General information for g15206.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as L-asparaginase.
Parent gene
- The parent gene g15206 is located on chr_4: 4471168..4472511 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15206 | g15206.t1 | isoform | g15206.t1 | 4463528 | 4472511 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon1 | 4463528 | 4463684 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS1 | 4463528 | 4463684 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon2 | 4464410 | 4464451 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS2 | 4464410 | 4464451 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon3 | 4464525 | 4464564 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS3 | 4464525 | 4464564 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon4 | 4464913 | 4465012 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS4 | 4464913 | 4465012 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon5 | 4465072 | 4465693 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS5 | 4465072 | 4465693 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon6 | 4465749 | 4465913 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS6 | 4465749 | 4465913 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon7 | 4465974 | 4466072 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS7 | 4465974 | 4466072 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon8 | 4468534 | 4468615 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS8 | 4468534 | 4468615 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon9 | 4468671 | 4468812 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS9 | 4468671 | 4468812 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon10 | 4468871 | 4468933 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS10 | 4468871 | 4468933 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon11 | 4471706 | 4471880 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS11 | 4471706 | 4471880 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon12 | 4471945 | 4472026 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS12 | 4471945 | 4472026 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon13 | 4472082 | 4472230 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS13 | 4472082 | 4472230 |
| chr_4 | g15206 | g15206.t1 | exon | g15206.t1.exon14 | 4472282 | 4472511 |
| chr_4 | g15206 | g15206.t1 | cds | g15206.t1.CDS14 | 4472282 | 4472511 |
| chr_4 | g15206 | g15206.t1 | TSS | g15206.t1 | NA | NA |
| chr_4 | g15206 | g15206.t1 | TTS | g15206.t1 | NA | NA |
Sequences
>g15206.t1 Gene=g15206 Length=2148
ATGTATTTTTCGAGTTCATTATTTCTTATTTCTGTCATCACTTTGATATCATTTGTAAAT
CTTAGCTTTCAACAATTTAGAAATGAGCCGGAACAAATTTGTAGAAGAATTTCTCAAAAT
GTTCATACAGTTACTCAAGTTTTAGTTCTTTATACTGGTGGCACAATTGGCATGGTTCCA
TATAATCATACTTATACAGAATTTGTTAATAAGAAAGGTGCATTTGCTGAAATAATTAAA
AATACACCGGAATTAAATGACAGAAAAAATGGAAAAAGACTTCTCAAAAATATGGAAACA
AACAGAAGAGTTGAATCTAAAAATTTTGTTCTACCTGAAACTCCATTTGTACCACAAAGA
ATTGGTTATACTTTAGTAGAATATGACAAACTTATTGATTCAACAAATATTGATGTTGAT
AATTGGATAAAAATTGCCACAGATATTCAAAATAATTACAATGATTATGATGCTTTTGTG
ATTCTTCATGGCACTGACACTCTTGCTTACACTGCTTCAATGCTCTCTTTTATGTTTGAA
AATCTCAGCAAAACTGTTGTTATTACTGGAAGTCAAGTTCCAATGAGTCAAAAAAATAGT
GATGGACTTGCGAATTTCCTTGGTGCTTTAGTTGTTGCTGGAAGTTTAAAAATTCCACAA
GTTGTAGTTTTTTTTGACAATAGAATTTTTCAAGGCAATCGAGTCACAAAAATTCACAGT
TTGAATTTCAATGCTTTTGCAAGTCCTAATTATCCTGTTTTATTTGATTTTAATAAATTA
GGACTTGATGTAAGAGCTTTCCCAACATGTGTAAAAACAAATCGTAATGAAAATGAATTG
ATAGTGTCAATGAAAATTACAAATAATGTTGCAATGATTAAACTTTATCCAACAATTCGA
ATTAATGTTGTGGAAGAATTTTTAAAAGCTCCCATTCAAGGTGCTGTCATTGAGAGTTTT
GGTGCTGGAAATATGCCAAAACTTAGAACGGGATTAATGAAAGCTATTGCAAGTGCTAGT
GATAGAGGATTGATTATCATCAATGTGTCATCATGTCTTAAAGGTGGAGTTGAACAACTT
TATGAAACTGGCGATTTGAAAGCTTATGGAGTTATTAGTGGTCAAGATATGACACCAATT
GCTGCTTTTACAAAATTAATGTATGTCACAAGTCTTAATATAGATTTAAAATCAAAGAAA
AAAGCAATGAAAGAAAGTCTTCGTGGTCCTTATTTTGGGAATTTGAATGAATGGCCAACT
TTGCCGATTGCTTCAGCGTATCTTCACTTAATAAAACTCAATGCTAAATTTGTCGACATA
ATTCATACTGATAGTTTTAAATATGGTGAAGATTATTCGGCTGGTTATATAGATTTAAAA
TCTAAGATTTTTTGCCCAATACTGGAGCTTTTCAACCAAACTGCAGCATTTATTGCAGTT
CAACTGATTATAATTGCCTTCATTGCAGTCACAATAGAGCAGTGCAATATTACACTGAAT
CAATTCAATCAGCAAGCAGCTTTAATAGTCTCAATTTTAATTCAAATGCAAACTGCTGGT
TTTAATTTGACAACTTTTCATTTTATTGGTCATAGTTTAGGAGCACAAATTTTTGGGCGT
GTTGGTTATCAATTGATTCAAAATTACCACTTTACTCCAACTAGAATCACAGGACTTGAT
CCAGCTGGTCCTTATTTTGGAAATTTGAATGAATGGCCAACTTTGCCGATTGCTTCAGCG
TATCCTTCACTTAATAAACTCAATGCTAAATTTGTCGACATAATTCATACTGATAGTTTT
AAATATGGTGAAGATTATTCGGCTGGTTATATAGATTTAAAATCTAAGATTTTTGGCCCA
ATACTGGAGCTTTTCAACCAAACTGCAGCATTTATTGCAGTTCAACTGATTGTAAAATAT
AATTGCCTTCATTGCAGTCACAATAAAGCAGTGCAATATTACACTGAATCAATTCAATCA
GGTTCTGTAAGAAAATTCATATCAAATTTATGTTCTGGTCTTGCATATAACACTGGATCT
TGCTATGGCTCTGTTGCATCGATGGGCTTTTATGCTGATGAATATTCTGCTAATCCTGGC
AATTATTCTCTTAAAACTTACAGTGTTTCACCATATTCTACTTCTTAA
>g15206.t1 Gene=g15206 Length=715
MYFSSSLFLISVITLISFVNLSFQQFRNEPEQICRRISQNVHTVTQVLVLYTGGTIGMVP
YNHTYTEFVNKKGAFAEIIKNTPELNDRKNGKRLLKNMETNRRVESKNFVLPETPFVPQR
IGYTLVEYDKLIDSTNIDVDNWIKIATDIQNNYNDYDAFVILHGTDTLAYTASMLSFMFE
NLSKTVVITGSQVPMSQKNSDGLANFLGALVVAGSLKIPQVVVFFDNRIFQGNRVTKIHS
LNFNAFASPNYPVLFDFNKLGLDVRAFPTCVKTNRNENELIVSMKITNNVAMIKLYPTIR
INVVEEFLKAPIQGAVIESFGAGNMPKLRTGLMKAIASASDRGLIIINVSSCLKGGVEQL
YETGDLKAYGVISGQDMTPIAAFTKLMYVTSLNIDLKSKKKAMKESLRGPYFGNLNEWPT
LPIASAYLHLIKLNAKFVDIIHTDSFKYGEDYSAGYIDLKSKIFCPILELFNQTAAFIAV
QLIIIAFIAVTIEQCNITLNQFNQQAALIVSILIQMQTAGFNLTTFHFIGHSLGAQIFGR
VGYQLIQNYHFTPTRITGLDPAGPYFGNLNEWPTLPIASAYPSLNKLNAKFVDIIHTDSF
KYGEDYSAGYIDLKSKIFGPILELFNQTAAFIAVQLIVKYNCLHCSHNKAVQYYTESIQS
GSVRKFISNLCSGLAYNTGSCYGSVASMGFYADEYSANPGNYSLKTYSVSPYSTS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 23 | g15206.t1 | CDD | cd08963 | L-asparaginase_I | 46 | 389 | 6.26907E-99 |
| 13 | g15206.t1 | Gene3D | G3DSA:3.40.50.1170 | - | 44 | 286 | 9.5E-76 |
| 11 | g15206.t1 | Gene3D | G3DSA:3.40.50.40 | - | 288 | 411 | 5.5E-36 |
| 12 | g15206.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 483 | 715 | 5.2E-35 |
| 4 | g15206.t1 | PANTHER | PTHR11707 | L-ASPARAGINASE | 39 | 409 | 1.6E-118 |
| 22 | g15206.t1 | PIRSF | PIRSF001220 | L-ASNase_gatD | 42 | 99 | 0.002 |
| 19 | g15206.t1 | PIRSF | PIRSF500176 | L_ASNase | 43 | 101 | 2.9E-4 |
| 21 | g15206.t1 | PIRSF | PIRSF001220 | L-ASNase_gatD | 96 | 409 | 1.6E-72 |
| 20 | g15206.t1 | PIRSF | PIRSF500176 | L_ASNase | 104 | 409 | 7.4E-84 |
| 7 | g15206.t1 | PRINTS | PR00139 | Asparaginase/glutaminase family signature | 47 | 58 | 9.1E-14 |
| 5 | g15206.t1 | PRINTS | PR00139 | Asparaginase/glutaminase family signature | 157 | 175 | 9.1E-14 |
| 6 | g15206.t1 | PRINTS | PR00139 | Asparaginase/glutaminase family signature | 339 | 357 | 9.1E-14 |
| 1 | g15206.t1 | Pfam | PF00710 | Asparaginase, N-terminal | 46 | 259 | 4.0E-59 |
| 3 | g15206.t1 | Pfam | PF17763 | Glutaminase/Asparaginase C-terminal domain | 289 | 402 | 7.2E-22 |
| 2 | g15206.t1 | Pfam | PF00151 | Lipase | 480 | 712 | 2.2E-20 |
| 15 | g15206.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 18 | g15206.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 23 | - |
| 14 | g15206.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 24 | 469 | - |
| 17 | g15206.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 492 | - |
| 16 | g15206.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 493 | 715 | - |
| 27 | g15206.t1 | ProSitePatterns | PS00917 | Asparaginase / glutaminase active site signature 2. | 158 | 168 | - |
| 28 | g15206.t1 | ProSiteProfiles | PS51732 | Asparaginase / glutaminase domain profile. | 45 | 406 | 54.17 |
| 29 | g15206.t1 | SFLD | SFLDS00057 | Glutaminase/Asparaginase | 44 | 409 | 0.0 |
| 26 | g15206.t1 | SMART | SM00870 | Asparaginase_2 | 46 | 400 | 7.4E-98 |
| 8 | g15206.t1 | SUPERFAMILY | SSF53774 | Glutaminase/Asparaginase | 39 | 410 | 7.33E-89 |
| 9 | g15206.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 500 | 712 | 1.38E-19 |
| 10 | g15206.t1 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 24 | - |
| 25 | g15206.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 492 | - |
| 24 | g15206.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 508 | 530 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004806 | triglyceride lipase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01424 (L-asparaginase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed