General information for g15750.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Adenylosuccinate lyase.
Parent gene
- The parent gene g15750 is located on chr_4: 6651902..6652768 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15750 | g15750.t1 | TSS | g15750.t1 | 6651125 | 6651125 |
| chr_4 | g15750 | g15750.t1 | isoform | g15750.t1 | 6651215 | 6652703 |
| chr_4 | g15750 | g15750.t1 | exon | g15750.t1.exon1 | 6651215 | 6651346 |
| chr_4 | g15750 | g15750.t1 | cds | g15750.t1.CDS1 | 6651215 | 6651346 |
| chr_4 | g15750 | g15750.t1 | exon | g15750.t1.exon2 | 6651399 | 6652703 |
| chr_4 | g15750 | g15750.t1 | cds | g15750.t1.CDS2 | 6651399 | 6652703 |
| chr_4 | g15750 | g15750.t1 | TTS | g15750.t1 | 6652762 | 6652762 |
Sequences
>g15750.t1 Gene=g15750 Length=1437
ATGGATCATACAGGCTATCGCTCACCCTTATCAACACGTTATGCTTCAAAAGAAATGCAA
TATTTGTTTTCTGATCAATTCAAGTTTTCAACATGGAGAAAATTATGGATTATGCTTGCT
AAAGCTGAAATGGAACTTGGTCTTCCAATAACTCAAGCACAAATTCAAGAAATGGAAGCT
CATGTAGATGATGTCGATTTTGCAGCAGCAGCTCAAGAAGAAAAAGCAACTCGTCATGAT
GTCATGGCTCATGTTCATGTTTTTGCTTCACAATGTCCAACTGCAGCTCCAATTATTCAT
TTGGGAGCAACTTCATGCTTTGTTGGTGACAATACTGATCTGATTATTCTTCGTGATGCT
CTTGATTTGCTTCTTTTACGTGTTGCAAGTGTCATCAAATCACTTATGGATTTTGCACTC
AAATATAAAGATCTTCCAACTCTTGGTTTCACTCATTTACAACCAGCACAATTAACAACA
GTTGGTAAACGCTGTACATTGTGGGTTCAAGATTTGGTCATGGATGAAAGAAATTTACGA
TTTTTGCGTGAAAATTTAAGATTTCGCAGTACAAAAGGAACAACTGGAACTCAAGCAAGT
TTTCTTGAATTGTTCAATGGTGATAGTGCTAAAGTTAAACAACTTGATGCAAAAGTAACT
GAACTTGCTGGATTTAGCAAGAGTTATGCTGTTACAGGACAAACTTACACTCGAAAAGTT
GATGTCGATGTTGTATCAGCAATTGCAAGTCTTGGATCTTCTGTTCATAAAATGTGCAGT
GATTTACGCTTGTTGGCATCAATGAAGGAAGTTGAGGAGCCTTTTGAGACAACACAAATT
GGATCATCAGCAATGCCTTATAAACGTAATCCAATGCGTTCTGAAAGATGTTGTGCACTT
GCCCGTCATTTGATTACGCTTCAATCAAATGCAGCCAACACTCATGCAACACAATGGATG
GAACGAACACTTGATGATTCTGCAAATCGTAGATTGACACTTTCTGAAAGTTTTCTTGCT
GCCGATGCGTGCCTTTTGACACTTTTGAATATTTCTCAAGGAATGGTTGTTTATCCGAAA
GTTATTGAACGCCATATTTTGCAAGAATTGCCATTTATGGCTGCTGAAAATGTCATTATG
GCAATGGTCAAAGCTGGTGGTGATCGTCAAGTGTGCCATGAGAAAATTCGAGTTCTATCA
CATGAAGCTGGTGCACAAGTTAAGCAACATGGAAAAGACAACGACTTAGTTGAAAGAATC
AAAGCTGACAAATATTTTGAGCCAATTTTGGGACAACTTGAGCACATTTTAGATGCAAAA
ACTTTCACTGGAAGAGCTGGAGAACAAGTTGTTGAATTTTATGAGGAAGAAGTCAAAGAA
ATTTTGGAAAAATATGCTGACAAAATTCAAACTAAAGAAGTTAAATTGGAAATTTAA
>g15750.t1 Gene=g15750 Length=478
MDHTGYRSPLSTRYASKEMQYLFSDQFKFSTWRKLWIMLAKAEMELGLPITQAQIQEMEA
HVDDVDFAAAAQEEKATRHDVMAHVHVFASQCPTAAPIIHLGATSCFVGDNTDLIILRDA
LDLLLLRVASVIKSLMDFALKYKDLPTLGFTHLQPAQLTTVGKRCTLWVQDLVMDERNLR
FLRENLRFRSTKGTTGTQASFLELFNGDSAKVKQLDAKVTELAGFSKSYAVTGQTYTRKV
DVDVVSAIASLGSSVHKMCSDLRLLASMKEVEEPFETTQIGSSAMPYKRNPMRSERCCAL
ARHLITLQSNAANTHATQWMERTLDDSANRRLTLSESFLAADACLLTLLNISQGMVVYPK
VIERHILQELPFMAAENVIMAMVKAGGDRQVCHEKIRVLSHEAGAQVKQHGKDNDLVERI
KADKYFEPILGQLEHILDAKTFTGRAGEQVVEFYEEEVKEILEKYADKIQTKEVKLEI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15750.t1 | CDD | cd03302 | Adenylsuccinate_lyase_2 | 10 | 445 | 0.0 |
| 14 | g15750.t1 | Coils | Coil | Coil | 451 | 471 | - |
| 11 | g15750.t1 | Gene3D | G3DSA:1.10.275.10 | - | 3 | 101 | 3.4E-40 |
| 13 | g15750.t1 | Gene3D | G3DSA:1.20.200.10 | Fumarase/aspartase (Central domain) | 102 | 414 | 1.8E-121 |
| 12 | g15750.t1 | Gene3D | G3DSA:1.20.200.10 | Fumarase/aspartase (Central domain) | 415 | 474 | 2.4E-7 |
| 3 | g15750.t1 | PANTHER | PTHR43172 | ADENYLOSUCCINATE LYASE | 4 | 468 | 4.2E-202 |
| 5 | g15750.t1 | PRINTS | PR00145 | Argininosuccinate lyase family signature | 139 | 159 | 5.3E-5 |
| 7 | g15750.t1 | PRINTS | PR00149 | Fumarate lyase superfamily signature | 144 | 162 | 1.2E-12 |
| 4 | g15750.t1 | PRINTS | PR00145 | Argininosuccinate lyase family signature | 238 | 262 | 5.3E-5 |
| 9 | g15750.t1 | PRINTS | PR00149 | Fumarate lyase superfamily signature | 238 | 265 | 1.2E-12 |
| 6 | g15750.t1 | PRINTS | PR00145 | Argininosuccinate lyase family signature | 281 | 297 | 5.3E-5 |
| 8 | g15750.t1 | PRINTS | PR00149 | Fumarate lyase superfamily signature | 281 | 297 | 1.2E-12 |
| 1 | g15750.t1 | Pfam | PF00206 | Lyase | 33 | 297 | 1.7E-27 |
| 2 | g15750.t1 | Pfam | PF10397 | Adenylosuccinate lyase C-terminus | 371 | 451 | 7.4E-16 |
| 16 | g15750.t1 | ProSitePatterns | PS00163 | Fumarate lyases signature. | 281 | 290 | - |
| 17 | g15750.t1 | SMART | SM00998 | ADSL_C_2 | 370 | 454 | 1.4E-23 |
| 10 | g15750.t1 | SUPERFAMILY | SSF48557 | L-aspartase-like | 8 | 454 | 9.11E-100 |
| 18 | g15750.t1 | TIGRFAM | TIGR00928 | purB: adenylosuccinate lyase | 10 | 452 | 1.1E-120 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004018 | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity | MF |
| GO:0009152 | purine ribonucleotide biosynthetic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01756 (adenylosuccinate lyase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
