Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a13.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15292 g15292.t1 TSS g15292.t1 4846208 4846208
chr_4 g15292 g15292.t1 isoform g15292.t1 4846238 4847824
chr_4 g15292 g15292.t1 exon g15292.t1.exon1 4846238 4847115
chr_4 g15292 g15292.t1 cds g15292.t1.CDS1 4846238 4847115
chr_4 g15292 g15292.t1 exon g15292.t1.exon2 4847179 4847607
chr_4 g15292 g15292.t1 cds g15292.t1.CDS2 4847179 4847607
chr_4 g15292 g15292.t1 exon g15292.t1.exon3 4847659 4847824
chr_4 g15292 g15292.t1 cds g15292.t1.CDS3 4847659 4847824
chr_4 g15292 g15292.t1 TTS g15292.t1 4847909 4847909

Sequences

>g15292.t1 Gene=g15292 Length=1473
ATGTTGATTTCAGTGCTCTTAATATTATTTTGCATTTATCTTTGGTTTTTAAATAAATTT
TCATATTGGAAAAAACTTGGCTTTCCATATTCACCTGGAAAATTTTTATTAGGCAGTGGA
CCAGGCATTGGCATTACAGAACATCCAAGTTATTTTGTAAAGCGTGAATATGAAAAATTT
AAAAATTCTGCACCAGCATTTGGAGCATATTTGATGGCATATCCAATCTTAGTGCCCACT
GATCCAGATTTAATCAGAGACATTTTTGTAAGAAAATATGACATTTTTCAAAATAGAGGA
TTTACAGTTAGTGAAAGAGCTGATCCATTATCGCAACATTTACTTTTTAAAAATGATGAT
GAATGGAAAAATTTAAGAACAAAACTGAGTCCAACATTTACCAGTTCTCGAATGAAAATG
ATGTTTACAAATGTAGCTGCCAAAGCTGATCGAATGGTTGAATATTTAATGCCATATGCA
GAAAAAAATGAATCTATTGAAATGAAAGAAATTTACAGTTCATTTTCTACTGAAATTATT
GCTGATGTTGCATTTGGGATCCAAATTAAATGCTTAGGAAATCCTGAAAATGAATTCAGA
AAAATGGCAAATATTGTCTTTCAACCGTCAATTTTGGAAAATTTAAAACTTTTCTTTTTG
ATTGCTTTTTCTAGAACTGCAACATTTCTTAACATTGGCTATACAAATCCAGAAATTATT
TCGTTCTTTATGAAGATTGTTAAAGAAACTTTGGATTATCGTCTTAAAAACAATCTTGAG
AGAAATGATTTCTTTCAATTATTAATGAATATTTACAAAACTGATGAGATTTCTTTTATT
GAATTTGCTGCTAATTCATATGTGTTTTTCTTAGCTGGTTTCGAGACAAGTTCAAGTGCA
ATGATGTTTAGTTCTTATGAGCTTGCATTAAATCAAGACATTCAAGGTAGACTTCGTACT
GAAATTAATGAAGTAATTGCAAAACACAATGGAAAAGTCACTTATGAGGCTATAATGGAA
ATGAAATATCTTGACATGGTGTTTAATGAGTCTATGAGAAAATTTCCAATTGTAGATTTA
CAGCATCGTAAGAATCTTAAAGATTATCGCATTCCTAACACAAATTTGGTAATTAAAGCT
GGAACTACAATAATGATTCCAGTTTACTGTCTACACAATGATGAAAGATTTTGGGAAAAT
CCAGATAAATTTGACCCTGAAAGATTCACATCTGAAAATATTAAGAAACGACATGCATTT
TGTTATATTCCATTTAGTGAAGGACCAAGACAGTGTATTGGCATGAGATTTGGTCAAATG
CAGACAAAAGTTGGACTTGTAAAGTTGTTGTTAAAATATAAAATTTTACCATGTGAAAAA
ACTATTTATCCTATGAAATTCGTTCCTAATGCAACATTTCAATGTCCTGTTGGTGGAATG
TGGTTGAAATTGGAAAAACTTGAAAATTCATGA

>g15292.t1 Gene=g15292 Length=490
MLISVLLILFCIYLWFLNKFSYWKKLGFPYSPGKFLLGSGPGIGITEHPSYFVKREYEKF
KNSAPAFGAYLMAYPILVPTDPDLIRDIFVRKYDIFQNRGFTVSERADPLSQHLLFKNDD
EWKNLRTKLSPTFTSSRMKMMFTNVAAKADRMVEYLMPYAEKNESIEMKEIYSSFSTEII
ADVAFGIQIKCLGNPENEFRKMANIVFQPSILENLKLFFLIAFSRTATFLNIGYTNPEII
SFFMKIVKETLDYRLKNNLERNDFFQLLMNIYKTDEISFIEFAANSYVFFLAGFETSSSA
MMFSSYELALNQDIQGRLRTEINEVIAKHNGKVTYEAIMEMKYLDMVFNESMRKFPIVDL
QHRKNLKDYRIPNTNLVIKAGTTIMIPVYCLHNDERFWENPDKFDPERFTSENIKKRHAF
CYIPFSEGPRQCIGMRFGQMQTKVGLVKLLLKYKILPCEKTIYPMKFVPNATFQCPVGGM
WLKLEKLENS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g15292.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 11 490 5.4E-125
2 g15292.t1 PANTHER PTHR24292 CYTOCHROME P450 5 487 7.3E-162
3 g15292.t1 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 5 487 7.3E-162
8 g15292.t1 PRINTS PR00463 E-class P450 group I signature 84 105 8.6E-18
7 g15292.t1 PRINTS PR00463 E-class P450 group I signature 281 298 8.6E-18
11 g15292.t1 PRINTS PR00385 P450 superfamily signature 292 309 3.5E-9
6 g15292.t1 PRINTS PR00463 E-class P450 group I signature 301 327 8.6E-18
10 g15292.t1 PRINTS PR00385 P450 superfamily signature 346 357 3.5E-9
5 g15292.t1 PRINTS PR00463 E-class P450 group I signature 387 411 8.6E-18
9 g15292.t1 PRINTS PR00463 E-class P450 group I signature 422 432 8.6E-18
13 g15292.t1 PRINTS PR00385 P450 superfamily signature 423 432 3.5E-9
4 g15292.t1 PRINTS PR00463 E-class P450 group I signature 432 455 8.6E-18
12 g15292.t1 PRINTS PR00385 P450 superfamily signature 432 443 3.5E-9
1 g15292.t1 Pfam PF00067 Cytochrome P450 36 468 6.6E-90
18 g15292.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
19 g15292.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
20 g15292.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 14 -
21 g15292.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 15 19 -
17 g15292.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 490 -
15 g15292.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 425 434 -
14 g15292.t1 SUPERFAMILY SSF48264 Cytochrome P450 20 485 1.18E-108

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values