General information for g15086.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d10.
Parent gene
- The parent gene g15086 is located on chr_4: 3855817..3856861 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15086 | g15086.t1 | TSS | g15086.t1 | 3854791 | 3854791 |
| chr_4 | g15086 | g15086.t1 | isoform | g15086.t1 | 3854821 | 3856861 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon1 | 3854821 | 3854930 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS1 | 3854821 | 3854930 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon2 | 3855041 | 3855255 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS2 | 3855041 | 3855255 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon3 | 3855357 | 3855572 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS3 | 3855357 | 3855572 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon4 | 3855663 | 3855732 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS4 | 3855663 | 3855732 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon5 | 3855786 | 3856075 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS5 | 3855786 | 3856075 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon6 | 3856133 | 3856372 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS6 | 3856133 | 3856372 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon7 | 3856475 | 3856633 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS7 | 3856475 | 3856633 |
| chr_4 | g15086 | g15086.t1 | exon | g15086.t1.exon8 | 3856686 | 3856861 |
| chr_4 | g15086 | g15086.t1 | cds | g15086.t1.CDS8 | 3856686 | 3856861 |
| chr_4 | g15086 | g15086.t1 | TTS | g15086.t1 | 3856885 | 3856885 |
Sequences
>g15086.t1 Gene=g15086 Length=1476
ATGGAAACGTTTACTTTTGTACTTATTTTATTTTTTCTATTACTTGCTTGGTATTATAGT
GAATATCGATTTCGTTTCCAATCGATTTCTAAATTTCCGGGACCAAAAAGAATTCCGTTT
TTTGGCAATGCGTTAATGTTCATTGGTAAAAAGCCGTCAGAAATTTTGACAATTGTTGAG
CAATTACATCGTGAATATGGAAATGTTTTGTGCCTTATGATGGGTTCACAACCTGAAATA
TTATTAACAAGTCCCGAAGACGTTGAAGTTGTTTTGGGAAGTTCAAAATTGATTAAAAAA
TCTGATGAATATGATTTTATGACAGATTGGCTCGGCACTGGATTATTAATTGCAAGTGGA
CTAAAATGGTTTCAAAAAAGAAAAATAACCACGCCAGCTTTTCATCTTCAAATTCTTGAT
CAATTTGTTGAAATTTTTGACAAACATAGTGCAATTTTTGTAAAAAATCTCGCAAAGTCT
GAAGGTCATTCAATAGATGTTTTTCAGCCAATTACACTTTGCGCTCTAGACATTATCTGC
GAGACTGCTATGGGAATTGAAATTCACGCTCAGGCAAATTCAAATTCTGATTATGTTCGT
GCTGTTAAAGAAATGAGTAATTTAGCAATGGCAAGAAGCGTCAATTTTTTCTTAAGAAAT
AAAATTATTTATAAATTTTCATCAATGAAAAGACGACAAGATAAATTGTTAAAAATTTTA
CATGGTTTTACTGATGATGTTATAATTTCAAGACGTGAAGAATTGATTAAAAATCAAGAA
ATTTCGCAAAATAAGAAAAAGAAGATGGCCTTTCTTGATGTTCTTTTACAATCGACAATC
GAAGGAAAACCATTGTCAAATATGGACATAAGAGAAGAAGTTGATATTTTTATGTTTGAA
GGGTTTGACACAACTAGCAGTGGAATTGCTTTTTGCCTTTACAATCTTGCTAAATATCCT
GCAGTGCAGAAAAAAGTTTTTGAAGAAATAATTAAAGTTATTGGTGCTGATAAAGACAAA
TCAGTGACATTGACAAATTTAAATGATTTAAAATATCTTGAAATGGTGATTAAAGAAACT
TTAAGACTTTATCCTAGTGTTCCAGCTTATGGCAGAAAAATGGTTGAAAATGTTGAAATT
AATGGAAAATTAATACCACGTGGAACTAATATTGGAATAGGACCCTATTTTATGGCACGC
GATGCTTCTTTATGGAAAAATCCTTTAGAATTCATTCCTGAAAGATTTAGCACAAATGAA
TCTCAATATCATCCTTTTCTTCATGTTCCGTTTTCTGCTGGTCCTAGAAATTGTGTAGGC
CAAAAATTTGCAATGCTTGAAATGAAAAGTGTTGTTTCAAAAATTCTTAGAAATTATGAA
ATTCAAGTTGATAGTGATTTTAAATTAGTTTTACTTTTTGAAGTTATTTTAAGACCAGAA
AATGGAGTTTTTTTGAAAATGATTAAAAGAAAATGA
>g15086.t1 Gene=g15086 Length=491
METFTFVLILFFLLLAWYYSEYRFRFQSISKFPGPKRIPFFGNALMFIGKKPSEILTIVE
QLHREYGNVLCLMMGSQPEILLTSPEDVEVVLGSSKLIKKSDEYDFMTDWLGTGLLIASG
LKWFQKRKITTPAFHLQILDQFVEIFDKHSAIFVKNLAKSEGHSIDVFQPITLCALDIIC
ETAMGIEIHAQANSNSDYVRAVKEMSNLAMARSVNFFLRNKIIYKFSSMKRRQDKLLKIL
HGFTDDVIISRREELIKNQEISQNKKKKMAFLDVLLQSTIEGKPLSNMDIREEVDIFMFE
GFDTTSSGIAFCLYNLAKYPAVQKKVFEEIIKVIGADKDKSVTLTNLNDLKYLEMVIKET
LRLYPSVPAYGRKMVENVEINGKLIPRGTNIGIGPYFMARDASLWKNPLEFIPERFSTNE
SQYHPFLHVPFSAGPRNCVGQKFAMLEMKSVVSKILRNYEIQVDSDFKLVLLFEVILRPE
NGVFLKMIKRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g15086.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 15 | 491 | 2.2E-125 |
| 2 | g15086.t1 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 7 | 490 | 8.5E-170 |
| 3 | g15086.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 7 | 490 | 8.5E-170 |
| 5 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 63 | 82 | 2.3E-27 |
| 4 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 289 | 306 | 2.3E-27 |
| 12 | g15086.t1 | PRINTS | PR00385 | P450 superfamily signature | 300 | 317 | 9.2E-14 |
| 7 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 309 | 335 | 2.3E-27 |
| 10 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 354 | 372 | 2.3E-27 |
| 11 | g15086.t1 | PRINTS | PR00385 | P450 superfamily signature | 355 | 366 | 9.2E-14 |
| 9 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 394 | 418 | 2.3E-27 |
| 6 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 428 | 438 | 2.3E-27 |
| 14 | g15086.t1 | PRINTS | PR00385 | P450 superfamily signature | 429 | 438 | 9.2E-14 |
| 8 | g15086.t1 | PRINTS | PR00463 | E-class P450 group I signature | 438 | 461 | 2.3E-27 |
| 13 | g15086.t1 | PRINTS | PR00385 | P450 superfamily signature | 438 | 449 | 9.2E-14 |
| 1 | g15086.t1 | Pfam | PF00067 | Cytochrome P450 | 33 | 486 | 1.0E-117 |
| 18 | g15086.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 19 | g15086.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 20 | g15086.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 21 | g15086.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 17 | g15086.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 491 | - |
| 23 | g15086.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 431 | 440 | - |
| 15 | g15086.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 22 | 490 | 6.55E-120 |
| 22 | g15086.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K17952 (cytochrome P450 family 29 subfamily A)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed