General information for g15086.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d10.
Parent gene
- The parent gene g15086 is located on chr_4: 3855817..3856861 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15086 | g15086.t10 | TSS | g15086.t10 | 3854791 | 3854791 |
| chr_4 | g15086 | g15086.t10 | isoform | g15086.t10 | 3854821 | 3856861 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon1 | 3854821 | 3854930 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS1 | 3854821 | 3854930 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon2 | 3855041 | 3855255 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS2 | 3855041 | 3855255 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon3 | 3855357 | 3855572 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS3 | 3855357 | 3855572 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon4 | 3855663 | 3855732 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS4 | 3855663 | 3855732 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon5 | 3855786 | 3856075 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS5 | 3855786 | 3856075 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon6 | 3856149 | 3856372 |
| chr_4 | g15086 | g15086.t10 | cds | g15086.t10.CDS6 | 3856149 | 3856222 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon7 | 3856475 | 3856633 |
| chr_4 | g15086 | g15086.t10 | exon | g15086.t10.exon8 | 3856704 | 3856861 |
| chr_4 | g15086 | g15086.t10 | TTS | g15086.t10 | 3856885 | 3856885 |
Sequences
>g15086.t10 Gene=g15086 Length=1442
ATGGAAACGTTTACTTTTGTACTTATTTTATTTTTTCTATTACTTGCTTGGTATTATAGT
GAATATCGATTTCGTTTCCAATCGATTTCTAAATTTCCGGGACCAAAAAGAATTCCGTTT
TTTGGCAATGCGTTAATGTTCATTGGTAAAAAGCCGTCAGAAATTTTGACAATTGTTGAG
CAATTACATCGTGAATATGGAAATGTTTTGTGCCTTATGATGGGTTCACAACCTGAAATA
TTATTAACAAGTCCCGAAGACGTTGAAGTTGTTTTGGGAAGTTCAAAATTGATTAAAAAA
TCTGATGAATATGATTTTATGACAGATTGGCTCGGCACTGGATTATTAATTGCAAGTGGA
CTAAAATGGTTTCAAAAAAGAAAAATAACCACGCCAGCTTTTCATCTTCAAATTCTTGAT
CAATTTGTTGAAATTTTTGACAAACATAGTGCAATTTTTGTAAAAAATCTCGCAAAGTCT
GAAGGTCATTCAATAGATGTTTTTCAGCCAATTACACTTTGCGCTCTAGACATTATCTGC
GAGACTGCTATGGGAATTGAAATTCACGCTCAGGCAAATTCAAATTCTGATTATGTTCGT
GCTGTTAAAGAAATGAGTAATTTAGCAATGGCAAGAAGCGTCAATTTTTTCTTAAGAAAT
AAAATTATTTATAAATTTTCATCAATGAAAAGACGACAAGATAAATTGTTAAAAATTTTA
CATGGTTTTACTGATGATGTTATAATTTCAAGACGTGAAGAATTGATTAAAAATCAAGAA
ATTTCGCAAAATAAGAAAAAGAAGATGGCCTTTCTTGATGTTCTTTTACAATCGACAATC
GAAGGAAAACCATTGTCAAATATGGACATAAGAGAAGAAGTTGATATTTTTATGTTTGAA
GCAGTGGAATTGCTTTTTGCCTTTACAATCTTGCTAAATATCCTGCAGTGCAGAAAAAAG
TTTTTGAAGAAATAATTAAAGTTATTGGTGCTGATAAAGACAAATCAGTGACATTGACAA
ATTTAAATGATTTAAAATATCTTGAAATGGTGATTAAAGAAACTTTAAGACTTTATCCTA
GTGTTCCAGCTTATGGCAGAAAAATGGTTGAAAATGTTGAAATTAATGGAAAATTAATAC
CACGTGGAACTAATATTGGAATAGGACCCTATTTTATGGCACGCGATGCTTCTTTATGGA
AAAATCCTTTAGAATTCATTCCTGAAAGATTTAGCACAAATGAATCTCAATATCATCCTT
TTCTTCATGTTCCGTTTTCTGCTGGCCAAAAATTTGCAATGCTTGAAATGAAAAGTGTTG
TTTCAAAAATTCTTAGAAATTATGAAATTCAAGTTGATAGTGATTTTAAATTAGTTTTAC
TTTTTGAAGTTATTTTAAGACCAGAAAATGGAGTTTTTTTGAAAATGATTAAAAGAAAAT
GA
>g15086.t10 Gene=g15086 Length=324
METFTFVLILFFLLLAWYYSEYRFRFQSISKFPGPKRIPFFGNALMFIGKKPSEILTIVE
QLHREYGNVLCLMMGSQPEILLTSPEDVEVVLGSSKLIKKSDEYDFMTDWLGTGLLIASG
LKWFQKRKITTPAFHLQILDQFVEIFDKHSAIFVKNLAKSEGHSIDVFQPITLCALDIIC
ETAMGIEIHAQANSNSDYVRAVKEMSNLAMARSVNFFLRNKIIYKFSSMKRRQDKLLKIL
HGFTDDVIISRREELIKNQEISQNKKKKMAFLDVLLQSTIEGKPLSNMDIREEVDIFMFE
AVELLFAFTILLNILQCRKKFLKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g15086.t10 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 15 | 307 | 6.5E-49 |
| 2 | g15086.t10 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 7 | 301 | 2.2E-91 |
| 3 | g15086.t10 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 7 | 301 | 2.2E-91 |
| 1 | g15086.t10 | Pfam | PF00067 | Cytochrome P450 | 33 | 303 | 3.0E-48 |
| 10 | g15086.t10 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 11 | g15086.t10 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g15086.t10 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 14 | g15086.t10 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 9 | g15086.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 295 | - |
| 13 | g15086.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 296 | 315 | - |
| 8 | g15086.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 316 | 324 | - |
| 6 | g15086.t10 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 22 | 303 | 8.51E-49 |
| 5 | g15086.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 22 | - |
| 4 | g15086.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 296 | 315 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed