General information for g15165.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 305a1.
Parent gene
- The parent gene g15165 is located on chr_4: 4206344..4209675 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15165 | g15165.t1 | TSS | g15165.t1 | 4206186 | 4206186 |
| chr_4 | g15165 | g15165.t1 | isoform | g15165.t1 | 4206344 | 4208728 |
| chr_4 | g15165 | g15165.t1 | exon | g15165.t1.exon1 | 4206344 | 4206443 |
| chr_4 | g15165 | g15165.t1 | cds | g15165.t1.CDS1 | 4206344 | 4206443 |
| chr_4 | g15165 | g15165.t1 | exon | g15165.t1.exon2 | 4207142 | 4207352 |
| chr_4 | g15165 | g15165.t1 | cds | g15165.t1.CDS2 | 4207142 | 4207352 |
| chr_4 | g15165 | g15165.t1 | exon | g15165.t1.exon3 | 4207470 | 4207708 |
| chr_4 | g15165 | g15165.t1 | cds | g15165.t1.CDS3 | 4207470 | 4207708 |
| chr_4 | g15165 | g15165.t1 | exon | g15165.t1.exon4 | 4208087 | 4208296 |
| chr_4 | g15165 | g15165.t1 | cds | g15165.t1.CDS4 | 4208087 | 4208296 |
| chr_4 | g15165 | g15165.t1 | exon | g15165.t1.exon5 | 4208352 | 4208728 |
| chr_4 | g15165 | g15165.t1 | cds | g15165.t1.CDS5 | 4208352 | 4208728 |
| chr_4 | g15165 | g15165.t1 | TTS | g15165.t1 | 4209680 | 4209680 |
Sequences
>g15165.t1 Gene=g15165 Length=1137
ATGTTGGAAATATTTCTTGCTTCTATTGCCTTAATTTTTGTCATAAGGGCAATTAGTGCA
ATTTTAAAAGAATTTAGACGACCTGAAAACTTCCCACCAGGTCCAAACTGGTTTTTACCA
TTTGTCGGCAGTTCAAGATACTTTAAAAAATTATGTCAACTATTTGGGGGACAACATAAA
GCTTTTGATTATTTGGCAAAGAAATACAACTCACCAATTTTTAGTATCAAACTTGGAAGA
GAGCTTGTGGTTGTCGTTACAACACATGAATTGATAAAAGAAGTTCATGCAAGAGAAGAA
TTTGATGGACGACCAGACAATTTCTTCATACGCCTTCGTACGATGGGAACACGTTTAGGT
ATCACTTCCAGTGATGGTCGCAATTGGCATGAACAAAGAAGTTTTGTTCTTCGTCAACTT
CGAAATGTTGGCTTTGGAAAAACAAAAATGGAAGAGCAAATACATGCAGAACTCAATGAA
ATTTTAGATATTATCAAAAGCAAGAAAGACATTCCAATTTGGCCAGGTGAAAGCAGTTTG
ATATCAACGACTGGTGCAAGTTCAACAGCAATTACAATTGATTTAGCATTGATGTCAATG
TTGTTATATCCAGAAATTCAAAAGAAAATTCATAAAGAAATTGATGAAGTCCTTGGTGAA
AATAGTGAAATGCCGAGATATATTGATAGGAATAGAATGCCATATACCGAAGCTGTATTG
CTTGAAGTTCAACGATTTTACAGTATTATTTCTGTGTCAGGACCAAGAAGAACAATGAAA
GCAACAACTTTAGGTGGTTACGATCTTCCAAAATATACAACAATTTTAATCGGTCTCGGA
ACAGTTCACAAAGATGAAGACTATTGGAAAGATCCACAAATTTTTCGACCAGAAAGATTC
TTGGATGAAAATAACAGCATTTGCAATACTGAACGACTTTTACCATTTGGTGCCGGTAGA
AGAAAGTGCTTGGGTGATCAACTTGCTAGAGCTGCACTTTTTACATTTTTTGTTGGAATT
TTGAAAGAGTTTTCCTTAGAAAAATGTGAAAATGCTTTGCCTAGCAAAGATTTAACACCA
GGATTGATGATGAGTCCAAAACCCTATAAAGCTTACTTTAGAAAGAGAAAAATTTAA
>g15165.t1 Gene=g15165 Length=378
MLEIFLASIALIFVIRAISAILKEFRRPENFPPGPNWFLPFVGSSRYFKKLCQLFGGQHK
AFDYLAKKYNSPIFSIKLGRELVVVVTTHELIKEVHAREEFDGRPDNFFIRLRTMGTRLG
ITSSDGRNWHEQRSFVLRQLRNVGFGKTKMEEQIHAELNEILDIIKSKKDIPIWPGESSL
ISTTGASSTAITIDLALMSMLLYPEIQKKIHKEIDEVLGENSEMPRYIDRNRMPYTEAVL
LEVQRFYSIISVSGPRRTMKATTLGGYDLPKYTTILIGLGTVHKDEDYWKDPQIFRPERF
LDENNSICNTERLLPFGAGRRKCLGDQLARAALFTFFVGILKEFSLEKCENALPSKDLTP
GLMMSPKPYKAYFRKRKI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g15165.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 22 | 180 | 2.9E-27 |
| 18 | g15165.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 181 | 378 | 2.4E-64 |
| 4 | g15165.t1 | PANTHER | PTHR24303:SF13 | CYTOCHROME P450 305A1-RELATED | 9 | 178 | 6.7E-133 |
| 6 | g15165.t1 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 9 | 178 | 6.7E-133 |
| 3 | g15165.t1 | PANTHER | PTHR24303:SF13 | CYTOCHROME P450 305A1-RELATED | 184 | 376 | 6.7E-133 |
| 5 | g15165.t1 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 184 | 376 | 6.7E-133 |
| 11 | g15165.t1 | PRINTS | PR00385 | P450 superfamily signature | 184 | 201 | 2.9E-9 |
| 12 | g15165.t1 | PRINTS | PR00385 | P450 superfamily signature | 238 | 249 | 2.9E-9 |
| 10 | g15165.t1 | PRINTS | PR00359 | B-class P450 signature | 256 | 283 | 5.2E-5 |
| 8 | g15165.t1 | PRINTS | PR00359 | B-class P450 signature | 284 | 299 | 5.2E-5 |
| 7 | g15165.t1 | PRINTS | PR00359 | B-class P450 signature | 314 | 323 | 5.2E-5 |
| 13 | g15165.t1 | PRINTS | PR00385 | P450 superfamily signature | 314 | 323 | 2.9E-9 |
| 9 | g15165.t1 | PRINTS | PR00359 | B-class P450 signature | 323 | 334 | 5.2E-5 |
| 14 | g15165.t1 | PRINTS | PR00385 | P450 superfamily signature | 323 | 334 | 2.9E-9 |
| 2 | g15165.t1 | Pfam | PF00067 | Cytochrome P450 | 32 | 172 | 2.0E-17 |
| 1 | g15165.t1 | Pfam | PF00067 | Cytochrome P450 | 185 | 371 | 1.6E-57 |
| 20 | g15165.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 21 | g15165.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 22 | g15165.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 23 | g15165.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 19 | g15165.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 378 | - |
| 16 | g15165.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 316 | 325 | - |
| 15 | g15165.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 32 | 376 | 2.47E-76 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K07419 (vitamin D 25-hydroxylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed