Gene loci information

Transcript annotation

  • This transcript has been annotated as Diacylglycerol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15028 g15028.t5 TTS g15028.t5 3587251 3587251
chr_4 g15028 g15028.t5 isoform g15028.t5 3587302 3588612
chr_4 g15028 g15028.t5 exon g15028.t5.exon1 3587302 3587487
chr_4 g15028 g15028.t5 cds g15028.t5.CDS1 3587302 3587487
chr_4 g15028 g15028.t5 exon g15028.t5.exon2 3587551 3587768
chr_4 g15028 g15028.t5 cds g15028.t5.CDS2 3587551 3587768
chr_4 g15028 g15028.t5 exon g15028.t5.exon3 3587838 3588375
chr_4 g15028 g15028.t5 cds g15028.t5.CDS3 3587838 3588375
chr_4 g15028 g15028.t5 exon g15028.t5.exon4 3588438 3588612
chr_4 g15028 g15028.t5 cds g15028.t5.CDS4 3588438 3588503
chr_4 g15028 g15028.t5 TSS g15028.t5 NA NA

Sequences

>g15028.t5 Gene=g15028 Length=1117
GGAGATTATTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGT
TCTTTTGCCAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAAC
ATTTGTCTGTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAA
TTTATGGTGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGA
AAGTTTTAGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAA
TGAAAATTTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCC
TGATAATTTAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGA
ATTAAATTTCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGA
AGTAGTTGTCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGT
TAAAAATTCTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATT
GAAGCTTGCTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTC
AACCCTTAATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTG
GTGGAACGCAAATAACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTG
GTGTGTGAGACATTTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTC
TGGAGTTGTTTTTTTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCG
AACATTTAAAATTTGGGCTTTTATGGGAATGGTTTCGCAGTTACCTCTTTCTTATGTATC
AAAATTTCTCGAAAAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTT
AATTTTAGGGCAACCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTA
TAATAAAGTTATACTTGAACCTGAATATTTAGAATAG

>g15028.t5 Gene=g15028 Length=335
MGSTFVCFLYCILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSN
TDQNENFIANDTKDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIK
RILEVVVGVNIVMALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYL
IFHSTLNLLGEILHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSR
FAASGVVFFISAFFHEYLVSVPLRTFKIWAFMGMVSQLPLSYVSKFLEKNVGERWGNILV
WGSLILGQPLCIMVYYHDYVIQNYNKVILEPEYLE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g15028.t5 PANTHER PTHR10408:SF7 DIACYLGLYCEROL O-ACYLTRANSFERASE 1 2 323 6.8E-122
3 g15028.t5 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 2 323 6.8E-122
27 g15028.t5 PIRSF PIRSF000439 Oat_ACAT_DAG_ARE 1 326 3.6E-116
1 g15028.t5 Pfam PF03062 MBOAT, membrane-bound O-acyltransferase family 5 315 2.5E-31
17 g15028.t5 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
18 g15028.t5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 3 -
19 g15028.t5 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 4 15 -
26 g15028.t5 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 16 19 -
13 g15028.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 91 -
24 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 92 110 -
12 g15028.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 111 121 -
22 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 122 141 -
16 g15028.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 142 171 -
21 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 172 190 -
10 g15028.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 191 236 -
25 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 237 254 -
15 g15028.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 255 265 -
23 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 266 283 -
11 g15028.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 284 294 -
20 g15028.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 295 316 -
14 g15028.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 317 335 -
9 g15028.t5 SignalP_EUK SignalP-noTM SignalP-noTM 1 23 -
5 g15028.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 4 26 -
6 g15028.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 122 141 -
4 g15028.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 171 193 -
8 g15028.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 237 259 -
7 g15028.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 295 317 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004144 diacylglycerol O-acyltransferase activity MF
GO:0019432 triglyceride biosynthetic process BP
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values