroxidase | 65 | 600 | 3.4E-163 |
| 14 | g14656.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 31 | - |
| 15 | g14656.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 15 | - |
| 16 | g14656.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 16 | 27 | - |
| 17 | g14656.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 28 | 31 | - |
| 13 | g14656.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 32 | 628 | - |
| 20 | g14656.t1 | ProSiteProfiles | PS50292 | Animal heme peroxidase superfamily profile. | 57 | 628 | 127.204 |
| 11 | g14656.t1 | SUPERFAMILY | SSF48113 | Heme-dependent peroxidases | 60 | 628 | 1.77E-156 |
| 19 | g14656.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 34 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006979 | response to oxidative stress | BP |
| GO:0020037 | heme binding | MF |
| GO:0004601 | peroxidase activity | MF |
| GO:0042600 | egg chorion | CC |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
