General information for g15289.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Cytochrome P450 6B1.
Parent gene
- The parent gene g15289 is located on chr_4: 4841320..4841929 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15289 | g15289.t4 | TSS | g15289.t4 | 4840314 | 4840314 |
| chr_4 | g15289 | g15289.t4 | isoform | g15289.t4 | 4841320 | 4841929 |
| chr_4 | g15289 | g15289.t4 | exon | g15289.t4.exon1 | 4841320 | 4841715 |
| chr_4 | g15289 | g15289.t4 | cds | g15289.t4.CDS1 | 4841423 | 4841715 |
| chr_4 | g15289 | g15289.t4 | exon | g15289.t4.exon2 | 4841773 | 4841929 |
| chr_4 | g15289 | g15289.t4 | cds | g15289.t4.CDS2 | 4841773 | 4841929 |
| chr_4 | g15289 | g15289.t4 | TTS | g15289.t4 | 4841976 | 4841976 |
Sequences
>g15289.t4 Gene=g15289 Length=553
TAGTTATGAATTGGCATTGAATCAAGACATACAAGATAAATTAAGAAATGAAATTAATGA
AGTTTTGGCCAATCACAATGGAGAAATCACTTATGATGCTATCATGGAAATGAAATATCT
TGACATGGTCTTTAATGAGTCATTGAGAAAATATCCAGGCGTTGATTCTCAAAATCGTAA
AGCAACAAAAGATTTTACTATACCTAATACTAACATAACAATTCCTGCTGGAACTTCTAT
AATGATTTCTTCATATGCGCTTCATAATGATGAAAGATTCTGGGAAAATCCTGAGAAATT
CGATCCAGACAGATTCTTGCCTGAAAATGTTCATAAACGTCATCCATTCTGTTACATTCC
ATTCAGTGAAGGCCCAAGACAGTGTATTGGATTGAGATTTGGTCAAATGCAGTCTAAAAT
TGGCATTGTAAATTTAATAAAGAACTTTAGAATTCTACCATGCAGCAAGACAATTATTCC
AATGAAATTTTCACCAACTGCAGCTTTTCAATCACCTGTAGGTGGAATGTGGCTTAAATT
TGAAAAACTTTAA
>g15289.t4 Gene=g15289 Length=149
MEMKYLDMVFNESLRKYPGVDSQNRKATKDFTIPNTNITIPAGTSIMISSYALHNDERFW
ENPEKFDPDRFLPENVHKRHPFCYIPFSEGPRQCIGLRFGQMQSKIGIVNLIKNFRILPC
SKTIIPMKFSPTAAFQSPVGGMWLKFEKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g15289.t4 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 149 | 2.6E-52 |
| 2 | g15289.t4 | PANTHER | PTHR24292 | CYTOCHROME P450 | 1 | 147 | 7.9E-56 |
| 4 | g15289.t4 | PRINTS | PR00463 | E-class P450 group I signature | 49 | 73 | 1.0E-12 |
| 3 | g15289.t4 | PRINTS | PR00463 | E-class P450 group I signature | 84 | 94 | 1.0E-12 |
| 5 | g15289.t4 | PRINTS | PR00463 | E-class P450 group I signature | 94 | 117 | 1.0E-12 |
| 1 | g15289.t4 | Pfam | PF00067 | Cytochrome P450 | 1 | 137 | 1.3E-48 |
| 7 | g15289.t4 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 87 | 96 | - |
| 6 | g15289.t4 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 2 | 147 | 1.17E-50 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
