General information for g14776.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as ADP-ribosylation factor 2.
Parent gene
- The parent gene g14776 is located on chr_4: 2274660..2282596 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14776 | g14776.t2 | TSS | g14776.t2 | 2272211 | 2272211 |
| chr_4 | g14776 | g14776.t2 | isoform | g14776.t2 | 2272292 | 2275005 |
| chr_4 | g14776 | g14776.t2 | exon | g14776.t2.exon1 | 2272292 | 2272374 |
| chr_4 | g14776 | g14776.t2 | exon | g14776.t2.exon2 | 2272648 | 2272725 |
| chr_4 | g14776 | g14776.t2 | cds | g14776.t2.CDS1 | 2272659 | 2272725 |
| chr_4 | g14776 | g14776.t2 | exon | g14776.t2.exon3 | 2273099 | 2273487 |
| chr_4 | g14776 | g14776.t2 | cds | g14776.t2.CDS2 | 2273099 | 2273487 |
| chr_4 | g14776 | g14776.t2 | exon | g14776.t2.exon4 | 2274574 | 2274776 |
| chr_4 | g14776 | g14776.t2 | cds | g14776.t2.CDS3 | 2274574 | 2274660 |
| chr_4 | g14776 | g14776.t2 | exon | g14776.t2.exon5 | 2274882 | 2275005 |
| chr_4 | g14776 | g14776.t2 | TTS | g14776.t2 | 2275004 | 2275004 |
Sequences
>g14776.t2 Gene=g14776 Length=877
ACTGAAAAGTTAGAAAAATCAACAAACAAAACAACCAAAAGTCAATCACAGAATTTAGTT
ACAAGGAATTTTTCAAAATTAAGTGCATTCAAAAATGGGTCTCACAATTTCAAGTGTCTT
CAATCGACTTTTTGGAAAAAAGCAGATGAGGATTTTAATGGTCGGTCTCGATGCAGCTGG
TAAAACCACAATTCTCTACAAATTAAAACTCGGTGAAATTGTCACAACCATTCCAACAAT
TGGTTTCAATGTCGAGACAGTAGAATACAAAAACATTTGTTTCACAGTGTGGGATGTTGG
TGGTCAAGATAAAATTCGTCCACTTTGGCGTCATTATTTCCAAAACACTCAAGGTCTCAT
TTTTGTCGTTGATTCAAATGATCGTGAGCGTGTTGTTGAAGCTGAAAGGGAACTTCATAA
TATGTTGAATGAAGATGAACTTCGTGATGCAGTGATTTTGGTATTTGCAAACAAACAAGA
TTTGCCAAATGCAATGAGCGCATCGGAATTGACTGAAAAATTAAGGTTGAATTCACTGCA
TAATAGAAGTTGGTATATTCAGGCAACATGTGCAACACAAGGAAATGGTCTCTATGAAGG
ACTCGATTGGCTGTCAAAGGAACTTGAAAAGAAATAAACAAAACAATAATCAAAAACACT
AAAAATTACTTTAAATTAAATTGAAATGAAGACAAGACCAGTCCATCACTACCTTTCCTA
AATTTCTCTTTTTCCTATTCTGCTCAATTAAAATAAAATATTTTTTTGTTTAAATTATGT
TATCAAAATCAACATTTTCATGCATGGCAATTTTTCTTCTTATTAATTAAATATGATTAT
CTGTAATAAATTAAAAATTGAATAAAATGTGTAAAAA
>g14776.t2 Gene=g14776 Length=180
MGLTISSVFNRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN
ICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVVEAERELHNMLNEDELRDAV
ILVFANKQDLPNAMSASELTEKLRLNSLHNRSWYIQATCATQGNGLYEGLDWLSKELEKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g14776.t2 | CDD | cd04150 | Arf1_5_like | 18 | 176 | 0.0000 |
| 9 | g14776.t2 | Gene3D | G3DSA:3.40.50.300 | - | 18 | 177 | 0.0000 |
| 2 | g14776.t2 | PANTHER | PTHR11711 | ADP RIBOSYLATION FACTOR-RELATED | 1 | 180 | 0.0000 |
| 3 | g14776.t2 | PANTHER | PTHR11711:SF386 | ADP-RIBOSYLATION FACTOR 5 | 1 | 180 | 0.0000 |
| 4 | g14776.t2 | PRINTS | PR00328 | GTP-binding SAR1 protein signature | 19 | 42 | 0.0000 |
| 7 | g14776.t2 | PRINTS | PR00328 | GTP-binding SAR1 protein signature | 47 | 71 | 0.0000 |
| 5 | g14776.t2 | PRINTS | PR00328 | GTP-binding SAR1 protein signature | 74 | 99 | 0.0000 |
| 6 | g14776.t2 | PRINTS | PR00328 | GTP-binding SAR1 protein signature | 119 | 140 | 0.0000 |
| 1 | g14776.t2 | Pfam | PF00025 | ADP-ribosylation factor family | 6 | 176 | 0.0000 |
| 15 | g14776.t2 | ProSiteProfiles | PS51417 | small GTPase Arf family profile. | 11 | 177 | 24.7550 |
| 11 | g14776.t2 | SMART | SM00177 | arf_sub_2 | 1 | 180 | 0.0000 |
| 13 | g14776.t2 | SMART | SM00178 | sar_sub_1 | 1 | 177 | 0.0000 |
| 12 | g14776.t2 | SMART | SM00175 | rab_sub_5 | 18 | 180 | 0.0032 |
| 8 | g14776.t2 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 14 | 177 | 0.0000 |
| 14 | g14776.t2 | TIGRFAM | TIGR00231 | small_GTP: small GTP-binding protein domain | 16 | 146 | 0.0000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005525 | GTP binding | MF |
KEGG
Orthology
- This transcript has been annotated with K07939 (ADP-ribosylation factor 4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
