General information for g15288.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 6a20.
Parent gene
- The parent gene g15288 is located on chr_4: 4838149..4839677 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15288 | g15288.t2 | isoform | g15288.t2 | 4837691 | 4839045 |
| chr_4 | g15288 | g15288.t2 | exon | g15288.t2.exon1 | 4837691 | 4838908 |
| chr_4 | g15288 | g15288.t2 | cds | g15288.t2.CDS1 | 4838253 | 4838908 |
| chr_4 | g15288 | g15288.t2 | exon | g15288.t2.exon2 | 4839027 | 4839045 |
| chr_4 | g15288 | g15288.t2 | cds | g15288.t2.CDS2 | 4839027 | 4839045 |
| chr_4 | g15288 | g15288.t2 | TTS | g15288.t2 | 4839920 | 4839920 |
| chr_4 | g15288 | g15288.t2 | TSS | g15288.t2 | NA | NA |
Sequences
>g15288.t2 Gene=g15288 Length=1237
ATGAAATTGAAAACACAAACCTTTGAAATTCGCAGCAGTCCAGGTATTTATATCACCAGA
AGATTATTTTAATGAAAAAATGTGTACAATTTTGTTAAAAAATTTATTCAAAATTTGATT
AAATTTAATTAAAAAATATTTAAATAAATAAATTAATCGATATGACCTTGAGAAAGTAAA
TGTGTAGGTTGTCATATATTTACACAATTAACTCAAATTCAGATTCTCTCTCGAGCACGC
ATACAAATGCTATAAAATTAATGTTTTGATGTTTATATTCACTTGATAGAATTTTTCTTC
TCTCAGTTTATAAAATTCATTAACACGGACAATATCGCAAAAAATGTTTTTCTATATAAT
TGCATTTTTAATTTTCTTAATTTATTTATATTTTAAATGATTATATTCTTATTGGGATCT
CCGCGGTTTTCCGTACGTTCCTGGAAAGTTTCCTTTAGGAAGTGTTGGAACAGTAGGATT
TAAAGAGCATTCTTGTGAATTCTTTAAGCGACAATATGACAAATTTAAAGATAAAGCTCC
TGCTTTTGGTGTATTTTTTTTTATGCAGACATCACTTGCAATTACTGACCCTGAATTGAT
TAAAGAAGTTTTTGTAAGAAATTTTGAAAGTTTTCATGAGCGTGGATTTTTTATTAATGA
ACAAGCTGATCCATTATCAGCTCATTTGTTTGCTCTTGGTGGTCAACAATGGAAAGAGTT
GAGGGCAAAATTGAGTCCCACATTTACATCAGGACGAATGAAAATGATGTTTCCATTGGT
AGTTAAAGCAGCTGATCGAATGGTTGAATATTTAATGCCATATGCAGACAACATTGAAGA
ATTAGAAGTTAAAGAAGTTTATGCAGAATTCACTACTGAAGTTATTTCTAGTGTTGCTTT
TGGATTAGACATTCCTTGTCTAGGAAATCCAGATAGTGAATTTAGAAAAGTTTCAAAAAC
ACTCTTTGAACCGACAAAATTAGAAAATATAAAAATAATGTTCATTTTTGCTTTTCCTGA
ACTTGCAAAAGGGTTAAATATGAGGTTTATTCCAAAGAAATCAGCAGATTTTTTCATGGG
AGTTATTCGAGATACGCTAGAATATCGTGAAAAAAATAATGTTGAGAGAAATGACTTTTT
GCAACTGTTGATAAACATAAGAGATACAGAAGGAATGACTTTCAATGAAATTGCTGCAAA
CTCTTTTATTTTCTTTAATGCTGGTTTCGAGACAAGC
>g15288.t2 Gene=g15288 Length=225
MQTSLAITDPELIKEVFVRNFESFHERGFFINEQADPLSAHLFALGGQQWKELRAKLSPT
FTSGRMKMMFPLVVKAADRMVEYLMPYADNIEELEVKEVYAEFTTEVISSVAFGLDIPCL
GNPDSEFRKVSKTLFEPTKLENIKIMFIFAFPELAKGLNMRFIPKKSADFFMGVIRDTLE
YREKNNVERNDFLQLLINIRDTEGMTFNEIAANSFIFFNAGFETS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g15288.t2 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 225 | 0 |
| 2 | g15288.t2 | PANTHER | PTHR24292 | CYTOCHROME P450 | 5 | 225 | 0 |
| 3 | g15288.t2 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 5 | 225 | 0 |
| 5 | g15288.t2 | PRINTS | PR00464 | Group II E-class P450 signature | 47 | 67 | 0 |
| 4 | g15288.t2 | PRINTS | PR00464 | Group II E-class P450 signature | 102 | 120 | 0 |
| 1 | g15288.t2 | Pfam | PF00067 | Cytochrome P450 | 2 | 225 | 0 |
| 6 | g15288.t2 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 4 | 225 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
