General information for g14878.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamine–fructose-6-phosphate aminotransferase [isomerizing] 2.
Parent gene
- The parent gene g14878 is located on chr_4: 2811719..2812274 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14878 | g14878.t1 | isoform | g14878.t1 | 2801537 | 2811909 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon1 | 2801537 | 2801543 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS1 | 2801537 | 2801543 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon2 | 2806040 | 2806551 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS2 | 2806040 | 2806551 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon3 | 2806615 | 2806756 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS3 | 2806615 | 2806756 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon4 | 2810225 | 2810382 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS4 | 2810225 | 2810382 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon5 | 2810565 | 2811404 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS5 | 2810565 | 2811404 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon6 | 2811473 | 2811736 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS6 | 2811473 | 2811736 |
| chr_4 | g14878 | g14878.t1 | exon | g14878.t1.exon7 | 2811799 | 2811909 |
| chr_4 | g14878 | g14878.t1 | cds | g14878.t1.CDS7 | 2811799 | 2811909 |
| chr_4 | g14878 | g14878.t1 | TTS | g14878.t1 | 2812226 | 2812226 |
| chr_4 | g14878 | g14878.t1 | TSS | g14878.t1 | NA | NA |
Sequences
>g14878.t1 Gene=g14878 Length=2034
ATGTGTGGTATTTTTGCATACTTGAACTATCTAACACCAAAAACTCGTCGTGAGGTTCTC
GAATATCTTGTAACCGGCTTGAAGCGTCTCGAGTATCGTGGCTATGATTCGGCTGGTGTT
GCTGTGGATAGCGAGGATGGAAAAGGCATCGTGTTAGTAAAGCAAACTGGCAAGGTTAAA
GCACTTGAGGAGGCCATCAATGAAAGCATGATTGGCCATGACATCGACAGTCCATTGATG
TCTCATTGCGGTATCTCACATACACGTTGGGCTACTCATGGTGAACCAAGTGAGCTCAAT
TCACATCCACAACGAAGTGATGAAGAAAATAGCTTTGTTGTTGTTCACAATGGAATTATC
ACCAATTATAAGGATGTTAAAACTTTCTTGGAAAAACGTGGCTATAAATTTGAGTCTGAA
ACTGATACTGAAATCATTGCAAAACTTGTTCATCATTTATACATGCAACATCCAAATTAT
TCATTTAGAGAGTTGGTTGAACAAGTCATTCAGCAATTGGAAGGTGCATTTTCATTGGCC
TTCAAATCAAAACACTTTCCTGGTGAATGTGTCGCCACGCGACGTGGTTCACCACTCCTT
GTTGGAATAAAGACTAAAACACGTCTCGCAACTGATCATATTCCGATTTTGTATGGCAAA
GAACATCGTCCTCATGGCGCTAATCGTGAAGTTCCAATGATGCCAAGAACTGAAAGCACA
AGTGAATTTCAACCACTCGAAGATAAAGAAGTTGAATATTTCTTTGCATCTGATGCATCA
GCTGTTATTGAACATACAAATCGTGTCATCTATTTGGAAGACGATGACGTCGCAGCAGTC
AAAGAAGGCGCTCTCAGTATTCATCGCCTTAAAAAATCAATGGACGATTCACACGCTCGT
GAAATTATCACATTAAAAATGGAAATTCAACAAATTATGAAAGGAAATTACGAATATTTC
ATGCAAAAGGAAATTTTCGAACAACCTGAATCAGTGGTCAATACAATGCGTGGTCGTGTA
AATTTAAATGATAATACAGTAACACTTGGTGGAATTAAGGAATACATTCCAGAAATTAAG
CGTTGTCGTCGTCTTATGTTGATTGGTTGTGGAACATCATATCACAGTGCAGTTGCAACA
AGACAATTACTTGAAGAATTAACAGAATTGCCAGTTATGGTTGAACTTGCAAGTGATTTC
TTAGATCGAAACACACCAATTTTCCGTGATGACGTTTGTTTCTTTATTTCACAATCTGGT
GAAACTGCCGACACATTAATGGCATTGAGATATTGTAAACAACGTGGTGCATTGATTGTT
GGTATCACCAACACTGTCGGCAGTTCTATCTGTCGTGAATCACATTGTGGTGTGCATATT
AATGCTGGACCTGAAATTGGTGTTGCATCAACAAAAGCCTACACATCGCAATTCATTTCA
CTTGTTATGTTTGGTCTTGTTATGAGTGAAGATCGAATTTCATTGCAAACAAGAAGACAA
GAAATTTTAAACGGTTTAAAGAATTTGGATGCCCAAATTAAAGATGTATTGAAACTTGAT
TCACAAGTATTGGATATTGTTAAAGATTTATATCAACAAAAGAGTTTGCTGATTATGGGA
CGTGGTTATAATTTTGCTACATGTATGGAAGGTGCTTTGAAAGTGAAAGAATTGACATAC
ATGCATTGTGAAGGAATCATGGCTGGTGAACTTAAACATGGACCACTTGCTCTTGTTGAT
GATATGATGCCAATTGTGATGATTGTTATGCGTGATGGAGTTTATAATAAGACAATCAAT
GCCATACAACAAGTTAAAGCTAGAGGAGGTCAACCAATTGTCATTTGTGAAGAAAATGAC
AAGGAAACAATGGTGTTTGCCCATAAAGTTCTTGAAGTGCCGAAAACTGTTGATTGCCTT
CAAGGAATTTTGACTGTCATTCCAATGCAACTTCTTTCATACCACATTGCTGTCTTGCGT
GGTTGCAATGTTGATTGTCCAAGAAATCTCGCAAAATCTGTCACTGTGGAGTAA
>g14878.t1 Gene=g14878 Length=677
MCGIFAYLNYLTPKTRREVLEYLVTGLKRLEYRGYDSAGVAVDSEDGKGIVLVKQTGKVK
ALEEAINESMIGHDIDSPLMSHCGISHTRWATHGEPSELNSHPQRSDEENSFVVVHNGII
TNYKDVKTFLEKRGYKFESETDTEIIAKLVHHLYMQHPNYSFRELVEQVIQQLEGAFSLA
FKSKHFPGECVATRRGSPLLVGIKTKTRLATDHIPILYGKEHRPHGANREVPMMPRTEST
SEFQPLEDKEVEYFFASDASAVIEHTNRVIYLEDDDVAAVKEGALSIHRLKKSMDDSHAR
EIITLKMEIQQIMKGNYEYFMQKEIFEQPESVVNTMRGRVNLNDNTVTLGGIKEYIPEIK
RCRRLMLIGCGTSYHSAVATRQLLEELTELPVMVELASDFLDRNTPIFRDDVCFFISQSG
ETADTLMALRYCKQRGALIVGITNTVGSSICRESHCGVHINAGPEIGVASTKAYTSQFIS
LVMFGLVMSEDRISLQTRRQEILNGLKNLDAQIKDVLKLDSQVLDIVKDLYQQKSLLIMG
RGYNFATCMEGALKVKELTYMHCEGIMAGELKHGPLALVDDMMPIVMIVMRDGVYNKTIN
AIQQVKARGGQPIVICEENDKETMVFAHKVLEVPKTVDCLQGILTVIPMQLLSYHIAVLR
GCNVDCPRNLAKSVTVE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g14878.t1 | CDD | cd00714 | GFAT | 2 | 280 | 0.000 |
| 15 | g14878.t1 | CDD | cd05008 | SIS_GlmS_GlmD_1 | 364 | 489 | 0.000 |
| 14 | g14878.t1 | CDD | cd05009 | SIS_GlmS_GlmD_2 | 522 | 675 | 0.000 |
| 8 | g14878.t1 | Gene3D | G3DSA:3.40.50.10490 | - | 315 | 676 | 0.000 |
| 9 | g14878.t1 | Gene3D | G3DSA:3.40.50.10490 | - | 515 | 662 | 0.000 |
| 4 | g14878.t1 | PANTHER | PTHR10937:SF10 | GLUTAMINE–FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE [ISOMERIZING] 2 | 1 | 677 | 0.000 |
| 5 | g14878.t1 | PANTHER | PTHR10937 | GLUCOSAMINE–FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING | 1 | 677 | 0.000 |
| 3 | g14878.t1 | Pfam | PF13522 | Glutamine amidotransferase domain | 81 | 191 | 0.000 |
| 2 | g14878.t1 | Pfam | PF01380 | SIS domain | 359 | 484 | 0.000 |
| 1 | g14878.t1 | Pfam | PF01380 | SIS domain | 530 | 659 | 0.000 |
| 13 | g14878.t1 | ProSiteProfiles | PS51278 | Glutamine amidotransferase type 2 domain profile. | 2 | 283 | 39.852 |
| 11 | g14878.t1 | ProSiteProfiles | PS51464 | SIS domain profile. | 355 | 494 | 27.808 |
| 12 | g14878.t1 | ProSiteProfiles | PS51464 | SIS domain profile. | 526 | 667 | 24.430 |
| 6 | g14878.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 2 | 289 | 0.000 |
| 7 | g14878.t1 | SUPERFAMILY | SSF53697 | SIS domain | 314 | 677 | 0.000 |
| 10 | g14878.t1 | TIGRFAM | TIGR01135 | glmS: glutamine-fructose-6-phosphate transaminase (isomerizing) | 251 | 677 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1901135 | carbohydrate derivative metabolic process | BP |
| GO:0097367 | carbohydrate derivative binding | MF |
| GO:1901137 | carbohydrate derivative biosynthetic process | BP |
| GO:0004360 | glutamine-fructose-6-phosphate transaminase (isomerizing) activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00820 (glutamine—fructose-6-phosphate transaminase (isomerizing))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
