General information for g15412.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative GPI ethanolamine phosphate transferase 2.
Parent gene
- The parent gene g15412 is located on chr_4: 5365094..5366209 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15412 | g15412.t2 | isoform | g15412.t2 | 5365094 | 5366209 |
| chr_4 | g15412 | g15412.t2 | exon | g15412.t2.exon1 | 5365094 | 5365807 |
| chr_4 | g15412 | g15412.t2 | cds | g15412.t2.CDS1 | 5365095 | 5365807 |
| chr_4 | g15412 | g15412.t2 | exon | g15412.t2.exon2 | 5365882 | 5366209 |
| chr_4 | g15412 | g15412.t2 | cds | g15412.t2.CDS2 | 5365882 | 5366209 |
| chr_4 | g15412 | g15412.t2 | TSS | g15412.t2 | 5366967 | 5366967 |
| chr_4 | g15412 | g15412.t2 | TTS | g15412.t2 | NA | NA |
Sequences
>g15412.t2 Gene=g15412 Length=1042
ATGACAATAAAAAGTGAAAATTTCTATTTAGTTCTACTAACGGCAAGTGTCATTTCATCA
TTCTTTTTTTGTATTGGATTCTTTCCTTATTCGAATGTTAAAAAATTAAAATCAGAACTT
AGCAATAATGAATTGGAAAAGTTGAATTTACCAAAAATTAATCGATCAATTTTAATGCTG
ATTGATGCACTTCGACTAGACTTGATAAGTGATAGAAATAATTTTCCATTTGTGCATGAA
GTACTCAATAAAGGTGATGGATGTATGCTACAAATGAAGGTTAATCTCCCGACTGTTACT
AAACCAAGAATTACCGCAATGACATCAGGAACTGTTCCGTCATTTCTTGATGTAGCTAAA
AATTTTGCTAAAAATAAAATAAAAATTGACACTTTTCTTCATCAAATGAATGCAAGAAAT
CAAAAAATTGTCTTTGCCGGTGATGATACTTGGTTAATGTTTCCATTCTTTACTCGTCAC
TATGCAAATCGTGATTCACTTTTTGTCAATGATTTTTATGAAGGTGATTTAAATATTACA
AGATCTCTAGCTTATGAATTAAAACAAAATGATTGGAAATTGTTGATTCTTCATTATTTG
GGACTTGATCACATTGGACACGTCTTTAATCCTTTTCATGAATTAATTCAACCTAAATTG
CAAGAAATGGATCAAGTAATAAAAACGATTGTTGAAAAATTATCAGAATGGAATTCAAAA
AGTAAAGAAAAGTCAATTTTGATACTCACAAGTGACCATGGAATGAGAAATGCTGGAGGA
CATTCAGGAAGTTCTATCGCTGAAACTCACATTCCATTGTTAATTTTGGGTCACAATTGC
ACATCAAATGATAAAGAATTTTACAATCAAATTGATTTAGCAACGACTTTTTCAATCATG
AATGGACTACAAATTCCTGAGTCATCAATTGGTGCTGTGATTCCTGAATTATTATTTAAT
ATGGAACCTATTAAGCAATTGAATGTTGTGAAAATTATTAATGAAAGATTATTGAAAATG
ATTGAACATGACAAGAGTGAAG
>g15412.t2 Gene=g15412 Length=347
MTIKSENFYLVLLTASVISSFFFCIGFFPYSNVKKLKSELSNNELEKLNLPKINRSILML
IDALRLDLISDRNNFPFVHEVLNKGDGCMLQMKVNLPTVTKPRITAMTSGTVPSFLDVAK
NFAKNKIKIDTFLHQMNARNQKIVFAGDDTWLMFPFFTRHYANRDSLFVNDFYEGDLNIT
RSLAYELKQNDWKLLILHYLGLDHIGHVFNPFHELIQPKLQEMDQVIKTIVEKLSEWNSK
SKEKSILILTSDHGMRNAGGHSGSSIAETHIPLLILGHNCTSNDKEFYNQIDLATTFSIM
NGLQIPESSIGAVIPELLFNMEPIKQLNVVKIINERLLKMIEHDKSE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g15412.t2 | Gene3D | G3DSA:3.40.720.10 | Alkaline Phosphatase | 54 | 318 | 1.0E-39 |
| 2 | g15412.t2 | PANTHER | PTHR23072 | PHOSPHATIDYLINOSITOL GLYCAN-RELATED | 9 | 344 | 2.2E-97 |
| 1 | g15412.t2 | Pfam | PF01663 | Type I phosphodiesterase / nucleotide pyrophosphatase | 183 | 257 | 1.7E-7 |
| 6 | g15412.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 8 | g15412.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 30 | - |
| 7 | g15412.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 31 | 347 | - |
| 4 | g15412.t2 | SUPERFAMILY | SSF53649 | Alkaline phosphatase-like | 55 | 314 | 1.1E-34 |
| 3 | g15412.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006506 | GPI anchor biosynthetic process | BP |
| GO:0051377 | mannose-ethanolamine phosphotransferase activity | MF |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05310 (ethanolamine phosphate transferase 2 subunit G [EC:2.7.-.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
