General information for g15785.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lactoylglutathione lyase.
Parent gene
- The parent gene g15785 is located on chr_4: 6775310..6776119 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15785 | g15785.t5 | isoform | g15785.t5 | 6774847 | 6775930 |
| chr_4 | g15785 | g15785.t5 | exon | g15785.t5.exon1 | 6774847 | 6775309 |
| chr_4 | g15785 | g15785.t5 | cds | g15785.t5.CDS1 | 6775049 | 6775309 |
| chr_4 | g15785 | g15785.t5 | exon | g15785.t5.exon2 | 6775368 | 6775645 |
| chr_4 | g15785 | g15785.t5 | cds | g15785.t5.CDS2 | 6775368 | 6775634 |
| chr_4 | g15785 | g15785.t5 | exon | g15785.t5.exon3 | 6775929 | 6775930 |
| chr_4 | g15785 | g15785.t5 | TSS | g15785.t5 | 6776004 | 6776004 |
| chr_4 | g15785 | g15785.t5 | TTS | g15785.t5 | NA | NA |
Sequences
>g15785.t5 Gene=g15785 Length=743
CGAAACATAAAAAATGTCAGGCTTAACGGATGCTGAAGCAAATGCACTTTGCCGTCCATT
TGATGGCTCAACTAAGGATTTTCTCTTCCAACAAACAATGTATCGCATCAAAGATCCAAG
AAAAACACTTCCATTTTACTCTGAAGTGCTTGGCATGACACTTTTAGTCAAATTGGATTT
TCCTGAAGCTAAATTTTCTCTCTATTTTATGGGATATGAAAATCCTGCTGATGTTCCAAG
TGATCCAGCTAAAAGAAAAGTTTGGGCAATGTCAAGAAAGGCAACTGTTGAATTGACTCA
CAATTGGGGAACTGAAGATGATCCAAATCAAAGTTATCATAATGGCAATAATGATCCACG
TGGATTTGGACACATTGGCATTATGGTTCCAAATGTTGAAGAAGCTTGTGCACGATTTGA
GAAATTTGGTGTTGAATTTGTTAAGAAACCAAACGATGGTCGCATGAAAGGACTTGCTTT
TATTAAAGATCCAGATGGCTACTGGATTGAAATTTTCAATGCTGGAACTGTGCCATGCTG
AAAACATTTCATGTAAAAATGTGAAACTTTTAAGTATCCACATCTGTTGATTTCTATTTT
TAGACAAACAACACTGAAAACATATTTGAGAAAAAATAAACAAATCAAAGAAAATATTGG
GAAAATTGATTTTTCTTTACTTCGGCGCTATTGAATTTTCATTAAAAGATTTACTTTTTC
TTTAATATTTAAATTTATTTTAT
>g15785.t5 Gene=g15785 Length=175
MSGLTDAEANALCRPFDGSTKDFLFQQTMYRIKDPRKTLPFYSEVLGMTLLVKLDFPEAK
FSLYFMGYENPADVPSDPAKRKVWAMSRKATVELTHNWGTEDDPNQSYHNGNNDPRGFGH
IGIMVPNVEEACARFEKFGVEFVKKPNDGRMKGLAFIKDPDGYWIEIFNAGTVPC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g15785.t5 | CDD | cd07233 | GlxI_Zn | 25 | 168 | 4.03492E-97 |
| 5 | g15785.t5 | Gene3D | G3DSA:3.10.180.10 | 2 | 1 | 172 | 6.9E-67 |
| 2 | g15785.t5 | PANTHER | PTHR10374:SF30 | LACTOYLGLUTATHIONE LYASE | 12 | 172 | 1.6E-80 |
| 3 | g15785.t5 | PANTHER | PTHR10374 | LACTOYLGLUTATHIONE LYASE GLYOXALASE I | 12 | 172 | 1.6E-80 |
| 1 | g15785.t5 | Pfam | PF00903 | Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily | 27 | 167 | 4.2E-19 |
| 7 | g15785.t5 | ProSitePatterns | PS00934 | Glyoxalase I signature 1. | 27 | 48 | - |
| 8 | g15785.t5 | ProSiteProfiles | PS51819 | Vicinal oxygen chelate (VOC) domain profile. | 24 | 170 | 24.857 |
| 4 | g15785.t5 | SUPERFAMILY | SSF54593 | Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase | 24 | 170 | 2.89E-34 |
| 9 | g15785.t5 | TIGRFAM | TIGR00068 | glyox_I: lactoylglutathione lyase | 16 | 172 | 5.1E-56 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004462 | lactoylglutathione lyase activity | MF |
| GO:0046872 | metal ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K01759 (lactoylglutathione lyase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
