General information for g14832.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Scoloptoxin SSD14.
Parent gene
- The parent gene g14832 is located on chr_4: 2580368..2584081 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14832 | g14832.t1 | isoform | g14832.t1 | 2580368 | 2584081 |
| chr_4 | g14832 | g14832.t1 | exon | g14832.t1.exon1 | 2580368 | 2580489 |
| chr_4 | g14832 | g14832.t1 | cds | g14832.t1.CDS1 | 2580368 | 2580489 |
| chr_4 | g14832 | g14832.t1 | exon | g14832.t1.exon2 | 2580545 | 2581216 |
| chr_4 | g14832 | g14832.t1 | cds | g14832.t1.CDS2 | 2580545 | 2581216 |
| chr_4 | g14832 | g14832.t1 | exon | g14832.t1.exon3 | 2582959 | 2584081 |
| chr_4 | g14832 | g14832.t1 | cds | g14832.t1.CDS3 | 2582959 | 2584081 |
| chr_4 | g14832 | g14832.t1 | TSS | g14832.t1 | NA | NA |
| chr_4 | g14832 | g14832.t1 | TTS | g14832.t1 | NA | NA |
Sequences
>g14832.t1 Gene=g14832 Length=1917
ATGAATACTCAATTACCAAAAAAATCTTCAAAATTTTTTAATACAAATATTTTACAATTT
TGCTTAACGATTTTCGGAGCAATTATTATATCTGCGGTTTATTATATTCATTCCACATCG
AGTTTAAACAAAGCAGCAGTTGTCACAAATGGTATTGAATGTGCGTCACTAGGTAGAAAA
ATATTTGATCTGGGTGGAAATGTTGCTGATGTTGCTGTGACTGTTATTTTATGTGAAGGC
GTGGCAAGTCCACAAAGTTCAGGAATTGGTGGTGGATTTTTCCTCAGTTATTATTCGAAA
TCTGAAGGAATTGTGAGAACTTTGAATGCTCGTGAAACTGCACCTGCTGCTGCAACGACT
GATATGTATGTTAATGACACAAATTCAGCAGTTTCTGGTGGAAAAGCAGTTGCTGTACCT
GGTGAAATTAAAGGACTTTGGGAATTGCATCAAAAATTTGGAAGTTTAGAATGGAAAATT
TTGTTGCAACCTGTGATTGAATTATGTAAAGAAGGTCATGTTGTGTCAAATTATCTGCAA
GATGTTTTTGCTCGTTATGAAGAAAGAATTTTCAATGAACCAAGTTTGCGAGAAATTTTT
ATTGATCCTGAAACAAATAAACTCTACAAAGAAGGAGAAAAAGTTAAAAGGTTGAAACTT
GCGGAAACCTTGGAAATTATTGCTAGAGATGGAGCGAATGCAATTTATGGAGGAGGTGAA
ATCGGAAGAATGTTAATTGAAGATGTACAGAAAATGAATGGAATTTTAACCGAAGAAGAT
TTGATGAATTATGAAGTAAAATGGTCAACATCATTAACATCAAAAATAATCAACAATCAA
ACCCTGCATACTTTTCCAATTCCAAGCAGTGGTGCAATTATAAATTTCATTTTTAATGTC
CTAAGCAATTATAAACTTTCTCACGATTCTCTTAGTTATCATCGAATTATTGAAGCTTTT
AAATTTGCTTATGCAAAACGAAGTTATTTAGGAGACGAAAATTCAACTAAAGTTCTTGAA
ATGATGGAAAATTTATTAAGTCAAAGTTATGCAAATGAAATTTCTTCTAAAATTAATGAT
ACTAAAACTTTCAACAATCCTGAACATTATGAAGCACTTTACGGAAACATAGAGGATAAA
GGAACAAGTCATATATCAATTTTAGCTCCAAACGGTGATGCAATTTCAGTCACAAGTACA
ATAAATTATTATTTTGGTGCAATTTTCAGAGCTCAACAAACAGGAATAATTTTGAATAAT
CAAATGGATGATTTTTCTACTCCAGGTATTTTGAATATTTATGAATATTTACCATCACCT
ATGAACTTCATTAAACCTGGAAAACGGGCATTATCATCGATGACTCCGACTTTCATAATT
GATGCAAAAGGAAATATTCGAATGATAGTTGGTGCTGAAGGAGGATCGAGAACTCCGACA
AGCATCTTTGCGTCTATTTTCAATCATCTTTATCTTAAAAAATCTTTAAAAGATGCAATT
GGAACAAGAAGACTTCATCATCAATTGTTTCCACCACAATTGCAATTTGAAACAAATTTT
GATGATGAAATTTTAAAAGATTTGAATGAAAATTTTGGTCATTTATTATTGGAAAATACA
CAAAATGATGATTTTGCTGCAGTGACTGTAATTGCTGTAAATAATGGCAAAATCGAAGCA
AGTTTTGATCCTAGACGTGGTGGAAGTGTTGAAATTTTTAGTGCATTATCGCAAATAAAT
ACAATATTAGGTTCACGATGGATGACTGCCTGCTTTATTGTCTGGTTAGGTATCCGTGTC
ATAACTCTAACTCGAAAACGCAAAACCATTGAAGGAAAGGGCCAATATAAGCTTATGTAT
ATGCTGCAAATGTGTACGATTCGGTTGAAAAAAAAATTTGACACCAAAAGTTTGTAA
>g14832.t1 Gene=g14832 Length=638
MNTQLPKKSSKFFNTNILQFCLTIFGAIIISAVYYIHSTSSLNKAAVVTNGIECASLGRK
IFDLGGNVADVAVTVILCEGVASPQSSGIGGGFFLSYYSKSEGIVRTLNARETAPAAATT
DMYVNDTNSAVSGGKAVAVPGEIKGLWELHQKFGSLEWKILLQPVIELCKEGHVVSNYLQ
DVFARYEERIFNEPSLREIFIDPETNKLYKEGEKVKRLKLAETLEIIARDGANAIYGGGE
IGRMLIEDVQKMNGILTEEDLMNYEVKWSTSLTSKIINNQTLHTFPIPSSGAIINFIFNV
LSNYKLSHDSLSYHRIIEAFKFAYAKRSYLGDENSTKVLEMMENLLSQSYANEISSKIND
TKTFNNPEHYEALYGNIEDKGTSHISILAPNGDAISVTSTINYYFGAIFRAQQTGIILNN
QMDDFSTPGILNIYEYLPSPMNFIKPGKRALSSMTPTFIIDAKGNIRMIVGAEGGSRTPT
SIFASIFNHLYLKKSLKDAIGTRRLHHQLFPPQLQFETNFDDEILKDLNENFGHLLLENT
QNDDFAAVTVIAVNNGKIEASFDPRRGGSVEIFSALSQINTILGSRWMTACFIVWLGIRV
ITLTRKRKTIEGKGQYKLMYMLQMCTIRLKKKFDTKSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g14832.t1 | Gene3D | G3DSA:1.10.246.130 | - | 272 | 374 | 6.9E-30 |
| 14 | g14832.t1 | Gene3D | G3DSA:3.60.20.40 | - | 377 | 573 | 1.9E-61 |
| 2 | g14832.t1 | PANTHER | PTHR11686 | GAMMA GLUTAMYL TRANSPEPTIDASE | 17 | 571 | 4.1E-210 |
| 3 | g14832.t1 | PANTHER | PTHR11686:SF51 | GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A | 17 | 571 | 4.1E-210 |
| 5 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 67 | 92 | 1.0E-62 |
| 8 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 136 | 154 | 1.0E-62 |
| 9 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 154 | 173 | 1.0E-62 |
| 10 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 255 | 271 | 1.0E-62 |
| 11 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 285 | 304 | 1.0E-62 |
| 12 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 382 | 400 | 1.0E-62 |
| 6 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 406 | 424 | 1.0E-62 |
| 7 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 446 | 461 | 1.0E-62 |
| 4 | g14832.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 471 | 488 | 1.0E-62 |
| 1 | g14832.t1 | Pfam | PF01019 | Gamma-glutamyltranspeptidase | 60 | 568 | 9.6E-161 |
| 16 | g14832.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 19 | g14832.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 36 | - |
| 18 | g14832.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 37 | 586 | - |
| 20 | g14832.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 587 | 604 | - |
| 17 | g14832.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 605 | 638 | - |
| 13 | g14832.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 35 | 570 | 1.18E-142 |
| 22 | g14832.t1 | TIGRFAM | TIGR00066 | g_glut_trans: gamma-glutamyltransferase | 48 | 565 | 7.0E-113 |
| 21 | g14832.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 34 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006751 | glutathione catabolic process | BP |
| GO:0036374 | glutathione hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K18592 (gamma-glutamyltranspeptidase / glutathione hydrolase / leukotriene-C4 hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed