General information for g14672.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase .
Parent gene
- The parent gene g14672 is located on chr_4: 1845584..1846484 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14672 | g14672.t1 | TSS | g14672.t1 | 1845218 | 1845218 |
| chr_4 | g14672 | g14672.t1 | isoform | g14672.t1 | 1845256 | 1846484 |
| chr_4 | g14672 | g14672.t1 | exon | g14672.t1.exon1 | 1845256 | 1845417 |
| chr_4 | g14672 | g14672.t1 | cds | g14672.t1.CDS1 | 1845256 | 1845417 |
| chr_4 | g14672 | g14672.t1 | exon | g14672.t1.exon2 | 1845477 | 1845537 |
| chr_4 | g14672 | g14672.t1 | cds | g14672.t1.CDS2 | 1845477 | 1845537 |
| chr_4 | g14672 | g14672.t1 | exon | g14672.t1.exon3 | 1845592 | 1846097 |
| chr_4 | g14672 | g14672.t1 | cds | g14672.t1.CDS3 | 1845592 | 1846097 |
| chr_4 | g14672 | g14672.t1 | exon | g14672.t1.exon4 | 1846167 | 1846484 |
| chr_4 | g14672 | g14672.t1 | cds | g14672.t1.CDS4 | 1846167 | 1846484 |
| chr_4 | g14672 | g14672.t1 | TTS | g14672.t1 | 1846553 | 1846553 |
Sequences
>g14672.t1 Gene=g14672 Length=1047
ATGAAAAGATTCATTTTACTTTTATCTCCATTAATTAATCTCATTTCACCATCAATAATC
TCATCAGATGAGCAAAGAATTCCAATAGTTATAAATACATGGGACTTTACAAATGCTACA
GTAAAAGCATGGGATGTGATTAATAGACAGAAAAAATCAGCGATTGATGCTATTGTTAAA
GGTTGTAGTGTATGTGAAAGGGAACAATGTGATGGAACTGTTGGCTACGGCGGATCACCA
AATGAAAATGGTGAAACATGTTTGGATGCATTCTTAATGGATGGTCGCACAATGAATATT
GGTGCAGTTGGTAGTATAAGGAATATAAAGGATGCAATAGCTGTAGCAAAACATGTTTTA
CTTTATACCAAACATTCTTTATTAGTTGGAGAGCAAGCGACTCAATTTGCTCTTATGATG
GGCTTTAAAAAACAAAGTTTAAGCACTGAAAAATCAAAAGCAATGCATCGTGATTGGAAA
AAGAAAAACTGCCAGCCAAATTTTTGGACAAATGTCCTGCCATCTCCGGAAAACAGTTGT
GGTCCATATGTGCCAATGAGTGAAGATTTTTTGACTTCAGCTCATGAATATCAAAGTTTT
TTCTCAAATTATAATCATGACACAATCGGAATGGTTGCAATTGATGTTAATGGAAATATT
GCAACTGGAACATCGACAAATGGAGTGAACCATAAAATTCCAGGTCGAGTCGGAGATTCT
CCTATTCCTGGTGCTGGTGGTTATGCTGATAATGCAGTTGGTGCTGCTGCAGCGACAGGT
GATGGTGATATTTTGATGAGATTTTTGCCTTCAATGTTGGCTGTTGAATTTATGAGAAAT
GGAATGACACCAGATGAAGCTGGAAGAAAAGCATTGGCAAGAATTTCTCATCATTATCCA
AAATTTGTAGGTGCAATTGTTGTAGTTGATAAAAATGGCAATTATGGAGCTGCATGTCAT
GGTATTGACTCATTTCCTTTTAGTGTTTATCATTCAAAACTAAAAAATGTTAAGGTTGAA
AAACGCGAATGTGCTATGAGCATTTAA
>g14672.t1 Gene=g14672 Length=348
MKRFILLLSPLINLISPSIISSDEQRIPIVINTWDFTNATVKAWDVINRQKKSAIDAIVK
GCSVCEREQCDGTVGYGGSPNENGETCLDAFLMDGRTMNIGAVGSIRNIKDAIAVAKHVL
LYTKHSLLVGEQATQFALMMGFKKQSLSTEKSKAMHRDWKKKNCQPNFWTNVLPSPENSC
GPYVPMSEDFLTSAHEYQSFFSNYNHDTIGMVAIDVNGNIATGTSTNGVNHKIPGRVGDS
PIPGAGGYADNAVGAAAATGDGDILMRFLPSMLAVEFMRNGMTPDEAGRKALARISHHYP
KFVGAIVVVDKNGNYGAACHGIDSFPFSVYHSKLKNVKVEKRECAMSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g14672.t1 | CDD | cd04513 | Glycosylasparaginase | 29 | 330 | 2.95458E-170 |
| 6 | g14672.t1 | Gene3D | G3DSA:3.60.20.30 | (Glycosyl)asparaginase | 208 | 345 | 1.4E-51 |
| 2 | g14672.t1 | PANTHER | PTHR10188:SF6 | N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE | 17 | 339 | 6.2E-144 |
| 3 | g14672.t1 | PANTHER | PTHR10188 | L-ASPARAGINASE | 17 | 339 | 6.2E-144 |
| 1 | g14672.t1 | Pfam | PF01112 | Asparaginase | 35 | 337 | 1.5E-84 |
| 8 | g14672.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 9 | g14672.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 10 | g14672.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 11 | g14672.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 22 | - |
| 7 | g14672.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 348 | - |
| 4 | g14672.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 28 | 334 | 8.49E-95 |
| 5 | g14672.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01444 (N4-(beta-N-acetylglucosaminyl)-L-asparaginase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
