General information for g15028.t15
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g15028 is located on chr_4: 3588891..3590717 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15028 | g15028.t15 | TTS | g15028.t15 | 3587251 | 3587251 |
| chr_4 | g15028 | g15028.t15 | isoform | g15028.t15 | 3587549 | 3590717 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon1 | 3587549 | 3587768 |
| chr_4 | g15028 | g15028.t15 | cds | g15028.t15.CDS1 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon2 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t15 | cds | g15028.t15.CDS2 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon3 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t15 | cds | g15028.t15.CDS3 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon4 | 3588700 | 3588752 |
| chr_4 | g15028 | g15028.t15 | cds | g15028.t15.CDS4 | 3588700 | 3588752 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon5 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t15 | cds | g15028.t15.CDS5 | 3588818 | 3588950 |
| chr_4 | g15028 | g15028.t15 | exon | g15028.t15.exon6 | 3590537 | 3590717 |
| chr_4 | g15028 | g15028.t15 | TSS | g15028.t15 | 3590713 | 3590713 |
Sequences
>g15028.t15 Gene=g15028 Length=1390
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGGTT
GAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCAAATTTCAATGATTTTAGTG
GTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGCATCAGATTGCTACTAGAAA
ATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGGCTAATTGTACTGAGTGGAA
AAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCTTTATATTCAAGTGTGCCAA
TTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGTGGAGATTATTCCAGAAACAACAG
GCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTTTTGCCAATCGTTGTAATTA
ATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTTGTCTGTTTTTTGTACTGTA
TACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTATGGTGTCGTCAAAATCAAA
AGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTTAGTATAGCAGAATTTG
AAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAATTTTATTGCTAATGATA
CAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGATAATTTAAATCTTCGTGATC
TTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTAAATTTCCCAAGAACAACTC
GAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTAGTTGTCGGAGTTAATATTG
TCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAAAATTCTCTTATTCCTTTCT
CAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAGCTTGCTATTCCAAATCATT
TTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACCCTTAATCTTCTTGGTGAAA
TTTTACATTTTGCTGATCGCAATTTTTACTGTGACTGGTGGAACGCAAATAACGTTGATA
CATTTTGGAAAACATGGAATATGCCTGTCCATCGTTGGTGTGTGAGACATTTATATATTC
CACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTCTGGAGTTGTTTTTTTCATTAGTG
CCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCGAACATTTAAAATTTGGGCTTTTA
TGGGAATGGT
>g15028.t15 Gene=g15028 Length=372
MGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSSVPIVLCIIIEKGL
SVEIIPETTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFLYCILFVKLWSYVQ
VNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIANDTKDHEPLKLIE
YPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIKRILEVVVGVNIVMALFQQWMIP
SVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLLGEILHFADRNFYC
DWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSRFAASGVVFFISAFFHEYLVSVP
LRTFKIWAFMGM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g15028.t15 | PANTHER | PTHR10408:SF7 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 4 | 372 | 9.7E-121 |
| 3 | g15028.t15 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 4 | 372 | 9.7E-121 |
| 23 | g15028.t15 | PIRSF | PIRSF000439 | Oat_ACAT_DAG_ARE | 1 | 372 | 2.7E-112 |
| 1 | g15028.t15 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 94 | 370 | 3.0E-31 |
| 14 | g15028.t15 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 39 | - |
| 20 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 40 | 60 | - |
| 10 | g15028.t15 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 61 | 71 | - |
| 22 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 72 | 91 | - |
| 16 | g15028.t15 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 92 | 96 | - |
| 18 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 97 | 119 | - |
| 11 | g15028.t15 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 120 | 219 | - |
| 17 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 220 | 239 | - |
| 15 | g15028.t15 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 240 | 269 | - |
| 21 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 270 | 288 | - |
| 12 | g15028.t15 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 289 | 334 | - |
| 19 | g15028.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 335 | 352 | - |
| 13 | g15028.t15 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 353 | 372 | - |
| 8 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 40 | 62 | - |
| 9 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 74 | 91 | - |
| 7 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 118 | - |
| 5 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 220 | 239 | - |
| 6 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 269 | 291 | - |
| 4 | g15028.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 335 | 357 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004144 | diacylglycerol O-acyltransferase activity | MF |
| GO:0019432 | triglyceride biosynthetic process | BP |
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00637 (sterol O-acyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed