General information for g15528.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serpin B9.
Parent gene
- The parent gene g15528 is located on chr_4: 5772886..5774700 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15528 | g15528.t1 | isoform | g15528.t1 | 5772886 | 5774700 |
| chr_4 | g15528 | g15528.t1 | exon | g15528.t1.exon1 | 5772886 | 5773155 |
| chr_4 | g15528 | g15528.t1 | cds | g15528.t1.CDS1 | 5772886 | 5773155 |
| chr_4 | g15528 | g15528.t1 | exon | g15528.t1.exon2 | 5773212 | 5773798 |
| chr_4 | g15528 | g15528.t1 | cds | g15528.t1.CDS2 | 5773212 | 5773798 |
| chr_4 | g15528 | g15528.t1 | exon | g15528.t1.exon3 | 5773937 | 5774150 |
| chr_4 | g15528 | g15528.t1 | cds | g15528.t1.CDS3 | 5773937 | 5774150 |
| chr_4 | g15528 | g15528.t1 | exon | g15528.t1.exon4 | 5774456 | 5774562 |
| chr_4 | g15528 | g15528.t1 | cds | g15528.t1.CDS4 | 5774456 | 5774562 |
| chr_4 | g15528 | g15528.t1 | exon | g15528.t1.exon5 | 5774685 | 5774700 |
| chr_4 | g15528 | g15528.t1 | cds | g15528.t1.CDS5 | 5774685 | 5774700 |
| chr_4 | g15528 | g15528.t1 | TSS | g15528.t1 | NA | NA |
| chr_4 | g15528 | g15528.t1 | TTS | g15528.t1 | NA | NA |
Sequences
>g15528.t1 Gene=g15528 Length=1194
ATGGAGTGTTTTAAAGAATTCTTAATAATTTATGCAGCAGTTTTTCACTCTATTCATTTT
GTAAAAACACAAGATGTTGGTTTTTTAAACGCTCAGCAAAAATTTGCTCTTGAACTATTT
TCAACTATCTCAGGCCCAGATCATTCAACTGATGTAATTTTATCACCAATCTCTGTTAGT
ACTGCCCTTTCTATTCTCCTTATTGGATCAAATGGTGTTACATTTAAACAACTTTTCTTT
GGCCTTCAATATCCAACTAATTATAGCATCAATCTAGTTCAAGTTAATTCAAATTTGATG
ATTAAAAATATGGCAAAAGTTGGTGCAATTGAGTTTGCAACAAAACTTTTCATCACCAAA
AGTTTTTCACTTCAAAATAATTTTAAAACATCAATTGATAAATATTTTCAATCAGGTGTT
GAATCAGTTGATTTTTCCCAAAATGTTAAAGCAGCACAAAGAATTAATGATTATATTTCC
ACTTCAACACATGGAATGATTCAAGATGTCATAAAATCAGATGATTTAACCGAATATACA
ACAATGATTCTTGTAAATTGTATTTTCTTCAAAGGCTTATGGCAATTTCCATTTGATAAA
AGTTACACATTTAAAAGTGATTTCAACATTGATAACAAAAATACTATCCAAGTTGATTAT
ATGTATCAGGAAAATTCTTTTAATTTTGGCTACATCAATGCTTTAAATGATGCACAAGTT
CTCGAACTTCCGTATGCAAATTCTAAACTTACCTTGACACTCATTTTACCTCAAACAATT
GATGGGATAAAAAATTTGGTGAAAGCAGCTAAAAATTTTGATTTTACTAAACTTTCTAAT
CAATTAACAAACACTAGTGTCATTGTGTCAATTCCTAAATTTAATACAACTTTTGAACAA
TATTTAAATAGTCCTCTTACAAATATGGGAATGCCTTTACTTTTTAATAAAACAGTATCA
CAGCTTGATCATATTGCACATGAATTTACTGTTCCAAAGTCGTTAGCACTTGATGGACTT
TACCATAAAGCTGTTATTATTGTGAATGAAGAAGGAACAACAGCAGCAGCAGTTTCAGTT
GCATCAAGTTTTTCTATAAAACCAACTTTTACTGCAGATCGCCCTTTCTTGTATGCATTG
AGAACTAATTCAAATATTTATTTTTTGGGACTTTATGCTGGAAATCCACAATAA
>g15528.t1 Gene=g15528 Length=397
MECFKEFLIIYAAVFHSIHFVKTQDVGFLNAQQKFALELFSTISGPDHSTDVILSPISVS
TALSILLIGSNGVTFKQLFFGLQYPTNYSINLVQVNSNLMIKNMAKVGAIEFATKLFITK
SFSLQNNFKTSIDKYFQSGVESVDFSQNVKAAQRINDYISTSTHGMIQDVIKSDDLTEYT
TMILVNCIFFKGLWQFPFDKSYTFKSDFNIDNKNTIQVDYMYQENSFNFGYINALNDAQV
LELPYANSKLTLTLILPQTIDGIKNLVKAAKNFDFTKLSNQLTNTSVIVSIPKFNTTFEQ
YLNSPLTNMGMPLLFNKTVSQLDHIAHEFTVPKSLALDGLYHKAVIIVNEEGTTAAAVSV
ASSFSIKPTFTADRPFLYALRTNSNIYFLGLYAGNPQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g15528.t1 | CDD | cd00172 | serpin | 31 | 390 | 3.7663E-102 |
| 8 | g15528.t1 | Gene3D | G3DSA:3.30.497.10 | Antithrombin | 48 | 345 | 1.4E-90 |
| 7 | g15528.t1 | Gene3D | G3DSA:2.30.39.10 | - | 190 | 390 | 1.4E-90 |
| 2 | g15528.t1 | PANTHER | PTHR11461 | SERINE PROTEASE INHIBITOR, SERPIN | 30 | 393 | 6.3E-65 |
| 3 | g15528.t1 | PANTHER | PTHR11461:SF180 | LEUKOCYTE ELASTASE INHIBITOR | 30 | 393 | 6.3E-65 |
| 1 | g15528.t1 | Pfam | PF00079 | Serpin (serine protease inhibitor) | 31 | 390 | 4.2E-80 |
| 6 | g15528.t1 | ProSitePatterns | PS00284 | Serpins signature. | 370 | 380 | - |
| 5 | g15528.t1 | SMART | SM00093 | serpin2 | 37 | 396 | 2.2E-70 |
| 4 | g15528.t1 | SUPERFAMILY | SSF56574 | Serpins | 15 | 392 | 1.11E-87 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005615 | extracellular space | CC |
| GO:0004867 | serine-type endopeptidase inhibitor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13965 (serpin B8)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed