Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Chymotrypsin-2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15013 g15013.t23 TSS g15013.t23 3513859 3513859
chr_4 g15013 g15013.t23 isoform g15013.t23 3513904 3515116
chr_4 g15013 g15013.t23 exon g15013.t23.exon1 3513904 3514477
chr_4 g15013 g15013.t23 cds g15013.t23.CDS1 3513904 3514477
chr_4 g15013 g15013.t23 exon g15013.t23.exon2 3514600 3514687
chr_4 g15013 g15013.t23 cds g15013.t23.CDS2 3514600 3514613
chr_4 g15013 g15013.t23 exon g15013.t23.exon3 3515087 3515116
chr_4 g15013 g15013.t23 TTS g15013.t23 3515192 3515192

Sequences

>g15013.t23 Gene=g15013 Length=692
ATGTTTAAATTTATCGTTCTTTTGGGCCTTGTGTCATCAGCACTCTGTGCCAGTCTTTCA
TCACCTGCCATTGATCCAGAATGGGAAGGTTTCATTGTAGGAGGAAGCACAGCTTCAGCT
GGTCAATTCCCACATCAAGTTTCACTTAGATCATCAGGAAATGCTCATTTCTGTGGTGGT
TTCATTATTAACAATCGCTGGGTTGGATCAGCTGCTCACTGCACAGTTGGACGTACTGCC
GCAAACACAATCTCAGTTGTTGGTACCAACAGTCGTACATCAGGTGGTGTTGCTCACAGT
ACATCACGCATTGTCAACCATCCAAGTTACAATGGCAACACATTGGCCAATGATATTTCA
ATGGTTTTCACTGCATCAACAATTACCTTCAATAACTTGGTTCAACCAATCACTCTTGGA
TCAGCTCATGTTGGCGGTGGTGTTTCAGCAACTGCATCAGGTTGGGGTCAAACATCACAT
CCAGGCTCAGCTGCTGCCAATTTGCAATTCTTAACTAAACCAACTTTGACAAATGCTGAC
TGCCGTGCTCGTCACTCTGCTGGAAATGCTGCCCTGGTGGCCCATTGATTGCTGGTGGTA
CAGTTATTGGTGCAGTTTCATGGGGTATTGCATGCGCTCAAGGATTCCCTGATGTTTTCG
CTCATCGCACATGGATGATCAACCAAATGTAA

>g15013.t23 Gene=g15013 Length=195
MFKFIVLLGLVSSALCASLSSPAIDPEWEGFIVGGSTASAGQFPHQVSLRSSGNAHFCGG
FIINNRWVGSAAHCTVGRTAANTISVVGTNSRTSGGVAHSTSRIVNHPSYNGNTLANDIS
MVFTASTITFNNLVQPITLGSAHVGGGVSATASGWGQTSHPGSAAANLQFLTKPTLTNAD
CRARHSAGNAALVAH

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g15013.t23 CDD cd00190 Tryp_SPc 32 190 3.39525E-47
8 g15013.t23 Gene3D G3DSA:2.40.10.10 - 32 187 6.2E-37
7 g15013.t23 Gene3D G3DSA:2.40.10.10 - 44 136 6.2E-37
2 g15013.t23 PANTHER PTHR24276 POLYSERASE-RELATED 30 185 3.2E-35
3 g15013.t23 PANTHER PTHR24276:SF89 IP07603P 30 185 3.2E-35
1 g15013.t23 Pfam PF00089 Trypsin 32 185 1.4E-31
10 g15013.t23 Phobius SIGNAL_PEPTIDE Signal peptide region 1 16 -
11 g15013.t23 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 3 -
12 g15013.t23 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 4 11 -
13 g15013.t23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 12 16 -
9 g15013.t23 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 17 195 -
17 g15013.t23 ProSiteProfiles PS50240 Serine proteases, trypsin domain profile. 32 195 17.654
16 g15013.t23 SMART SM00020 trypsin_2 31 194 3.8E-15
4 g15013.t23 SUPERFAMILY SSF50494 Trypsin-like serine proteases 5 186 1.05E-43
6 g15013.t23 SignalP_EUK SignalP-noTM SignalP-noTM 1 16 -
15 g15013.t23 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM 1 23 -
5 g15013.t23 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM 1 23 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004252 serine-type endopeptidase activity MF
GO:0006508 proteolysis BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed