General information for g15301.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neutral ceramidase.
Parent gene
- The parent gene g15301 is located on chr_4: 4893753..4895783 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15301 | g15301.t3 | isoform | g15301.t3 | 4893753 | 4895783 |
| chr_4 | g15301 | g15301.t3 | exon | g15301.t3.exon1 | 4893753 | 4894520 |
| chr_4 | g15301 | g15301.t3 | cds | g15301.t3.CDS1 | 4893803 | 4894520 |
| chr_4 | g15301 | g15301.t3 | exon | g15301.t3.exon2 | 4894603 | 4894796 |
| chr_4 | g15301 | g15301.t3 | cds | g15301.t3.CDS2 | 4894603 | 4894796 |
| chr_4 | g15301 | g15301.t3 | exon | g15301.t3.exon3 | 4894974 | 4895783 |
| chr_4 | g15301 | g15301.t3 | cds | g15301.t3.CDS3 | 4894974 | 4895783 |
| chr_4 | g15301 | g15301.t3 | TTS | g15301.t3 | 4895901 | 4895901 |
| chr_4 | g15301 | g15301.t3 | TSS | g15301.t3 | NA | NA |
Sequences
>g15301.t3 Gene=g15301 Length=1772
ATAGTGGACCAGCAGGATTTCATCAATTTGTCCTTTATGATCTCACAGCCATGGGTTTTG
TTGAGGAAACTTTCTATGCGATGGTTCGTGGTATAACACAGTCAATAATCAATGCTCATA
ATAACATGGTTGAAGGCAGAATTTTCCTATCTGAAACTGAAATTTTTGATGCAAACATCA
ATCGATCACCGCTGGCATATGAAAACAATCCAGATGAAGAAAAAGCACAATATAGAGATA
ATGTTGATAAAACTTTGTTTCAACTTCGATTTATGGATAAACAAAATAAAAAAATTCTTG
GTGCTTTTAATTGGTTTGCTGTCCATGCAACATCAATGAATAATACAAATAAATTTATCT
CATCTGATAATGTTGGTTATGCATCGATGCTGCTTGAAAAAGAATACAATTATGACAGCA
TGGTTGGAAAAGGCAGCTTCGTTGGTGCTTTTTGTTCTGCTAATTTAGGTGATGTCTCAC
CAAATATTATGGGACCAAAATGTCTTTCAACAGGATTACCATGCGATCCATTGACATCAA
AATGTCCAAATAAAGACGTTTGTGTTGCTTCTGGTCCAGGTAGAAATATTTATGAAAGTA
CAAGAATTATTGGTTCAAGAATTTACAAAGGTGCATCGAAATTGCTACAAGTGAAAGGTG
GAAGAGAAGTTACTGGACCATTGTCATTTGTTCATCAATTTATTGATATGCCAAAGCAAA
CTGGAGTTTATTTTAATCCTAAATTGAGAGGTTTACAGAATTACACTGGTTGTTATCCAG
CAATGGGTTATTCATTTGCAGCAGGTACAACTGATGGTCCAGGAGCTTTTGATTTTGCAC
AAGGAACTACAAGTGAAAATGCTTTTTGGAATGCTGTTCGTGATTTTATTGCTGAACCAA
GCATTCAAGACATTGCCTGTCATGGAAGTAAACCTATTTTAATAGCTACTGGACGTGCTG
TTTATCCATATCTCTGGCAGCCATCAGTTGTTCCTCTTCAAATTTTTGCCATCGGTGACC
TTTTAATGTTCGGCCTACCTGGTGAATTTACAACAATGGCTGGTCGTCGTTTAAAGAATG
AAATTCAGCTTTTTGCCAAAAGTCGATCTTATGAGTTTAACACAATTCTTTGTGGATTAT
CAAACATTTATACAAGTTATATAACAACACCAGAAGAATATGAAATTCAAAGATATGAAG
GTGCAAGTACAATCTTTGGTCCACATACATTGACAATTTATATAAATCGATTTATAAAAC
TACTTGATGCGATGTTAAGAGGAGCAGCTGTCGATCCAGGTCCAATGCCAATTGATCAAG
ATTCAAAGCAAATTTCGCTAGTGACAAAAGTTTATTATGATGGTCATGCAATTGGCAGTG
GTTTTGGATATGTCGTGACACAACCAAAGAAAAGTTATAGAAGAGGCCAAATTGTGACTT
GCAAATTTGTTGCTGGCAATCCAAGAAATAATTTTATGACTGATTCAAGTTATTTCTTTG
TTGAACGACTTGTTAGTAATGGTGAATGGCAGATGGTAGCAACTGATGCAGATTGGGAAA
CAAAATTCAAATGGACACGATTGTCAATGATTCTTGGTAGAAGTGAAATTGAATTTATGT
GGGAAATTCCAGAATCAACACCAAGTGGTGAATATCGTGTAAGACATCAAGGCTATTATC
AATACATTCTTGGTGGTGTCTATTATTATCAAGGATCAACTGAACATTTCAATATTTTTG
ATGATAACAATTTGAGAAGAAGAAATTTGTGA
>g15301.t3 Gene=g15301 Length=573
MGFVEETFYAMVRGITQSIINAHNNMVEGRIFLSETEIFDANINRSPLAYENNPDEEKAQ
YRDNVDKTLFQLRFMDKQNKKILGAFNWFAVHATSMNNTNKFISSDNVGYASMLLEKEYN
YDSMVGKGSFVGAFCSANLGDVSPNIMGPKCLSTGLPCDPLTSKCPNKDVCVASGPGRNI
YESTRIIGSRIYKGASKLLQVKGGREVTGPLSFVHQFIDMPKQTGVYFNPKLRGLQNYTG
CYPAMGYSFAAGTTDGPGAFDFAQGTTSENAFWNAVRDFIAEPSIQDIACHGSKPILIAT
GRAVYPYLWQPSVVPLQIFAIGDLLMFGLPGEFTTMAGRRLKNEIQLFAKSRSYEFNTIL
CGLSNIYTSYITTPEEYEIQRYEGASTIFGPHTLTIYINRFIKLLDAMLRGAAVDPGPMP
IDQDSKQISLVTKVYYDGHAIGSGFGYVVTQPKKSYRRGQIVTCKFVAGNPRNNFMTDSS
YFFVERLVSNGEWQMVATDADWETKFKWTRLSMILGRSEIEFMWEIPESTPSGEYRVRHQ
GYYQYILGGVYYYQGSTEHFNIFDDNNLRRRNL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g15301.t3 | Gene3D | G3DSA:2.60.40.2300 | - | 434 | 567 | 0 |
| 3 | g15301.t3 | PANTHER | PTHR12670:SF1 | NEUTRAL CERAMIDASE-RELATED | 1 | 562 | 0 |
| 4 | g15301.t3 | PANTHER | PTHR12670 | CERAMIDASE | 1 | 562 | 0 |
| 1 | g15301.t3 | Pfam | PF04734 | Neutral/alkaline non-lysosomal ceramidase, N-terminal | 1 | 399 | 0 |
| 2 | g15301.t3 | Pfam | PF17048 | Neutral/alkaline non-lysosomal ceramidase, C-terminal | 403 | 562 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046514 | ceramide catabolic process | BP |
| GO:0017040 | N-acylsphingosine amidohydrolase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
