General information for g15469.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g15469 is located on chr_4: 5558607..5563069 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15469 | g15469.t1 | isoform | g15469.t1 | 5558607 | 5563069 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon1 | 5558607 | 5558713 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS1 | 5558607 | 5558713 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon2 | 5559002 | 5559556 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS2 | 5559002 | 5559556 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon3 | 5559612 | 5560082 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS3 | 5559612 | 5560082 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon4 | 5560183 | 5560827 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS4 | 5560183 | 5560827 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon5 | 5560885 | 5561014 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS5 | 5560885 | 5561014 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon6 | 5561199 | 5561362 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS6 | 5561199 | 5561362 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon7 | 5561484 | 5561735 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS7 | 5561484 | 5561735 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon8 | 5561982 | 5562166 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS8 | 5561982 | 5562166 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon9 | 5562411 | 5562605 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS9 | 5562411 | 5562605 |
| chr_4 | g15469 | g15469.t1 | exon | g15469.t1.exon10 | 5562657 | 5563069 |
| chr_4 | g15469 | g15469.t1 | cds | g15469.t1.CDS10 | 5562657 | 5563069 |
| chr_4 | g15469 | g15469.t1 | TSS | g15469.t1 | NA | NA |
| chr_4 | g15469 | g15469.t1 | TTS | g15469.t1 | NA | NA |
Sequences
>g15469.t1 Gene=g15469 Length=3117
ATGAATTTCATCAGTTTTATAAAAAATGTTCTCATTAACTTTTGTGCTAATACAAATATT
CATGGCTTCAGCTATATTGTTCAGTCAAGAAGACATTTTATTGAGAGGCTTTTCTGGATC
ATTTGCGTTACAATTTCGTTCGTTCTTACTGGAATTTTAATTAACAAACTGCTAATAGAA
AATCAACGAAATCCAACAATTATTTATACTGATCAAAATGTTGTTCATGTCACTGATATT
GATTTTCCATCTGTTACTTTTATGCCAGGCTTAATACTCAAAACTCCTTTGTATAATGGT
TTCAATTATGAATATTTCAAAAAAATATCATCAGTAACTGAAAAGTTTAGTTTTAAAAAA
TGGCATCCGGCATCATTTCATAGACTTCAAATTGCCAGCTTAATTGCTCGTGATGGATAT
GTTCGGAATAAATTTAAAATTTCAGTTTCAACTCATGACATTGTCAAACTGATGCGAAAA
GTTTTTCCATCATTTTGGTACACTCATCAAATTCATGAAATGTTTCCAAATGTTCCACAT
TATGCAACTTCATTGTGGGCTAACAAATACACGATAAATTTAACTGAAATTTTATGTCAA
ACTGGTTTTTGCTTTACTTTTAATTTTCCTAGTAAAATTGAAAATTTTTTCAAAATTCAT
AGATTATCAAAAGATTTCAATTTTACAAATTTTTGGCGAATCAAAAGAGAAAGAAAAGCA
ATTGCAGAAAATTACAAGAATGACTTTTCACTTTCCTATGGCGAATGTCCATTGAAAGGT
CCAAGAAATCGCAAAGGTTTGCTTTTTACTCACAATCAAATTTACATGCATCCAAATGGA
TGTTTAAGTCACATTTTTAAAAATAAGAAAAAACTCAATAATGAAAATTATTATGAAAAT
TTAAAAGACGTTGTTTACAAAATGATTGATAATCACATTAAGTATGACATTTTTGAGCAT
ACTGTACCAAAGCAGGATTATTTTGGACTACCTTTTGTCATTCACTCACCATATGAAGTT
ATTAGTGATCAATTAATGCAATTTAATTTACTTAGAACTTATAGAACAACAATTTTGGTA
ACACCGAGGATTACAAAACATGATGCATCACTAGCTGAGCTAAGCATTGATGAACGACAA
TGTTACTTATCAAATGAGAAAAAATTAAGATTCTTTAACATTTACAATCGAGTCAACTGT
GAACATGAATGTTTAGCAAGAATGACATTAAAATCATGCAATTGCACACAATTTTACATG
GTAAGAAGTAAGAAAACAAGAATTTGTGGACTTGGGGATGAAAAATGTTTTTGGAAAATT
GAAAGTAATTTTCAATCAGTAAAATCAAAATGTAAATGTTACGAACTTTGTGAAAGAATT
GAATATGATGTGAAAATGGATGTTCAAAAAATCATACCAAAGAAGGTTCTTTACATAAAA
GAACATAAATCGTTTTTGGAAGTGAATTTTGCTGTTGATCGGGTTTTAGCAATCACAAGA
AGCAAACAACAAACTTTAGTTGGATTCTTTTCATTAACTGGTGGCTTCTTGGGACTTTTT
GCTGGATTTTCAGTTCTTTCTAAATTTGAATTTTTTTACTTTTTATTTTTTGAGCCGATT
TTGAAAGTTTTGAAGAAAAATGACAATCGAGTTTATACAATGAAAGAAGGAAACACAAAA
AATAAAATTGTTTGGAAATATTTGAGACAATTTCTTAATGATTCTTCAATTCATGGTTTT
AATCACATTGGAAATGATCAAAAATTTATTATTGAAAGAACTGTTTGGTTGATTTTATTT
CTATTGGCAATGACTGGTTCTTATTTTATGGTTATTCAAGTTTACAAAACACTTCAAATT
GATCAAGTTTTAATGACAACTGAAGGACTTGTGACAAAATCTGATGAAGTTCCATTTCCA
GCAATAACTTTCACTGATTTGGTGATTCTAAGCTTCTATATGTTTTCTTATGTTGCTCAT
TCGTCTGAACTCGAAGATTTTACTAATTTTTTAGCAAAAGCTCCAATTGAGAAGTTTAAC
AGAAATGTTTCGGAATCAATAAATACATTTGATTATCAAATGTATTCAATAATTTGTCGC
AATCGATATTCAGAGCAGCTTCGAAATTGGAATTATGAATTTTTCAATAGTTCACAACTT
GTAGATGAATTGCAAAATGAAATACCAATAGATAACTGGTATGATTGGCAAAATTCAACA
TGGAATGTAAATCAGACTGCTTTTTATGCTGAAACATTGACAATGAGAGGAATTGGTTAC
AGTTTTAATATGTTTGAAGAAGAGAAATTGTTGAAATTTAAGAAACTGACATCAGATTAT
CATTATAAATTTCCAAAATTACTTTCAAAAAAATCTTATCCTTGGTCAACATCAGCTGGT
GATCAAAAAGGATTGGAAGTTGAATTTTTTGGTTACAATGCAATAAACTTTGAATCTTCT
TGTGTGCAACCAGCATTTGCTATTCATGGATCAAATGAATTGCCTTATGGTTCAAAATTT
CGCAATATTGGCTACAGTACATCATATGACATTTTAATTACACTAGAAATAATTAAATCT
GATGAAGGTTTGCAAACAATCCCAATCAATGTAAGAAAATGTGTTTTTCAAGGCGAAAAA
AAGTTGAAATATTTCAAAATTTATACACAAAAAAATTGTGAAATAGAATGTTTGTCAAGA
TTAGCTTATTATGATTGTGGTTGTGTTCCTTTCTATTTTGTTCGTAATCAAACCATGAAA
ATTTGTGACTTTAAACAAATTTGGTATTGTGCATTTCGTTATGAGGAAACAAATTATGGA
AATGATGAAACTTGTAGATGTTTTCCTCTTTGTAATTCAGTTAGCTATAGCTATGAAATC
AATGAATTTCATAATGTAAACAAATCAGAAGACCATATGACATTGACTTTTAGATACAAA
GATAATGAGCATCTTGCACTTCTTCGCTATCAACCTTTCAAATTGGTCGATTTCTTATCT
TATGTGGGAAGCATTTTGGGATTATTTGCTGGAATTTCTGTTTTATCAATTGTTGAAATT
TTTTATTATTTTGTGTTGAGAATTTTGAGTAATATTTTGAAATTTCATGTTGATTAA
>g15469.t1 Gene=g15469 Length=1038
MNFISFIKNVLINFCANTNIHGFSYIVQSRRHFIERLFWIICVTISFVLTGILINKLLIE
NQRNPTIIYTDQNVVHVTDIDFPSVTFMPGLILKTPLYNGFNYEYFKKISSVTEKFSFKK
WHPASFHRLQIASLIARDGYVRNKFKISVSTHDIVKLMRKVFPSFWYTHQIHEMFPNVPH
YATSLWANKYTINLTEILCQTGFCFTFNFPSKIENFFKIHRLSKDFNFTNFWRIKRERKA
IAENYKNDFSLSYGECPLKGPRNRKGLLFTHNQIYMHPNGCLSHIFKNKKKLNNENYYEN
LKDVVYKMIDNHIKYDIFEHTVPKQDYFGLPFVIHSPYEVISDQLMQFNLLRTYRTTILV
TPRITKHDASLAELSIDERQCYLSNEKKLRFFNIYNRVNCEHECLARMTLKSCNCTQFYM
VRSKKTRICGLGDEKCFWKIESNFQSVKSKCKCYELCERIEYDVKMDVQKIIPKKVLYIK
EHKSFLEVNFAVDRVLAITRSKQQTLVGFFSLTGGFLGLFAGFSVLSKFEFFYFLFFEPI
LKVLKKNDNRVYTMKEGNTKNKIVWKYLRQFLNDSSIHGFNHIGNDQKFIIERTVWLILF
LLAMTGSYFMVIQVYKTLQIDQVLMTTEGLVTKSDEVPFPAITFTDLVILSFYMFSYVAH
SSELEDFTNFLAKAPIEKFNRNVSESINTFDYQMYSIICRNRYSEQLRNWNYEFFNSSQL
VDELQNEIPIDNWYDWQNSTWNVNQTAFYAETLTMRGIGYSFNMFEEEKLLKFKKLTSDY
HYKFPKLLSKKSYPWSTSAGDQKGLEVEFFGYNAINFESSCVQPAFAIHGSNELPYGSKF
RNIGYSTSYDILITLEIIKSDEGLQTIPINVRKCVFQGEKKLKYFKIYTQKNCEIECLSR
LAYYDCGCVPFYFVRNQTMKICDFKQIWYCAFRYEETNYGNDETCRCFPLCNSVSYSYEI
NEFHNVNKSEDHMTLTFRYKDNEHLALLRYQPFKLVDFLSYVGSILGLFAGISVLSIVEI
FYYFVLRILSNILKFHVD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g15469.t1 | Gene3D | G3DSA:1.10.287.770 | - | 593 | 1021 | 3.4E-26 |
| 13 | g15469.t1 | Gene3D | G3DSA:2.60.470.10 | - | 623 | 991 | 3.4E-26 |
| 14 | g15469.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 648 | 823 | 3.4E-26 |
| 15 | g15469.t1 | Gene3D | G3DSA:1.10.287.820 | - | 866 | 951 | 3.4E-26 |
| 3 | g15469.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 7 | 552 | 6.8E-88 |
| 5 | g15469.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 7 | 552 | 6.8E-88 |
| 4 | g15469.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 562 | 1032 | 6.8E-88 |
| 6 | g15469.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 562 | 1032 | 6.8E-88 |
| 1 | g15469.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 14 | 534 | 1.6E-49 |
| 2 | g15469.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 571 | 1023 | 3.5E-49 |
| 19 | g15469.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 36 | - |
| 24 | g15469.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 59 | - |
| 20 | g15469.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 60 | 505 | - |
| 23 | g15469.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 506 | 526 | - |
| 18 | g15469.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 527 | 594 | - |
| 25 | g15469.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 595 | 615 | - |
| 21 | g15469.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 616 | 997 | - |
| 22 | g15469.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 998 | 1025 | - |
| 17 | g15469.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1026 | 1038 | - |
| 12 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
| 10 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 59 | - |
| 9 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 505 | 527 | - |
| 11 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 594 | 616 | - |
| 7 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 637 | 659 | - |
| 8 | g15469.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1004 | 1026 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
