General information for g15013.t28
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-2.
Parent gene
- The parent gene g15013 is located on chr_4: 3513904..3515591 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15013 | g15013.t28 | TSS | g15013.t28 | 3513859 | 3513859 |
| chr_4 | g15013 | g15013.t28 | isoform | g15013.t28 | 3513904 | 3515116 |
| chr_4 | g15013 | g15013.t28 | exon | g15013.t28.exon1 | 3513904 | 3514534 |
| chr_4 | g15013 | g15013.t28 | cds | g15013.t28.CDS1 | 3513904 | 3514534 |
| chr_4 | g15013 | g15013.t28 | exon | g15013.t28.exon2 | 3514594 | 3514700 |
| chr_4 | g15013 | g15013.t28 | cds | g15013.t28.CDS2 | 3514594 | 3514613 |
| chr_4 | g15013 | g15013.t28 | exon | g15013.t28.exon3 | 3515087 | 3515116 |
| chr_4 | g15013 | g15013.t28 | TTS | g15013.t28 | 3515192 | 3515192 |
Sequences
>g15013.t28 Gene=g15013 Length=768
ATGTTTAAATTTATCGTTCTTTTGGGCCTTGTGTCATCAGCACTCTGTGCCAGTCTTTCA
TCACCTGCCATTGATCCAGAATGGGAAGGTTTCATTGTAGGAGGAAGCACAGCTTCAGCT
GGTCAATTCCCACATCAAGTTTCACTTAGATCATCAGGAAATGCTCATTTCTGTGGTGGT
TTCATTATTAACAATCGCTGGGTTGGATCAGCTGCTCACTGCACAGTTGGACGTACTGCC
GCAAACACAATCTCAGTTGTTGGTACCAACAGTCGTACATCAGGTGGTGTTGCTCACAGT
ACATCACGCATTGTCAACCATCCAAGTTACAATGGCAACACATTGGCCAATGATATTTCA
ATGGTTTTCACTGCATCAACAATTACCTTCAATAACTTGGTTCAACCAATCACTCTTGGA
TCAGCTCATGTTGGCGGTGGTGTTTCAGCAACTGCATCAGGTTGGGGTCAAACATCACAT
CCAGGCTCAGCTGCTGCCAATTTGCAATTCTTAACTAAACCAACTTTGACAAATGCTGAC
TGCCGTGCTCGTCACTCTGCTGGAAATGCTGCCCGTGTCTTTGACAACACTATCTGTACA
TTCTTGAGAGCAGGAGCTGGTATGTGCATGGAACTAGTGGTGGCCCATTGATTGCTGGTG
GTACAGTTATTGGTGCAGTTTCATGGGGTATTGCATGCGCTCAAGGATTCCCTGATGTTT
TCGCTGTAATGACTCAAACATCGCACATGGATGATCAACCAAATGTAA
>g15013.t28 Gene=g15013 Length=216
MFKFIVLLGLVSSALCASLSSPAIDPEWEGFIVGGSTASAGQFPHQVSLRSSGNAHFCGG
FIINNRWVGSAAHCTVGRTAANTISVVGTNSRTSGGVAHSTSRIVNHPSYNGNTLANDIS
MVFTASTITFNNLVQPITLGSAHVGGGVSATASGWGQTSHPGSAAANLQFLTKPTLTNAD
CRARHSAGNAARVFDNTICTFLRAGAGMCMELVVAH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g15013.t28 | CDD | cd00190 | Tryp_SPc | 32 | 210 | 4.62555E-50 |
| 8 | g15013.t28 | Gene3D | G3DSA:2.40.10.10 | - | 32 | 209 | 7.6E-39 |
| 7 | g15013.t28 | Gene3D | G3DSA:2.40.10.10 | - | 44 | 136 | 7.6E-39 |
| 2 | g15013.t28 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 30 | 209 | 5.2E-37 |
| 3 | g15013.t28 | PANTHER | PTHR24276:SF89 | IP07603P | 30 | 209 | 5.2E-37 |
| 1 | g15013.t28 | Pfam | PF00089 | Trypsin | 32 | 202 | 1.1E-33 |
| 10 | g15013.t28 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 11 | g15013.t28 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g15013.t28 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 11 | - |
| 13 | g15013.t28 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 9 | g15013.t28 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 216 | - |
| 17 | g15013.t28 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 32 | 209 | 18.816 |
| 16 | g15013.t28 | SMART | SM00020 | trypsin_2 | 31 | 213 | 3.6E-23 |
| 4 | g15013.t28 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 5 | 209 | 3.24E-46 |
| 6 | g15013.t28 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 15 | g15013.t28 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 5 | g15013.t28 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 23 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
