Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 305a1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15165 g15165.t7 TSS g15165.t7 4206186 4206186
chr_4 g15165 g15165.t7 isoform g15165.t7 4206344 4209675
chr_4 g15165 g15165.t7 exon g15165.t7.exon1 4206344 4206443
chr_4 g15165 g15165.t7 exon g15165.t7.exon2 4207142 4207352
chr_4 g15165 g15165.t7 exon g15165.t7.exon3 4207470 4208296
chr_4 g15165 g15165.t7 cds g15165.t7.CDS1 4208011 4208296
chr_4 g15165 g15165.t7 exon g15165.t7.exon4 4208352 4208867
chr_4 g15165 g15165.t7 cds g15165.t7.CDS2 4208352 4208728
chr_4 g15165 g15165.t7 exon g15165.t7.exon5 4208990 4209675
chr_4 g15165 g15165.t7 TTS g15165.t7 4209680 4209680

Sequences

>g15165.t7 Gene=g15165 Length=2340
ATGTTGGAAATATTTCTTGCTTCTATTGCCTTAATTTTTGTCATAAGGGCAATTAGTGCA
ATTTTAAAAGAATTTAGACGACCTGAAAACTTCCCACCAGGTCCAAACTGGTTTTTACCA
TTTGTCGGCAGTTCAAGATACTTTAAAAAATTATGTCAACTATTTGGGGGACAACATAAA
GCTTTTGATTATTTGGCAAAGAAATACAACTCACCAATTTTTAGTATCAAACTTGGAAGA
GAGCTTGTGGTTGTCGTTACAACACATGAATTGATAAAAGAAGTTCATGCAAGAGAAGAA
TTTGATGGACGACCAGACAATTTCTTCATACGCCTTCGTACGATGGGAACACGTTTAGGT
ATCACTTCCAGTGATGGTCGCAATTGGCATGAACAAAGAAGTTTTGTTCTTCGTCAACTT
CGAAATGTTGGCTTTGGAAAAACAAAAATGGAAGAGCAAATACATGCAGAACTCAATGAA
ATTTTAGATATTATCAAAAGCAAGAAAGACATTCCAATTTGGCCAGGTGAAAGCAGTTTG
ATATCAACGAGTATGATAAATATTCTTTGGACTTTCATAACTGGATCAAAAATCAACGCA
AAACAATCCAAATCTAATGAAAATTTTAGAAATATTAGAAAAACGAGGAAAAGCATTTGA
TCTCAGTGGTGGATATTTAAATCAAATGCCATGGCTGAGATTCATTGCTCAGAAGCAACT
GGTTATAATTTAATTCAAAATTCAATTCAGCATTTTATGATTTCTTCATGGATATAATTA
ATGATCATATAAAATCATACAGCGATGACAAAACAGGTGATGATGTCATTTATGCTTACA
TCAAGGAAATGAATGCACAAAAAGACAGAGAAAGATACAACATTCAAACTTGAACAGCTT
GTTTTCATCATTTTGGACATTTTTATAGCTGGTGCAAGTTCAACAGCAATTACAATTGAT
TTAGCATTGATGTCAATGTTGTTATATCCAGAAATTCAAAAGAAAATTCATAAAGAAATT
GATGAAGTCCTTGGTGAAAATAGTGAAATGCCGAGATATATTGATAGGAATAGAATGCCA
TATACCGAAGCTGTATTGCTTGAAGTTCAACGATTTTACAGTATTATTTCTGTGTCAGGA
CCAAGAAGAACAATGAAAGCAACAACTTTAGGTGGTTACGATCTTCCAAAATATACAACA
ATTTTAATCGGTCTCGGAACAGTTCACAAAGATGAAGACTATTGGAAAGATCCACAAATT
TTTCGACCAGAAAGATTCTTGGATGAAAATAACAGCATTTGCAATACTGAACGACTTTTA
CCATTTGGTGCCGGTAGAAGAAAGTGCTTGGGTGATCAACTTGCTAGAGCTGCACTTTTT
ACATTTTTTGTTGGAATTTTGAAAGAGTTTTCCTTAGAAAAATGTGAAAATGCTTTGCCT
AGCAAAGATTTAACACCAGGATTGATGATGAGTCCAAAACCCTATAAAGCTTACTTTAGA
AAGAGAAAAATTTAAACAATATTTTTTTTGAAGTCCCAATAGAAAATCTGACCGTTTTCC
TTTAAATTTTGAAATATTTTTTCAATATCAATCCATGTTTTTTTTTTGAAATATAAGAAA
CCATCACGAATAATGAAAATTCTATTTATTTCAAGATAACAATCATTAACAGTCAGTTGG
GCCAACAAATTGAATTTTTTAAATTGTTCAAAAAATATGTTTATAAAAAAATATTCGTAT
TCATTTTTATTTTTTTATTTTTGCCCTTAAGAGCTAATAGTATGAAGATTTTAAATTTAA
GCTAACAACCACTATGAAAATTTCTGGTTTTAAACGACAAATTAACGCTAATTTCATTTT
ATTTAAATTTTAATTCGCCATCAAATTATTTAAGAAGTGCGTTGACATATTCCTTAACTA
GTGGAAATACTTTCTTTGTATATAGAAGTCCATAAAACAATTTTTGTTAAGTATCATTTT
TAAGGCTTTTTTTCGTAAGTTTTGCAACTTTCTGATGTGAAAATTGGCGAATTTTTTAGG
CGCAGGTAATAATTGGTAAAATTGAACGAATAACAAATTTATATATAATAATTTCTTTAG
TTTTGTGGAATTTAGATTTTCAACTCAAAAGTGGATAAAGTGTTTGGATTGTCAAATTTT
TAATTGTTATATCATAATCTATAAATTGAACGGTTACTACGGACAATAACAGATATTTTA
ATTAATTGTACAATTTATACTTATCTTTTACTACTGCCTTCTTTTCTTGATTGTAAATTT
TTAAGAAAAATACTGTAAGGAATCAAAAAAATATAAAAGAAAGACATTTTTGTACTGAAA

>g15165.t7 Gene=g15165 Length=220
MHKKTEKDTTFKLEQLVFIILDIFIAGASSTAITIDLALMSMLLYPEIQKKIHKEIDEVL
GENSEMPRYIDRNRMPYTEAVLLEVQRFYSIISVSGPRRTMKATTLGGYDLPKYTTILIG
LGTVHKDEDYWKDPQIFRPERFLDENNSICNTERLLPFGAGRRKCLGDQLARAALFTFFV
GILKEFSLEKCENALPSKDLTPGLMMSPKPYKAYFRKRKI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g15165.t7 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 220 8.8E-72
2 g15165.t7 PANTHER PTHR24303:SF13 CYTOCHROME P450 305A1-RELATED 5 218 3.9E-81
3 g15165.t7 PANTHER PTHR24303 HEME-BINDING MONOOXYGENASE FAMILY 5 218 3.9E-81
6 g15165.t7 PRINTS PR00463 E-class P450 group I signature 15 32 1.2E-41
13 g15165.t7 PRINTS PR00385 P450 superfamily signature 26 43 6.9E-11
8 g15165.t7 PRINTS PR00463 E-class P450 group I signature 35 61 1.2E-41
7 g15165.t7 PRINTS PR00463 E-class P450 group I signature 79 97 1.2E-41
12 g15165.t7 PRINTS PR00385 P450 superfamily signature 80 91 6.9E-11
9 g15165.t7 PRINTS PR00463 E-class P450 group I signature 120 144 1.2E-41
5 g15165.t7 PRINTS PR00463 E-class P450 group I signature 155 165 1.2E-41
11 g15165.t7 PRINTS PR00385 P450 superfamily signature 156 165 6.9E-11
4 g15165.t7 PRINTS PR00463 E-class P450 group I signature 165 188 1.2E-41
10 g15165.t7 PRINTS PR00385 P450 superfamily signature 165 176 6.9E-11
1 g15165.t7 Pfam PF00067 Cytochrome P450 3 213 1.6E-63
17 g15165.t7 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 15 -
18 g15165.t7 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 16 45 -
16 g15165.t7 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 46 220 -
20 g15165.t7 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 158 167 -
14 g15165.t7 SUPERFAMILY SSF48264 Cytochrome P450 6 218 4.58E-67
19 g15165.t7 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 13 35 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed