General information for g15357.t1
Yuki Yoshida
6/2/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Estradiol 17-beta-dehydrogenase 8.
Parent gene
- The parent gene g15357 is located on chr_4: 5106427..5112827 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15357 | g15357.t1 | isoform | g15357.t1 | 5106427 | 5112827 |
| chr_4 | g15357 | g15357.t1 | TTS | g15357.t1 | 5106427 | 5106427 |
| chr_4 | g15357 | g15357.t1 | exon | g15357.t1.exon1 | 5106427 | 5107350 |
| chr_4 | g15357 | g15357.t1 | cds | g15357.t1.CDS1 | 5107337 | 5107350 |
| chr_4 | g15357 | g15357.t1 | exon | g15357.t1.exon2 | 5107404 | 5107973 |
| chr_4 | g15357 | g15357.t1 | cds | g15357.t1.CDS2 | 5107404 | 5107973 |
| chr_4 | g15357 | g15357.t1 | exon | g15357.t1.exon3 | 5112515 | 5112631 |
| chr_4 | g15357 | g15357.t1 | cds | g15357.t1.CDS3 | 5112515 | 5112631 |
| chr_4 | g15357 | g15357.t1 | exon | g15357.t1.exon4 | 5112710 | 5112827 |
| chr_4 | g15357 | g15357.t1 | cds | g15357.t1.CDS4 | 5112710 | 5112752 |
| chr_4 | g15357 | g15357.t1 | TSS | g15357.t1 | 5112827 | 5112827 |
Sequences
>g15357.t1 Gene=g15357 Length=1729
GTGTGTTTTGCTTCAAATTTTTATAAGAAAATTTTCGTTCTTCCGCTAATTTAAGTTTGA
TTTGCCGGTATCAAAATGTCTTCAACACTTTTATTGGGAAAATTAGCATTTGTGACAGGA
GCTGGAAGCGGAATTGGACGAGCGACAGCAATTCGTTTTGCTCAAGAAGGAGCAACAGTC
ATTGCTGCAGATAGAAATTTTAAACAAGCTGAAGAAACTGCCAAAAAACTTGGAGCAACA
CATCTTCCAATTGAAGTTGATGTAACAAATGCAGAAAGCATTAAAAATGTCATTGAAGAA
GCAATCAATAAATATAAAAGACCACCAACGACAATTGTCAATTGTGCTGGCATCACACGT
GATTCTTTCTTACTCAAAATGGATGACAGTGATTTTGATTTGGTGCTAAATGTTAATCTT
AAAGGCACATATCTTGTTATGAAACATTCAGTACAACGCATGATTGATTATAAAATTGGT
GGTTCAATAATTAACATTTCAAGCATCACAGCTCGTATGGGCAATATGGGTCAATGCAAT
TATGCACCATCAAAAGCTGCAGTTGAGACACTTTCACGAGTTGCTGCCAAAGAATTTGCT
CGATATGGAATTAGAGTTAACACTGTTATTCCTGGATTTATAAACACACCGATGACACAA
ACTGTACCAGATAAAGTAAAAGATATGGTCATTGGAAGCATCGCATTGAGACGATTTGGT
GAACCAGAAGAAGTCAGTGATACAATTGCATTTTTGGCTTCAGATAAAAGTTCATTCATT
ACTGGTGCATCAATTGAAGTTTCAGGTGGACAACAATAAAAATTCAAACCAAAAATATTT
AACGAGATGGAAAATATCAAAATTAAAAATTTACATTGTGAATCCATCAAAAGTGCTTCG
ATAAACGAACTTCGATTTTTATCAAAAGGAAATCTAATCATTTTTTTATAAATTTACACC
AGCATTCAAAAGCTTTTTATCACAGAATCAAATCACAACAGTATGATGAACTTTAACCTC
AATTTGTAATATTTTTGATTATCTACAAGCATTTACAAAAAGTATAAAGAAAAGAAAATC
TTTTTTGAAAATTATTTTTCAAGCATTTAATCAAGAAGTATAGAAATATAGAGCTTTTAT
GAAATGATACAAATTTTACATATTTATTTTGATTTTTGCCATTTTCATTTTTGAGATTTT
TTGTCAAAGATTTTTTTTGTTTTATTTTTTTTAAGTTTACTATTAAAAACAAATGATGAA
ATTTTAGAAAATAATAAAAAAGTGAAAAGAAAAACGAAAAATTTAAATTTTTTAGATAAC
TATGATATCTAAAATTATTTTTGTTTTTTGAATAGTTGTCTTTATTTGAGTTTTTAGTCA
ATTTTTTTAAATTCTAAATTTTTTTTAGTATTTTTAAAAACCTGTTCTAAAATTTTATTG
AAGTGTAATTGTCCATATCTATTATGCTAAAATACATGAAAAATATTGTTCATCATCAAA
GGCAATTCAAAATTTTGAGAATAATAAAGTTTTATGTATTTTAGCAGATAAAGTCAAAAA
ATTTAGAACATGTCTCAAATTAATTTTCAAAATTTTTAAAACAACAAACGATTATACAAA
TTTGGAACTACCTGATACATCAAATCAGTAACATAAAAATTAGCAATAAAAGAAATTTTT
TGTGCTTCATATTTGATAAAATCAATAAAAACATATTTTTTAAAATGAT
>g15357.t1 Gene=g15357 Length=247
MSSTLLLGKLAFVTGAGSGIGRATAIRFAQEGATVIAADRNFKQAEETAKKLGATHLPIE
VDVTNAESIKNVIEEAINKYKRPPTTIVNCAGITRDSFLLKMDDSDFDLVLNVNLKGTYL
VMKHSVQRMIDYKIGGSIINISSITARMGNMGQCNYAPSKAAVETLSRVAAKEFARYGIR
VNTVIPGFINTPMTQTVPDKVKDMVIGSIALRRFGEPEEVSDTIAFLASDKSSFITGASI
EVSGGQQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g15357.t1 | Gene3D | G3DSA:3.40.50.720 | - | 1 | 247 | 4.4E-83 |
| 2 | g15357.t1 | PANTHER | PTHR42760 | SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER | 4 | 245 | 7.6E-92 |
| 3 | g15357.t1 | PANTHER | PTHR42760:SF83 | ESTRADIOL 17-BETA-DEHYDROGENASE 8 | 4 | 245 | 7.6E-92 |
| 7 | g15357.t1 | PRINTS | PR00081 | Glucose/ribitol dehydrogenase family signature | 10 | 27 | 8.0E-35 |
| 5 | g15357.t1 | PRINTS | PR00080 | Short-chain dehydrogenase/reductase (SDR) superfamily signature | 82 | 93 | 1.2E-6 |
| 8 | g15357.t1 | PRINTS | PR00081 | Glucose/ribitol dehydrogenase family signature | 130 | 146 | 8.0E-35 |
| 6 | g15357.t1 | PRINTS | PR00080 | Short-chain dehydrogenase/reductase (SDR) superfamily signature | 136 | 144 | 1.2E-6 |
| 4 | g15357.t1 | PRINTS | PR00080 | Short-chain dehydrogenase/reductase (SDR) superfamily signature | 156 | 175 | 1.2E-6 |
| 9 | g15357.t1 | PRINTS | PR00081 | Glucose/ribitol dehydrogenase family signature | 156 | 175 | 8.0E-35 |
| 11 | g15357.t1 | PRINTS | PR00081 | Glucose/ribitol dehydrogenase family signature | 177 | 194 | 8.0E-35 |
| 10 | g15357.t1 | PRINTS | PR00081 | Glucose/ribitol dehydrogenase family signature | 210 | 230 | 8.0E-35 |
| 1 | g15357.t1 | Pfam | PF13561 | Enoyl-(Acyl carrier protein) reductase | 15 | 245 | 6.7E-64 |
| 16 | g15357.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 17 | g15357.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 18 | g15357.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 19 | g15357.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 23 | - |
| 15 | g15357.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 247 | - |
| 13 | g15357.t1 | SMART | SM00822 | This enzymatic domain is part of bacterial polyketide synthases and catalyses the first step in the reductive modification of the beta-carbonyl centres in the growing polyketide chain. It uses NADPH to reduce the keto group to a hydroxy group. | 9 | 192 | 0.0017 |
| 12 | g15357.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 8 | 246 | 6.6E-83 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
## [1] "No matching GO terms"KEGG
Orthology
- This transcript has been annotated with K13370 (17beta-estradiol 17-dehydrogenase / 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+) / 3-oxoacyl-[acyl-carrier protein] reductase alpha subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
