General information for g15325.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable peroxisomal acyl-coenzyme A oxidase 1.
Parent gene
- The parent gene g15325 is located on chr_4: 4972900..4974055 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15325 | g15325.t2 | TTS | g15325.t2 | 4970594 | 4970594 |
| chr_4 | g15325 | g15325.t2 | isoform | g15325.t2 | 4970657 | 4972367 |
| chr_4 | g15325 | g15325.t2 | exon | g15325.t2.exon1 | 4970657 | 4970917 |
| chr_4 | g15325 | g15325.t2 | cds | g15325.t2.CDS1 | 4970657 | 4970917 |
| chr_4 | g15325 | g15325.t2 | exon | g15325.t2.exon2 | 4970972 | 4972367 |
| chr_4 | g15325 | g15325.t2 | cds | g15325.t2.CDS2 | 4970972 | 4972366 |
| chr_4 | g15325 | g15325.t2 | TSS | g15325.t2 | 4972973 | 4972973 |
Sequences
>g15325.t2 Gene=g15325 Length=1657
AATGTTTTTATTCATGATGTTTAAATTAACAAATGATGAACAAAAGGCTGAATGGCTTCC
TAAAGTTCTTAATTGCAATATTCTTGGCACTTATGCACAAACTGAACTTGGTCATGGAAC
AAATGTTAGAGGCTTGGAAACAACAGCAACCTATGATCCAATAACTAAAGAATTCATCAT
AAACACACCAAGTTTGTCAGCTTATAAATTTTGGCCAGGTAACATGGGTCATACTGTCAA
CTATGCAGTTGTTCTTGCACAATTGTATACTAAAGGTCAATGCTATGGAATTCAACCATT
TATTGTTCCAATTAGAGACTTAGAGACACATAAACCTCTACCAGGAATCACAGTTGGTGA
TATAGGAAATAAAATGGGCTACATGACTATGAACAATGGATATCTTGCATTCAACAATGT
TCGAATTCCTTTCAAAAATTTGCTTATGAGAAACGCAAAAATGCTTGAAGATGGAACTTT
TATTAAACCAAAAATTTCTGTACTTAATTATGGTGCTATGAGTTCAATGAGAAATTCAAT
TTTAAATGATACTGCAATGATGATGTCACGTGCTGCAACAATTGCTACTCGATATTCTTT
AGTCCGCCGACAGAGTCCGATAAATCCAAATGATCCCGAACCAAAAATTATCGAACATAA
AACCCAGCAGTATAAAGTTTTTGTGCCATTAGCAAAAGCAATCATTTATAGAATTGTTTC
AAGTTATCTTGATGAATTGTTTACCCAAATTCAACAGGAATCAAGTTCAGGAAATTTTGA
TAGATTGAATGAACTTCATGCATTAAATTGCGGTCTAAAAGCTGTGATGTCTGAAGAAGG
AACAAAAGCAATTGAACTTTGCAGACTTGCATGTGGCGGTCATGGTTATTTGAAATCAGC
TGGTTTTGATTCTATTTATCGCTTATGTACAGCTGCACAAACTGGTGAAGGTGAAAATAC
AGTGATGCATTTGCAAACAGCTCGTTATTTAATGAAAACATTCACAAAAGCAAGAAATGG
TGAGAAATTAACTGAAGGTGTTGCTTATTTGAGTGAATTTATCAACTTAAATGGTCGACG
TTTGATTTTTGATGATTCAATTCGAGGAATTTTGAGGGCAATTCAAACTGCAGCTGCTAG
TAAAATTGTAATTGCTTGGAAACACATTGAAGAAAGAAAGAAAAATTTCACAGCAGAAGA
AGCAGCCAGTTATACTGGAATTGAATTGACAAGAGCATCAGAACTTCATTGTCACACTTG
TTTGCTACAAATTGCAATAGTTGAAATTGAGAAAGCAATCGGAAAAGTTTCACGAGCATT
GGGAGAAGTTTTGAGAAATGTTTTGGAACTTTATGCTGTTGATACAATTTTGAAACTTTC
TGGTGATGTTTTATTGTTTGTTAACATGACAGCTGAAGATGTTGAAAAACTTCAAAAACG
TTTGGAAAATTCTTTACAATTTTTCAGAAGCACTGCAATTGGCATCGTTGATGGATTTGA
TTTTTCTGATCAAATTTTGGACTCCACACTTGGTGCATATGATGGAAATGTTTATGAAAA
TTTATTTGCTGCTACCAAAAAGTCAACATTGAATGACGAAGAAGTCAACAAAAGTTTCCA
TTTGTATTTGAAGCCATTTATGAAGGCAAATTTGTAA
>g15325.t2 Gene=g15325 Length=551
MFLFMMFKLTNDEQKAEWLPKVLNCNILGTYAQTELGHGTNVRGLETTATYDPITKEFII
NTPSLSAYKFWPGNMGHTVNYAVVLAQLYTKGQCYGIQPFIVPIRDLETHKPLPGITVGD
IGNKMGYMTMNNGYLAFNNVRIPFKNLLMRNAKMLEDGTFIKPKISVLNYGAMSSMRNSI
LNDTAMMMSRAATIATRYSLVRRQSPINPNDPEPKIIEHKTQQYKVFVPLAKAIIYRIVS
SYLDELFTQIQQESSSGNFDRLNELHALNCGLKAVMSEEGTKAIELCRLACGGHGYLKSA
GFDSIYRLCTAAQTGEGENTVMHLQTARYLMKTFTKARNGEKLTEGVAYLSEFINLNGRR
LIFDDSIRGILRAIQTAAASKIVIAWKHIEERKKNFTAEEAASYTGIELTRASELHCHTC
LLQIAIVEIEKAIGKVSRALGEVLRNVLELYAVDTILKLSGDVLLFVNMTAEDVEKLQKR
LENSLQFFRSTAIGIVDGFDFSDQILDSTLGAYDGNVYENLFAATKKSTLNDEEVNKSFH
LYLKPFMKANL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g15325.t2 | Gene3D | G3DSA:2.40.110.10 | - | 29 | 166 | 0 |
| 9 | g15325.t2 | Gene3D | G3DSA:1.20.140.10 | - | 168 | 341 | 0 |
| 10 | g15325.t2 | Gene3D | G3DSA:1.20.140.10 | - | 366 | 500 | 0 |
| 3 | g15325.t2 | PANTHER | PTHR10909:SF250 | ACYL-COENZYME A OXIDASE | 1 | 550 | 0 |
| 4 | g15325.t2 | PANTHER | PTHR10909 | ELECTRON TRANSPORT OXIDOREDUCTASE | 1 | 550 | 0 |
| 11 | g15325.t2 | PIRSF | PIRSF000168 | Acyl-CoA_oxidase | 1 | 551 | 0 |
| 1 | g15325.t2 | Pfam | PF02770 | Acyl-CoA dehydrogenase, middle domain | 31 | 140 | 0 |
| 2 | g15325.t2 | Pfam | PF01756 | Acyl-CoA oxidase | 369 | 548 | 0 |
| 5 | g15325.t2 | SUPERFAMILY | SSF56645 | Acyl-CoA dehydrogenase NM domain-like | 2 | 152 | 0 |
| 6 | g15325.t2 | SUPERFAMILY | SSF47203 | Acyl-CoA dehydrogenase C-terminal domain-like | 169 | 351 | 0 |
| 7 | g15325.t2 | SUPERFAMILY | SSF47203 | Acyl-CoA dehydrogenase C-terminal domain-like | 366 | 548 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016627 | oxidoreductase activity, acting on the CH-CH group of donors | MF |
| GO:0006635 | fatty acid beta-oxidation | BP |
| GO:0003997 | acyl-CoA oxidase activity | MF |
| GO:0006631 | fatty acid metabolic process | BP |
| GO:0005777 | peroxisome | CC |
| GO:0055114 | NA | NA |
| GO:0071949 | FAD binding | MF |
KEGG
Orthology
- This transcript has been annotated with K00232 (acyl-CoA oxidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed