General information for g15556.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 9e2.
Parent gene
- The parent gene g15556 is located on chr_4: 5844898..5847211 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15556 | g15556.t2 | TSS | g15556.t2 | 5843654 | 5843654 |
| chr_4 | g15556 | g15556.t2 | isoform | g15556.t2 | 5843889 | 5845646 |
| chr_4 | g15556 | g15556.t2 | exon | g15556.t2.exon1 | 5843889 | 5844100 |
| chr_4 | g15556 | g15556.t2 | cds | g15556.t2.CDS1 | 5843889 | 5844100 |
| chr_4 | g15556 | g15556.t2 | exon | g15556.t2.exon2 | 5844213 | 5845646 |
| chr_4 | g15556 | g15556.t2 | cds | g15556.t2.CDS2 | 5844213 | 5845422 |
| chr_4 | g15556 | g15556.t2 | TTS | g15556.t2 | NA | NA |
Sequences
>g15556.t2 Gene=g15556 Length=1646
ATGAGTATCACATTGATTTTTATTTGGATTTTAATCTTTTTATTTTGGTTTGTTTATCGA
AAGTACAAAAAGAATTTCGATGAATTTGAAAGTTATGGTTTGAAATATATGAAGCCATGG
GAAGGAGTTGGAAGTTTTTTCAAAATTATGCTACTTAAAATAACTATGTTTGATGCTGTA
GAAGATTTTTATAGAAAATTTTCTGGCCAAAAAGTCGCTCCAGCATTGTCAATTCTTAAT
CAGCGAGTTTTCTTAATCCGTGATCCAACAATCATCAAGCAAATAGCAATAAAAGATTTC
GATCATTTCGTCAATCATGACAAAACAGTCGATGAAAATCTTGATCCAGTCATTGGAAAA
AATCTCTTCACATTACGTGATGAAAGTTGGCGGAAAATGCGAAATAGTCTTAGTCCTATT
TTTACAAGCAGTAAAATGAAAATGATGTTTGGAATTCTTTCAGAAACAGCCGACGATTTT
GTTGATTTTTGCAATGAAAAACTTGAAAGTGAAGGAAAAATTGAAATTGATTGTAAGAGC
ATTTTTGTACGTTTTGCAGTTGATGGAATAAGCACAGCAGTTTTAGGTTTCAAAGCAGAT
TGTTTAAGAAATGAAAAAAGTCAGGTTTATGAGATGGTTCAAAAAATTATCAAACCACCA
AGTTCTGGAATTTTTAAAATTTTAATTTTTGTCTTTGCCAAATGGCTCTACAAACTACTC
AAGTTAAAAGTTCTTCCACCAGATGTTTATGAATTTTTCAAAGCAGCAATTGTAAACGTC
ATGAAAGAGAGAGAAGAAAAAGGAATTTTTAGACCTGACGTAATCGAACTGTTAATGCGA
GCTAAACGTGGACAATTAGCAGCTGCAGATAAAAATAATGAAGTCAATGAAGCTGAGTTG
TCGAATTTTTCAGCAAATCCTGAATATGAAGTGAAATCGAAAAATTCAATTATTTGGACT
GATGATCATTTCATTGCACAAGGTCATCTATTTTTCTCTGCTGGTCTCGCAACTTCATCA
GTTGTTCTTGAAACAGTTGCTTACTATCTTGCAGTGAATAAAAATTATCAAAATGAAATA
ATCAAAGAAGTCGATGCAGTTGAAAATCCAACTTATGAAGCAATTCATAAAATGAAATTT
CTTGACATGTTTATCAGTGAAGTTTTGAGACTTTGGGCTTCACCATTTTTGAATAGAGAA
TGTAATAAGGATTATAAAATGGATTTTGGTGATGGAATTGTTATAAATGTAAAAAATGGC
GATTTTGTTCAATTTCCTGTTTGGTCAATTCATCATGATGAAAAATATTTTCCAAATCCA
GAAGTTTTTGATCCTTATCGATTTAGTGATGAAAGAAAAGGAGAAATTTTGAATTATTAT
ATGCCATTCGGTATTGGACCAAGAATTTGTATTGTCAGTTAAAAATTCAAAATACTGCAA
ATTTTTTTTTACAATTTTTTTATTCTTTTTAGGCTTCACGTTTTGCATTGATGGAAGTAA
AAGTGCTAATTTTTAAAATTTTATCAAAGTTTACTTTTGAAGCATGTGAAAAAACACCAA
AGAAATTGAAAGCAAAACCAAGTTTAACAGAATTTCAATTTACCGAAGATATTATTGTCG
AATTGAAACCAAGGGATGGAAAATAA
>g15556.t2 Gene=g15556 Length=473
MSITLIFIWILIFLFWFVYRKYKKNFDEFESYGLKYMKPWEGVGSFFKIMLLKITMFDAV
EDFYRKFSGQKVAPALSILNQRVFLIRDPTIIKQIAIKDFDHFVNHDKTVDENLDPVIGK
NLFTLRDESWRKMRNSLSPIFTSSKMKMMFGILSETADDFVDFCNEKLESEGKIEIDCKS
IFVRFAVDGISTAVLGFKADCLRNEKSQVYEMVQKIIKPPSSGIFKILIFVFAKWLYKLL
KLKVLPPDVYEFFKAAIVNVMKEREEKGIFRPDVIELLMRAKRGQLAAADKNNEVNEAEL
SNFSANPEYEVKSKNSIIWTDDHFIAQGHLFFSAGLATSSVVLETVAYYLAVNKNYQNEI
IKEVDAVENPTYEAIHKMKFLDMFISEVLRLWASPFLNRECNKDYKMDFGDGIVINVKNG
DFVQFPVWSIHHDEKYFPNPEVFDPYRFSDERKGEILNYYMPFGIGPRICIVS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g15556.t2 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 472 | 9.5E-88 |
| 2 | g15556.t2 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 471 | 3.6E-114 |
| 5 | g15556.t2 | PRINTS | PR00465 | E-class P450 group IV signature | 378 | 394 | 1.3E-9 |
| 3 | g15556.t2 | PRINTS | PR00465 | E-class P450 group IV signature | 431 | 449 | 1.3E-9 |
| 4 | g15556.t2 | PRINTS | PR00465 | E-class P450 group IV signature | 454 | 470 | 1.3E-9 |
| 1 | g15556.t2 | Pfam | PF00067 | Cytochrome P450 | 43 | 471 | 3.0E-57 |
| 10 | g15556.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 11 | g15556.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g15556.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 13 | g15556.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 9 | g15556.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 473 | - |
| 7 | g15556.t2 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 38 | 471 | 1.44E-73 |
| 6 | g15556.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
