General information for g1502.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphoinositide 3-kinase regulatory subunit 4.
Parent gene
- The parent gene g1502 is located on chr_3: 11429370..11430307 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1502 | g1502.t1 | TTS | g1502.t1 | 11424781 | 11424781 |
| chr_3 | g1502 | g1502.t1 | isoform | g1502.t1 | 11424912 | 11429907 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon1 | 11424912 | 11425146 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS1 | 11424912 | 11425146 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon2 | 11425208 | 11426715 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS2 | 11425208 | 11426715 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon3 | 11426772 | 11427379 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS3 | 11426772 | 11427379 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon4 | 11427433 | 11427841 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS4 | 11427433 | 11427841 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon5 | 11428631 | 11429578 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS5 | 11428631 | 11429578 |
| chr_3 | g1502 | g1502.t1 | exon | g1502.t1.exon6 | 11429635 | 11429907 |
| chr_3 | g1502 | g1502.t1 | cds | g1502.t1.CDS6 | 11429635 | 11429907 |
| chr_3 | g1502 | g1502.t1 | TSS | g1502.t1 | 11430138 | 11430138 |
Sequences
>g1502.t1 Gene=g1502 Length=3981
ATGGGAAATCAATTGTCAATAGCTCCCTCGCAAATATTTCCTGTAGAGCATTATCTGACT
GGATTGGAGAATGAGATACAGTTTGAATCCAATTTAGGTAGTACACGATTTTTCAAAGTT
GCAAGAGCAAAATCTGAAGAAGGCGAGTTGGTTGTGAAAGTTTTCGTAAAACATGATCCT
ACTTTGCCTCTTGAAGTTCATGCTGAGCTTATTGAAACAATTAAGAAGAAATTACAAAAT
GCAGTAAATTGTTTGCCATTTCAAAAAGTTGTGTCATCAGATCGAGCAGGAATTATTATA
AGAGAATATGTAAAGCATAGTCTTTATGATCGAATGTCAACAAGACCATTCCTTACACTT
ATTGAGAAGAAATGGATAACATTTCAAATGCTTTGTGCTCTTCATCAATGTCATAAGCAA
AAAGTTTGTCATGGTGATATAAAATTAGAAAATATTCTAATTACTTCATGGAACTGGATT
TTACTTTCTGATTTTGCATCATTCAAACCAACATATTTACCAGAAGATAATCCAGCGGAT
TACAGTTACTTTTTTGATACAAGTCGCAAAAGAACTTGTTATATTGCTCCCGAGAGATTT
GTCAAAAGCTTAGCATCGGAAGTTAAAGAAAACAATCCATTAGTTGGCGACAATACCTGT
AATTCGGGAAATCTACTGCCTGAAATGGATATCTTCTCAGCTGGATGTGCTTTATTAGAA
TTGTGGACAGAAGAAACAGCACCATTTGAATTTTTTCAATTACTTACCTATCGAAGTGGT
AAAAACGATTTAGTTGTGAAACATTTAAACAGCATTCAAGATGAAAATTTAAAAAGTTTA
CTTTCATCAATGCTCAGTATAAATCCGAAAGAGAGAAAATCAGCAGAAATTTATCTTGAT
TTAGAACGAGGTCGACTATTTCCTGAATATTTTTATTCATTTCTTCAATCTTATATACCA
ATCACATTTAGCTCTATTCCAATTGTTCCTAATGATGACAAGATATTGAGGCTTCATTCT
GATATTACACAAATTATTGATATAATAACTAAGGACAAACCAGAAGATGATGATGGATTA
ATTTTGATAACGACTCTTGTAACTTCTTGCATACGTGGACTCATACATTGCTCATCAAAA
ATTAAATGTTTGGAAATTCTTCACAAACTCGCACATCATACCTCATCTGAGACAATTTTA
GATAGAATTTTACCTTATATTGTGCATTTATGTCAAGATGCATCTGCAAATGTTCGAGTT
GCTGCACTTGATACACTCACACAATGTCTTTGTTTAGTAAAAAATCTTCCCCGATCAGAT
GCAAATGTTTTTCCTGAATATGTTTTACCATCAATTGCACCATTAGCGGTTGATGCAGCA
ACAATTGTGAGAGTAGCTTATGCTCAAAATATTGCAACATTAGCTGAAACAGCTGTGAGA
TTTTTAGAACAATCACAATTATTGACAAATGAAGGACCAGTACCAAATTATGATAATGAA
CTTCATGACCTTCAAGAAATGCTTTCGACAACTGTAATGAATTTATTGACCGATTCACAT
GTTGTTGTTAAACAAACTCTTATGGAATCTGGAATAACTAAACTATGTGTATTTTTTGGC
AGTCAAAAGGCAAACGACATTATTCTCTCTCATGTCATCACATTCCTCAATTCACTCAAT
GATCGAGATGATAAAAGCTTACGCGGAACATTTTTTGATTGCATCGTTGGCATTGCAACA
TATGTTGGATGGCATTGTTCTCCAATTTTAATTCCATTACTTCAACAAGGTTTCAGTGAC
CCAGAAGAATTTATAATTTCAAAAGCGATTCGTGCAACAACAGCACTCGCTGAATTAGGA
TTGCTTCAAAAGACGAGCTTAATCGATTTTGTTCACGAATGTGCATGCTTTCTCAATCAT
CCAAATTTATGGATTCGACATGAAATATGTGGTCTAATATCGACTGCTGCAAAAATGCTT
ACACCATTGGATGTACAGTGCAAAATTATGCCATCGGTTGTACCTCATTTGAAAAGTCCA
TTAATACAATTGGAGCGCGTAGAATTGCTACATGATTCTATTCATACGCCAATTCCTAGA
GACGTTTATGAAACTGTGTTGAAATTTCCAGATATTGTTTCATTAATGGATGTATTGAAA
GAAAGAAAAAAAGCAAGAGCTAAAGCAGGAGCTGATGGAATACCAAAATATGGTGAAATG
GCTCAATCAATGAGAACTCTATTTCGGCGTCTATCATCAATTGAAGGATGGAGTGATTTA
ACTGAAGTTCAAATTCTATCAATGAGTCATCATTTATTGAAAATTGCACGTTATAAGGCT
CATGAAAATCGAAATCTACGCGATGGTCGTATACAATTGGATAAAAATCAAGCAAGTTTA
CTACATGAAGTGATTCTCAGTAATGTTGGCATAGATAAAATGACAAAAAATGATTTAACA
AATCTCGTTGGTGGTAGAAAGCATCGAATAAGTGAATCAACAAGTGCAGATTTACTTCTC
TATCATGATTTTCATACACAATCACCAATGCCTGACACAATTTCACTCAATTCACAGCCT
ATTGTTCATACAGCTACACCAACATTGTCTCTTGTCGAGTACAGTATGCCTGAGCGTAGT
TTTCATCAAGAACGTTTGTCAGATTGTCGTATTGAATTAGATCAACTATGCCAGCAATTA
AAGACGAAATTTACAAAAAATTCAAATCGTAGTGATACTTTGTTGAATGAAAATCAAGCA
ACAATCCAATTAGCACCAAATTGGAAATTCAATGGCACTTTGGTTGCACATTTGCATGAA
CATCGTAGTGCTGTAACAAGAATGACAAGTCTTAAACCAGGTGGAAGTGTTTTTGCAAGT
GCAAGCAGTGATGGAACTGTGAGATTATGGGATTGTACGAAGCTCGATGGTCATCAAAAC
ATTAATCGTTCACGACAATATTATGCAGCAAATACTCCATTAAATGCATTAGCAGCATGT
GATAATGGTCAATCGTTGGCAATTGCTGGACGTGATGGTGCATTATTAGTGTTGAGAATT
GATCCAAACTCGAGTAAAATGGCTCTTCAGCAAGCACGTCATTTAGATTCGAGCACGAAA
ATTGATAGAACAGTACACGAACAAGATGATGGGCCAGTTGTTGAGATGCAAACCAAAGAC
TACAGCCAAATCGCTTATGCAACTCTCTATGGTGCAATTGTCGGTTGGGATCTTCGAATG
CCAGCTTCTCAAAATGCATGGCGACTTGAGAGTGACTTAAGAAATGGTGTGATTACAACA
TTCACAATTGATCCATTAAATTCATCATGGTTAGCAATTGGAACAAGCAGTGGTCGTCAT
TTATGTTGGGATCTCAGATTTTCACTAAAAATTGCAGAAATTAAGCATCCATATGAATCG
AGAATTAGAAGAGTGGCATGCCATCCTACAGAGCCTTCATGTTTAATCTCTGCATCACAA
GGCAATAATGAAGTGTTTTCATGGAATATTGAAACAAACTCGAGGCAAACAGCTCTATGG
GCGAGCACAGCACCGCCATTATCGAACATTAATGCAACGACACATTCAGTTTGTGCACTT
TTGCCTGGCTCAGTAGACGGACGAGGATTTTTAATCACAGGTGGAACTGACCAACGAATT
CGTTATTGGGATTTACAAGATGCATCAAATTGCCGTTTAGTTGTTTCTGCTCCTAAAGAT
TGTTCTCCATCGAATGTCACATATGACTCTCGTTTAATTGATGGCACATATGTTATTCAA
GAAACACAAAATGTAAATCAATCTCCAACTCATAATGCTACTGCACAACGTGGTAGTGTA
GATGATACAATTAAATTCGGACCAGAACTACCACCACCCGGACATCATGATGTAATCACT
GATTTAGTTATGTGTAAAACGCAAAAGCAAACATTCGTGGCGTCGAGTAGTCGCGATGGT
GTCATTAAATTATGGAAATAA
>g1502.t1 Gene=g1502 Length=1326
MGNQLSIAPSQIFPVEHYLTGLENEIQFESNLGSTRFFKVARAKSEEGELVVKVFVKHDP
TLPLEVHAELIETIKKKLQNAVNCLPFQKVVSSDRAGIIIREYVKHSLYDRMSTRPFLTL
IEKKWITFQMLCALHQCHKQKVCHGDIKLENILITSWNWILLSDFASFKPTYLPEDNPAD
YSYFFDTSRKRTCYIAPERFVKSLASEVKENNPLVGDNTCNSGNLLPEMDIFSAGCALLE
LWTEETAPFEFFQLLTYRSGKNDLVVKHLNSIQDENLKSLLSSMLSINPKERKSAEIYLD
LERGRLFPEYFYSFLQSYIPITFSSIPIVPNDDKILRLHSDITQIIDIITKDKPEDDDGL
ILITTLVTSCIRGLIHCSSKIKCLEILHKLAHHTSSETILDRILPYIVHLCQDASANVRV
AALDTLTQCLCLVKNLPRSDANVFPEYVLPSIAPLAVDAATIVRVAYAQNIATLAETAVR
FLEQSQLLTNEGPVPNYDNELHDLQEMLSTTVMNLLTDSHVVVKQTLMESGITKLCVFFG
SQKANDIILSHVITFLNSLNDRDDKSLRGTFFDCIVGIATYVGWHCSPILIPLLQQGFSD
PEEFIISKAIRATTALAELGLLQKTSLIDFVHECACFLNHPNLWIRHEICGLISTAAKML
TPLDVQCKIMPSVVPHLKSPLIQLERVELLHDSIHTPIPRDVYETVLKFPDIVSLMDVLK
ERKKARAKAGADGIPKYGEMAQSMRTLFRRLSSIEGWSDLTEVQILSMSHHLLKIARYKA
HENRNLRDGRIQLDKNQASLLHEVILSNVGIDKMTKNDLTNLVGGRKHRISESTSADLLL
YHDFHTQSPMPDTISLNSQPIVHTATPTLSLVEYSMPERSFHQERLSDCRIELDQLCQQL
KTKFTKNSNRSDTLLNENQATIQLAPNWKFNGTLVAHLHEHRSAVTRMTSLKPGGSVFAS
ASSDGTVRLWDCTKLDGHQNINRSRQYYAANTPLNALAACDNGQSLAIAGRDGALLVLRI
DPNSSKMALQQARHLDSSTKIDRTVHEQDDGPVVEMQTKDYSQIAYATLYGAIVGWDLRM
PASQNAWRLESDLRNGVITTFTIDPLNSSWLAIGTSSGRHLCWDLRFSLKIAEIKHPYES
RIRRVACHPTEPSCLISASQGNNEVFSWNIETNSRQTALWASTAPPLSNINATTHSVCAL
LPGSVDGRGFLITGGTDQRIRYWDLQDASNCRLVVSAPKDCSPSNVTYDSRLIDGTYVIQ
ETQNVNQSPTHNATAQRGSVDDTIKFGPELPPPGHHDVITDLVMCKTQKQTFVASSSRDG
VIKLWK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g1502.t1 | CDD | cd13980 | STKc_Vps15 | 25 | 311 | 1.6095E-145 |
| 14 | g1502.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 19 | 304 | 1.2E-27 |
| 13 | g1502.t1 | Gene3D | G3DSA:1.25.10.10 | - | 363 | 489 | 6.0E-9 |
| 12 | g1502.t1 | Gene3D | G3DSA:1.25.10.10 | - | 497 | 717 | 4.4E-6 |
| 11 | g1502.t1 | Gene3D | G3DSA:2.130.10.10 | - | 892 | 1326 | 1.4E-81 |
| 4 | g1502.t1 | PANTHER | PTHR17583 | PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4 | 1 | 1326 | 0.0 |
| 7 | g1502.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 958 | 972 | 2.1E-5 |
| 5 | g1502.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 1211 | 1225 | 2.1E-5 |
| 6 | g1502.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 1313 | 1326 | 2.1E-5 |
| 3 | g1502.t1 | Pfam | PF00069 | Protein kinase domain | 29 | 296 | 5.9E-20 |
| 2 | g1502.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 933 | 971 | 0.0012 |
| 1 | g1502.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 1293 | 1326 | 0.16 |
| 16 | g1502.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 142 | 154 | - |
| 30 | g1502.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 26 | 315 | 20.047 |
| 29 | g1502.t1 | ProSiteProfiles | PS50077 | HEAT repeat profile. | 403 | 426 | 8.522 |
| 25 | g1502.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 938 | 1028 | 9.889 |
| 27 | g1502.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 938 | 971 | 11.043 |
| 26 | g1502.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 1210 | 1233 | 8.871 |
| 24 | g1502.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 1292 | 1326 | 9.599 |
| 28 | g1502.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 1292 | 1326 | 9.807 |
| 17 | g1502.t1 | SMART | SM00220 | serkin_6 | 26 | 311 | 6.4E-11 |
| 19 | g1502.t1 | SMART | SM00320 | WD40_4 | 931 | 971 | 7.2E-7 |
| 22 | g1502.t1 | SMART | SM00320 | WD40_4 | 980 | 1019 | 40.0 |
| 21 | g1502.t1 | SMART | SM00320 | WD40_4 | 1086 | 1124 | 110.0 |
| 23 | g1502.t1 | SMART | SM00320 | WD40_4 | 1127 | 1169 | 19.0 |
| 20 | g1502.t1 | SMART | SM00320 | WD40_4 | 1183 | 1224 | 1.8 |
| 18 | g1502.t1 | SMART | SM00320 | WD40_4 | 1283 | 1326 | 0.084 |
| 9 | g1502.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 24 | 301 | 8.06E-35 |
| 8 | g1502.t1 | SUPERFAMILY | SSF48371 | ARM repeat | 270 | 683 | 7.4E-32 |
| 10 | g1502.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 928 | 1326 | 8.77E-35 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0005515 | protein binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08333 (phosphoinositide-3-kinase, regulatory subunit 4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
