General information for g15527.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Antitrypsin.
Parent gene
- The parent gene g15527 is located on chr_4: 5770995..5772501 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15527 | g15527.t1 | isoform | g15527.t1 | 5770995 | 5772501 |
| chr_4 | g15527 | g15527.t1 | exon | g15527.t1.exon1 | 5770995 | 5771258 |
| chr_4 | g15527 | g15527.t1 | cds | g15527.t1.CDS1 | 5770995 | 5771258 |
| chr_4 | g15527 | g15527.t1 | exon | g15527.t1.exon2 | 5771310 | 5771896 |
| chr_4 | g15527 | g15527.t1 | cds | g15527.t1.CDS2 | 5771310 | 5771896 |
| chr_4 | g15527 | g15527.t1 | exon | g15527.t1.exon3 | 5771954 | 5772167 |
| chr_4 | g15527 | g15527.t1 | cds | g15527.t1.CDS3 | 5771954 | 5772167 |
| chr_4 | g15527 | g15527.t1 | exon | g15527.t1.exon4 | 5772327 | 5772427 |
| chr_4 | g15527 | g15527.t1 | cds | g15527.t1.CDS4 | 5772327 | 5772427 |
| chr_4 | g15527 | g15527.t1 | exon | g15527.t1.exon5 | 5772486 | 5772501 |
| chr_4 | g15527 | g15527.t1 | cds | g15527.t1.CDS5 | 5772486 | 5772501 |
| chr_4 | g15527 | g15527.t1 | TSS | g15527.t1 | NA | NA |
| chr_4 | g15527 | g15527.t1 | TTS | g15527.t1 | NA | NA |
Sequences
>g15527.t1 Gene=g15527 Length=1182
ATGTCGATAATATTTGTTTTTACAACTTATTTATTTTTTTTACATCATTACTTTGTTGAT
GCACAAGATCAAACGTATTTACAAGCACAACAAAAATTTTCTCTTGAACTTTTTTCAAAT
GTCTCTGGTCCTGATTATTCTGCTGGTGTAATTCTATCACCAATAATTATTGACTTTTCT
CTTTCGCTTTTACTAATTGGTTCAAGCGGTAACACATTTAAACAACTTTTATTTGGTCTT
CAATATCCTGCAAATTACAGTATTAATTTAATTGAAATAAATTCAAATTTAATGATAAAA
AATATGATAAATGCTGGTGGAATTGAATATGGTATCAAAATCTATGTTGATAAAAAACTT
TCACTGCAAAATAATTTTAAAACAGCAATTCAAAAATATTTTAATTCTGGCATTGAGACA
GTTGATTTTGCTCAAAAATTTCAATCAGTTCAAACAATTAATGATTTCATATCAAATTCA
ACTCATGGAATGATTCAAAATGTCATGACTCCTGATGAATTGGATAGTCAAACTTCAATG
ATTCTTGTGAATTGCATTTATTTTAAAGGCGCTTGGGTTAATCCTTTTAATAAAAATAAC
ACAGAACAAATGGATTTCAAATTAGATAAAATTAATACAACAAAAATTGATTTTATGAAA
ATTAAAACAGATTTTAAATATGGAGCTGTTGATACTCTAAATGGTGCAAAAGTTGTTGAA
CTTCCTTATGTTAATTCAAGTGTTACTTTGACATTGATTTTACCACAAGAAGTTGATGGA
TTAAAAAATTTAATAACAGCAGCAAGAAATTACGATTGGTCGAAACTTTCGGAGAATTTA
AAAAATAAAAAAGTTTTGCTCATTATGCCAAAATTTAATGCAACTTTTGGACTTAATTTG
AATCAAGCAATTGAAAACATGGGAATGGATTCAATTTTTTATCCGTCAATTGCAAACATT
GATCGTGTTGCTCATAAAAATGGAAAACCATATTTAATAAGTTTGGAAAAAATGTATCAT
AAAGTCATTATTAGTGTTGATGAATATGGAACAACAGCAGCTGCTGTTAGTACTGCAAAT
AAGATTTCAACACCAGTAGTTTTCCAAGCTAATCAACCATTTTTATATGCTTTAAGATCA
AATTCAACAATTTATTTTATTGGACAATTTACTGGAAAATAA
>g15527.t1 Gene=g15527 Length=393
MSIIFVFTTYLFFLHHYFVDAQDQTYLQAQQKFSLELFSNVSGPDYSAGVILSPIIIDFS
LSLLLIGSSGNTFKQLLFGLQYPANYSINLIEINSNLMIKNMINAGGIEYGIKIYVDKKL
SLQNNFKTAIQKYFNSGIETVDFAQKFQSVQTINDFISNSTHGMIQNVMTPDELDSQTSM
ILVNCIYFKGAWVNPFNKNNTEQMDFKLDKINTTKIDFMKIKTDFKYGAVDTLNGAKVVE
LPYVNSSVTLTLILPQEVDGLKNLITAARNYDWSKLSENLKNKKVLLIMPKFNATFGLNL
NQAIENMGMDSIFYPSIANIDRVAHKNGKPYLISLEKMYHKVIISVDEYGTTAAAVSTAN
KISTPVVFQANQPFLYALRSNSTIYFIGQFTGK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g15527.t1 | CDD | cd00172 | serpin | 29 | 389 | 6.83616E-85 |
| 6 | g15527.t1 | Gene3D | G3DSA:3.30.497.10 | Antithrombin | 50 | 343 | 1.1E-85 |
| 5 | g15527.t1 | Gene3D | G3DSA:2.30.39.10 | - | 188 | 388 | 1.1E-85 |
| 2 | g15527.t1 | PANTHER | PTHR11461 | SERINE PROTEASE INHIBITOR, SERPIN | 28 | 391 | 3.8E-58 |
| 3 | g15527.t1 | PANTHER | PTHR11461:SF180 | LEUKOCYTE ELASTASE INHIBITOR | 28 | 391 | 3.8E-58 |
| 1 | g15527.t1 | Pfam | PF00079 | Serpin (serine protease inhibitor) | 29 | 390 | 7.7E-80 |
| 9 | g15527.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 10 | g15527.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 11 | g15527.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 13 | g15527.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 21 | - |
| 8 | g15527.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 44 | - |
| 12 | g15527.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 45 | 66 | - |
| 7 | g15527.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 67 | 393 | - |
| 15 | g15527.t1 | ProSitePatterns | PS00284 | Serpins signature. | 368 | 378 | - |
| 16 | g15527.t1 | SMART | SM00093 | serpin2 | 35 | 393 | 5.2E-66 |
| 4 | g15527.t1 | SUPERFAMILY | SSF56574 | Serpins | 10 | 391 | 2.88E-83 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005615 | extracellular space | CC |
| GO:0004867 | serine-type endopeptidase inhibitor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed