General information for g15432.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase 116 kDa subunit a isoform 1.
Parent gene
- The parent gene g15432 is located on chr_4: 5433994..5436295 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15432 | g15432.t1 | TTS | g15432.t1 | 5432568 | 5432568 |
| chr_4 | g15432 | g15432.t1 | isoform | g15432.t1 | 5432674 | 5436295 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon1 | 5432674 | 5432936 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS1 | 5432674 | 5432936 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon2 | 5433017 | 5433149 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS2 | 5433017 | 5433149 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon3 | 5433212 | 5434594 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS3 | 5433212 | 5434594 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon4 | 5434649 | 5434849 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS4 | 5434649 | 5434849 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon5 | 5434909 | 5435202 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS5 | 5434909 | 5435202 |
| chr_4 | g15432 | g15432.t1 | exon | g15432.t1.exon6 | 5436179 | 5436295 |
| chr_4 | g15432 | g15432.t1 | cds | g15432.t1.CDS6 | 5436179 | 5436295 |
| chr_4 | g15432 | g15432.t1 | TSS | g15432.t1 | 5436413 | 5436413 |
Sequences
>g15432.t1 Gene=g15432 Length=2391
ATGGGTGCACTTTTTAGAAGTGAAGAAATGTCTTTATGCCAGCTGTTTATACAGCCTGAA
GCAGCATACACAACTGTAGCTGAACTTGGTGAAATTGGAATTGCACAATTTCGTGATCTT
AATCCAGAACTTTCAGCTTTTCAACGTAAATTCACAGCTGAAGTTCGTCGTTGTGATGAA
ATGGAAAGAAAAGTTAATTATATTAAAAAAGAATTGATAAAAGATGGAATGGAAGTTAAA
GATCTTTTTTATAATCTTCCACCTGTTCCTAATCCTCGAGAATTTATCGATCTTGAAGCA
CTTTTTGAGAAAACTGAAAATGAAATTTTGGAATTATCTGAGAATTTCAATCAATTATTG
CAGAATTTTCAAGAACTAACTGAACTTCGTTATGTTCTCGAAAGAACTCAATCATTCTTT
TCTGAGCATGTTTCAAACTATGAAGGATCAAAAGCTGAGGAAGCAGGTGAAACCCAACAG
CTTGGCTTTGTTGCTGGTGTAATTGAGCGTGAAAAAGTATTGGGTTTTGAACGCATTCTT
TGGCGTATTTCACGTGGTAATGTTTTCTTGAGACATGCTGACATTGAGACGCCTTTTACT
GATCCTAAGACTGGAAGAGAACATCATAAGAATGTTTTTGTTGCATTCTTCCAAGGTGAT
CAATTGAAGACACGTGTAAAGAAAGTTTGTGCTGGTTATCATGCTTCGCTTTATCCATGT
CCAAATAATTATGAAGAACGTGATGATATGCTTAAAGGTGTAATGACTCGTCTTCAAGAT
TTAACTATGGTCATCAATCAAACCAAAGATCAACGTCAACGTGTTCTTGTTAGTGTTTCA
AAAGATCTTCCAAAATGGATAATAATTGTGAAGAAAATCAAGGCAATTTATCATACAATG
AATTTATTTAATATTGATGTTTCTGGAAAATGCTTAATTGGTGAATGTTGGATTCCAACA
AATGATTTACATTTGGTTCAATCAGCATTGACAAATGGATCAAATGTAGTTGGTTCAACT
GTTCCATCGTTCATGAATGTCATTGCAACCAATGAAAATCCACCAACATTTAATCGAACA
AATCGTTTCACTCAAGGTTTCCAGAACTTGATCGATGCTTTTGGAGTTGCAAATTATCGT
GAAGCAAATCCTGCACTTTACACAATTATCACTTTTCCATTTTTATTTGCGATTATGTTT
GGTGATTTGGGACATGGAATTATTATGTTTTTATTTGGTTTATGGATGGTCACAAGTGAG
AAAAAGTTGTTGATGCAAAAATCGAAGAATGAAATTTGGAACATCTTCTTTGGCGGACGC
TATATAATTTTGCTCATGGGATTTTTCTCAATGTACACTGGACTGATTTATAATGATTGC
TTTTCAAAGACAATGAACATTTTTGGATCATCATGGAAAATTGCATACAATGAAAGCACA
ATTATGAATAATACAGAAGGGCAATTACAATTGAATCCTAGTGATGATTCTTTGGTCGAA
GGTCGAACTTATCCATTTGGACTTGATCCAGCTTGGATGCTTGCAACAAACAAAATTATA
TTTTTGAACTCATTCAAAATGAAAGTTTCGATAATTTTTGGTGTTGTTCATATGATTTTT
GGTGTCATCATGTCAGTTGTCAATTACAATCACTTCCGTCGTAGAAGTTCAATTATTCTT
GAATTTTTACCACAAATTCTCTTCTTGGTTCTTTTATTCGCTTACATGGTCTTTATGATG
TTCTGGAAATGGTTCCGTTTTTCACCAAAATCACAGCCACCATATTCACCAGGATGTGCA
CCGTCGGTTTTGATTTATTTCATTAATATGATGCTGTTTGGTGAAAATAAATATCCTGAT
GGTTGTGAAGAATACATGTTCCCTTATCAACAAGAAGTTCAACTTTATTTTGTAATTATT
GCACTTTTATGCATTCCATGGATGTTGCTTGGAAAACCATTATTTATTATGTGTACTGGT
GGCAACAAAGGACATGGAGATCATGGAGATGAACCAATGAGTGAAATTTGGATTCATCAA
GCAATTCATACAATTGAATATGTTTTGAATACAATTTCACATACTGCTTCTTATCTTCGT
TTATGGGCTTTGTCACTTGCTCATGCTCAACTTTCTGAAGTCTTATGGGGAATGGTCTTA
AATATTGGCCTAACACAATCACTTTATGTTGGTGGTGCAATTCTTTTCCCAATTTTTGGT
GCTTGGGCTGTTTTGACTTTGGCAATTTTAGTCATGATGGAAGGATTGTCAGCATTTTTG
CACACTTTACGTCTTCATTGGGTTGAATTTATGAGCAAGTTCTACACTGGAACTGGCTAC
ATTTTTGCTCCATTCCATTTCAAGACAATTTTGAATGAAGAAGAAGATTAA
>g15432.t1 Gene=g15432 Length=796
MGALFRSEEMSLCQLFIQPEAAYTTVAELGEIGIAQFRDLNPELSAFQRKFTAEVRRCDE
MERKVNYIKKELIKDGMEVKDLFYNLPPVPNPREFIDLEALFEKTENEILELSENFNQLL
QNFQELTELRYVLERTQSFFSEHVSNYEGSKAEEAGETQQLGFVAGVIEREKVLGFERIL
WRISRGNVFLRHADIETPFTDPKTGREHHKNVFVAFFQGDQLKTRVKKVCAGYHASLYPC
PNNYEERDDMLKGVMTRLQDLTMVINQTKDQRQRVLVSVSKDLPKWIIIVKKIKAIYHTM
NLFNIDVSGKCLIGECWIPTNDLHLVQSALTNGSNVVGSTVPSFMNVIATNENPPTFNRT
NRFTQGFQNLIDAFGVANYREANPALYTIITFPFLFAIMFGDLGHGIIMFLFGLWMVTSE
KKLLMQKSKNEIWNIFFGGRYIILLMGFFSMYTGLIYNDCFSKTMNIFGSSWKIAYNEST
IMNNTEGQLQLNPSDDSLVEGRTYPFGLDPAWMLATNKIIFLNSFKMKVSIIFGVVHMIF
GVIMSVVNYNHFRRRSSIILEFLPQILFLVLLFAYMVFMMFWKWFRFSPKSQPPYSPGCA
PSVLIYFINMMLFGENKYPDGCEEYMFPYQQEVQLYFVIIALLCIPWMLLGKPLFIMCTG
GNKGHGDHGDEPMSEIWIHQAIHTIEYVLNTISHTASYLRLWALSLAHAQLSEVLWGMVL
NIGLTQSLYVGGAILFPIFGAWAVLTLAILVMMEGLSAFLHTLRLHWVEFMSKFYTGTGY
IFAPFHFKTILNEEED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g15432.t1 | Coils | Coil | Coil | 95 | 129 | - |
| 3 | g15432.t1 | PANTHER | PTHR11629 | VACUOLAR PROTON ATPASES | 1 | 664 | 0.0 |
| 5 | g15432.t1 | PANTHER | PTHR11629:SF61 | V-TYPE PROTON ATPASE SUBUNIT A | 1 | 664 | 0.0 |
| 4 | g15432.t1 | PANTHER | PTHR11629 | VACUOLAR PROTON ATPASES | 663 | 795 | 0.0 |
| 6 | g15432.t1 | PANTHER | PTHR11629:SF61 | V-TYPE PROTON ATPASE SUBUNIT A | 663 | 795 | 0.0 |
| 31 | g15432.t1 | PIRSF | PIRSF001293 | ATP6V0A1 | 1 | 665 | 1.1E-228 |
| 30 | g15432.t1 | PIRSF | PIRSF001293 | ATP6V0A1 | 658 | 796 | 2.3E-66 |
| 2 | g15432.t1 | Pfam | PF01496 | V-type ATPase 116kDa subunit family | 28 | 661 | 1.6E-240 |
| 1 | g15432.t1 | Pfam | PF01496 | V-type ATPase 116kDa subunit family | 664 | 787 | 3.3E-63 |
| 21 | g15432.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 393 | - |
| 28 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 394 | 417 | - |
| 17 | g15432.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 418 | 437 | - |
| 26 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 438 | 457 | - |
| 22 | g15432.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 458 | 530 | - |
| 25 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 531 | 550 | - |
| 16 | g15432.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 551 | 561 | - |
| 24 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 562 | 582 | - |
| 20 | g15432.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 583 | 593 | - |
| 29 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 594 | 613 | - |
| 18 | g15432.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 614 | 632 | - |
| 27 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 633 | 650 | - |
| 19 | g15432.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 651 | 727 | - |
| 23 | g15432.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 728 | 751 | - |
| 15 | g15432.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 752 | 796 | - |
| 7 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 393 | 415 | - |
| 13 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 436 | 458 | - |
| 12 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 529 | 551 | - |
| 11 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 558 | 580 | - |
| 9 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 595 | 614 | - |
| 10 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 635 | 657 | - |
| 8 | g15432.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 729 | 751 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0033179 | proton-transporting V-type ATPase, V0 domain | CC |
| GO:1902600 | proton transmembrane transport | BP |
| GO:0000220 | vacuolar proton-transporting V-type ATPase, V0 domain | CC |
| GO:0015078 | proton transmembrane transporter activity | MF |
KEGG
Orthology
- This transcript has been annotated with K02154 (V-type H+-transporting ATPase subunit a)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed