Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 2J3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15164 g15164.t2 isoform g15164.t2 4193928 4199964
chr_4 g15164 g15164.t2 exon g15164.t2.exon1 4193928 4194518
chr_4 g15164 g15164.t2 cds g15164.t2.CDS1 4194149 4194518
chr_4 g15164 g15164.t2 exon g15164.t2.exon2 4195296 4195328
chr_4 g15164 g15164.t2 cds g15164.t2.CDS2 4195296 4195328
chr_4 g15164 g15164.t2 exon g15164.t2.exon3 4199918 4199964
chr_4 g15164 g15164.t2 cds g15164.t2.CDS3 4199918 4199922
chr_4 g15164 g15164.t2 TTS g15164.t2 4200119 4200119
chr_4 g15164 g15164.t2 TSS g15164.t2 NA NA

Sequences

>g15164.t2 Gene=g15164 Length=671
AGTATGATAAATATTCTTTGGACTTTCATAACTGGATCAAAAATAAACGACCAACAATCC
AAATCTAATGAAAATTTTAGAAATATTAGAAAAACGAGGAAAAGCATTTGATCTCAGTGG
TGGATATTTAAATCAAATGCCATGGCTGAGATTCATTGCTCCAGAAGCAACTGGTTATAA
TTTAATTCAAAAATTCAATTCAGCATTTTATGATTTTTTTCATGGATATAATTAATGATC
ATATAAAATCATACAGCGATGACAAAACAGGTGATGATGTCATTTATGCTTACATCAAGG
AAATGAATGCACAAAAAGATAGAGAAGATACAACATTCAAACTTGAACAGCTTGTTTTCA
TCATTTTGGACATTTTTATAGCTGGTGCAAGTTCAACAGCAATTACAATTGATTTAGCAT
TGATGTCAATGTTGTTGTATCCAGAAATTCAAAGAAAAATTCATAAAGAAATTGATGAAG
TCCTTGGTGAAAATAGTGAAATGCCGAGATATATTGATAGAAATAGAATGCCATATACCG
AAGCTGTATTGCTTGAAGTTCAACGAATGTATAGTGTTGCTTCTGTATCAGGACCAAGAA
GAACAATGAAAGCAACAACTTTAGAATAGAGTCCTTACTACATGAAATTTTTTATATACG
TTCTCAATACA

>g15164.t2 Gene=g15164 Length=135
MDIINDHIKSYSDDKTGDDVIYAYIKEMNAQKDREDTTFKLEQLVFIILDIFIAGASSTA
ITIDLALMSMLLYPEIQRKIHKEIDEVLGENSEMPRYIDRNRMPYTEAVLLEVQRMYSVA
SVSGPRRTMKATTLE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g15164.t2 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 135 6.1E-36
2 g15164.t2 PANTHER PTHR24303:SF13 CYTOCHROME P450 305A1-RELATED 2 134 1.9E-38
3 g15164.t2 PANTHER PTHR24303 HEME-BINDING MONOOXYGENASE FAMILY 2 134 1.9E-38
5 g15164.t2 PRINTS PR00463 E-class P450 group I signature 43 60 1.0E-16
6 g15164.t2 PRINTS PR00463 E-class P450 group I signature 63 89 1.0E-16
4 g15164.t2 PRINTS PR00463 E-class P450 group I signature 107 125 1.0E-16
1 g15164.t2 Pfam PF00067 Cytochrome P450 3 134 4.4E-27
11 g15164.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 43 -
12 g15164.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 44 73 -
10 g15164.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 74 135 -
8 g15164.t2 SUPERFAMILY SSF48264 Cytochrome P450 2 134 6.68E-32
7 g15164.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 44 66 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed