General information for g15028.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Diacylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g15028 is located on chr_4: 3588891..3590717 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15028 | g15028.t3 | TTS | g15028.t3 | 3587251 | 3587251 |
| chr_4 | g15028 | g15028.t3 | isoform | g15028.t3 | 3587302 | 3588354 |
| chr_4 | g15028 | g15028.t3 | exon | g15028.t3.exon1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t3 | cds | g15028.t3.CDS1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t3 | exon | g15028.t3.exon2 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t3 | cds | g15028.t3.CDS2 | 3587551 | 3587718 |
| chr_4 | g15028 | g15028.t3 | exon | g15028.t3.exon3 | 3587838 | 3587936 |
| chr_4 | g15028 | g15028.t3 | exon | g15028.t3.exon4 | 3588197 | 3588354 |
| chr_4 | g15028 | g15028.t3 | TSS | g15028.t3 | NA | NA |
Sequences
>g15028.t3 Gene=g15028 Length=661
AATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTTAGTATAGCA
GAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAATTTTATTGCT
AATGATACAAAAGACCATGAACCACTGAAATTGATAGACAAATCATTTTATATGGCTGTG
TGGGTTCTATCTAATTTTTCATTCAACCCTTAATCTTCTTGGTGAAATTTTACATTTTGC
TGATCGCAATTTTTACTGTGACTGGTGGAACGCAAATAACGTTGATACATTTTGGAAAAC
ATGGAATATGCCTGTCCATCGTTGGTGTGTGAGACATTTATATATTCCACTTGTTGATAT
GGGATATAGTCGTTTTGCTGCTTCTGGAGTTGTTTTTTTCATTAGTGCCTTCTTCCACGA
ATATTTGGTATCAGTTCCTCTTCGAACATTTAAAATTTGGGCTTTTATGGGAATGGTTTC
GCAGTTACCTCTTTCTTATGTATCAAAATTTCTCGAAAAAAATGTAGGAGAAAGATGGGG
AAATATTTTAGTATGGGGAAGTTTAATTTTAGGGCAACCACTGTGTATCATGGTCTATTA
TCATGACTATGTCATCCAAAATTATAATAAAGTTATACTTGAACCTGAATATTTAGAATA
G
>g15028.t3 Gene=g15028 Length=117
MPVHRWCVRHLYIPLVDMGYSRFAASGVVFFISAFFHEYLVSVPLRTFKIWAFMGMVSQL
PLSYVSKFLEKNVGERWGNILVWGSLILGQPLCIMVYYHDYVIQNYNKVILEPEYLE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g15028.t3 | PANTHER | PTHR10408:SF7 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 1 | 105 | 1.1E-40 |
| 3 | g15028.t3 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 1 | 105 | 1.1E-40 |
| 1 | g15028.t3 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 1 | 97 | 3.4E-10 |
| 8 | g15028.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 19 | - |
| 11 | g15028.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 41 | - |
| 6 | g15028.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 42 | 47 | - |
| 12 | g15028.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 48 | 65 | - |
| 9 | g15028.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 66 | 76 | - |
| 10 | g15028.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 77 | 98 | - |
| 7 | g15028.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 99 | 117 | - |
| 4 | g15028.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 23 | 45 | - |
| 5 | g15028.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 77 | 99 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004144 | diacylglycerol O-acyltransferase activity | MF |
| GO:0019432 | triglyceride biosynthetic process | BP |
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
