General information for g15371.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lysosomal thioesterase PPT2-like protein.
Parent gene
- The parent gene g15371 is located on chr_4: 5147630..5149830 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15371 | g15371.t3 | TSS | g15371.t3 | 5146872 | 5146872 |
| chr_4 | g15371 | g15371.t3 | isoform | g15371.t3 | 5146874 | 5149830 |
| chr_4 | g15371 | g15371.t3 | exon | g15371.t3.exon1 | 5146874 | 5147043 |
| chr_4 | g15371 | g15371.t3 | cds | g15371.t3.CDS1 | 5146900 | 5147043 |
| chr_4 | g15371 | g15371.t3 | exon | g15371.t3.exon2 | 5147281 | 5147530 |
| chr_4 | g15371 | g15371.t3 | cds | g15371.t3.CDS2 | 5147281 | 5147530 |
| chr_4 | g15371 | g15371.t3 | exon | g15371.t3.exon3 | 5147622 | 5147760 |
| chr_4 | g15371 | g15371.t3 | cds | g15371.t3.CDS3 | 5147622 | 5147760 |
| chr_4 | g15371 | g15371.t3 | exon | g15371.t3.exon4 | 5149227 | 5149336 |
| chr_4 | g15371 | g15371.t3 | cds | g15371.t3.CDS4 | 5149227 | 5149336 |
| chr_4 | g15371 | g15371.t3 | exon | g15371.t3.exon5 | 5149610 | 5149830 |
| chr_4 | g15371 | g15371.t3 | cds | g15371.t3.CDS5 | 5149610 | 5149830 |
| chr_4 | g15371 | g15371.t3 | TTS | g15371.t3 | 5149938 | 5149938 |
Sequences
>g15371.t3 Gene=g15371 Length=890
AGTAGTTTTTATTGAAATCAGCTGAAATGCATAAAAAAAATTTTATTTTTAAATTCTTAG
AATTTTTAATTTTGTTAACATGTTGTTGTGCTTATAAACCAGTGGTATTATTACATGGTA
TTCTTTCTGATGCTACTAGTATGATAATTATTGCAGAACGCATTAAATTGCATCATCCTG
GAACAGAAGTTTACTTAATTGACAAATTTAATAATTGGGGAAGTCTTGAAAATGCTTTAA
GTCAAATTGATTCATTTTTTGATGATTTTGACGAAATTTTCAAAAAGCATCCTGAAGGAG
TACACGTTCTTGGTTATTCACAAGGTGGACTTTTAGCCAGAGCTTTAATTCAATTTTATA
AAAATCACAATGTTAAGAAATTTATTTCACTGAGCAGTCCACAAGCTGGTCAATTTGGAT
CTGCTTTCCTTCATTTAATTTTTCCTACACTGGTTGCTAACAATGCTTATGAACTTTTTT
ATTCTCGTCTTGGCCAAAGAACTTCAGTTGGCAATTATTGGAATGATCCCCATCAAAGAC
CTTTGTATCTTAAATATAGTAATTTTCTACCTTATGTAAATAATGAAATCTTATGTACAA
ATTCTTCACAATTTCGTGATAATCTATTAAAGCTAGAAAAAATGATTCTCATTGGTGGTC
CTGATGATGTAATAACTCCATGGGAAAGCGCACATTTTGGTTTTTATGATCAAAATTTAA
CAGTAATACCTTTAAGAGAGCGACAATTTTATGTAGAAGATGCAATTGGTTTAAAGACTT
TAGACGAACAAAAGAAACTTGAACTTGTGACTGTTCCACATATTATACATATAGCATGGC
ACTTAAATGTAACGCTCATCGATGAAATCATCGTTCCTAGATTAGACTGA
>g15371.t3 Gene=g15371 Length=287
MHKKNFIFKFLEFLILLTCCCAYKPVVLLHGILSDATSMIIIAERIKLHHPGTEVYLIDK
FNNWGSLENALSQIDSFFDDFDEIFKKHPEGVHVLGYSQGGLLARALIQFYKNHNVKKFI
SLSSPQAGQFGSAFLHLIFPTLVANNAYELFYSRLGQRTSVGNYWNDPHQRPLYLKYSNF
LPYVNNEILCTNSSQFRDNLLKLEKMILIGGPDDVITPWESAHFGFYDQNLTVIPLRERQ
FYVEDAIGLKTLDEQKKLELVTVPHIIHIAWHLNVTLIDEIIVPRLD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g15371.t3 | Gene3D | G3DSA:3.40.50.1820 | - | 22 | 287 | 1.5E-81 |
| 2 | g15371.t3 | PANTHER | PTHR11247 | PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1 | 15 | 286 | 1.1E-100 |
| 3 | g15371.t3 | PANTHER | PTHR11247:SF27 | LYSOSOMAL THIOESTERASE PPT2 | 15 | 286 | 1.1E-100 |
| 1 | g15371.t3 | Pfam | PF02089 | Palmitoyl protein thioesterase | 25 | 265 | 6.7E-61 |
| 8 | g15371.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 9 | g15371.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 10 | g15371.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 17 | - |
| 11 | g15371.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 7 | g15371.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 287 | - |
| 4 | g15371.t3 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 23 | 272 | 5.17E-65 |
| 5 | g15371.t3 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016790 | thiolester hydrolase activity | MF |
| GO:0008474 | palmitoyl-(protein) hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01074 (palmitoyl-protein thioesterase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
