Gene loci information

Transcript annotation

  • This transcript has been annotated as Diacylglycerol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15028 g15028.t4 TTS g15028.t4 3587251 3587251
chr_4 g15028 g15028.t4 isoform g15028.t4 3587302 3588366
chr_4 g15028 g15028.t4 exon g15028.t4.exon1 3587302 3587768
chr_4 g15028 g15028.t4 cds g15028.t4.CDS1 3587302 3587768
chr_4 g15028 g15028.t4 exon g15028.t4.exon2 3587838 3588366
chr_4 g15028 g15028.t4 cds g15028.t4.CDS2 3587838 3588045
chr_4 g15028 g15028.t4 TSS g15028.t4 NA NA

Sequences

>g15028.t4 Gene=g15028 Length=996
TGGTGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGT
TTTAGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAA
AATTTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGAT
AATTTAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTA
AATTTCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTA
GTTGTCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAA
AATTCTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAG
CTTGCTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACC
CTTAATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTGGTGG
AACGCAAATAACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTGGTGT
GTGAGACATTTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTCTGGA
GTTGTTTTTTTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCGAACA
TTTAAAATTTGGGCTTTTATGGGAATGGTAATAATTAATCTAAATTATAAAGTTGTATTC
ATTTTAAAAATTTATTATATAAATTCACAGGTTTCGCAGTTACCTCTTTCTTATGTATCA
AAATTTCTCGAAAAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTTA
ATTTTAGGGCAACCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTAT
AATAAAGTTATACTTGAACCTGAATATTTAGAATAG

>g15028.t4 Gene=g15028 Length=224
MALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLLGEI
LHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSRFAASGVVFFISA
FFHEYLVSVPLRTFKIWAFMGMVIINLNYKVVFILKIYYINSQVSQLPLSYVSKFLEKNV
GERWGNILVWGSLILGQPLCIMVYYHDYVIQNYNKVILEPEYLE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g15028.t4 PANTHER PTHR10408:SF7 DIACYLGLYCEROL O-ACYLTRANSFERASE 1 2 212 8.8E-80
3 g15028.t4 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 2 212 8.8E-80
1 g15028.t4 Pfam PF03062 MBOAT, membrane-bound O-acyltransferase family 2 142 2.7E-21
9 g15028.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 30 -
16 g15028.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 31 50 -
11 g15028.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 51 108 -
14 g15028.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 109 127 -
10 g15028.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 128 138 -
15 g15028.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 139 160 -
12 g15028.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 161 186 -
13 g15028.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 187 205 -
8 g15028.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 206 224 -
5 g15028.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 40 62 -
7 g15028.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 109 131 -
6 g15028.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 138 160 -
4 g15028.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 184 206 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004144 diacylglycerol O-acyltransferase activity MF
GO:0019432 triglyceride biosynthetic process BP
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values