General information for g15817.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6a2.
Parent gene
- The parent gene g15817 is located on chr_4: 6869203..6871897 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15817 | g15817.t1 | isoform | g15817.t1 | 6867472 | 6871897 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon1 | 6867472 | 6867638 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS1 | 6867472 | 6867638 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon2 | 6867826 | 6868077 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS2 | 6867826 | 6868077 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon3 | 6868258 | 6868394 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS3 | 6868258 | 6868394 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon4 | 6868537 | 6868936 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS4 | 6868537 | 6868936 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon5 | 6869191 | 6869724 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS5 | 6869191 | 6869724 |
| chr_4 | g15817 | g15817.t1 | TTS | g15817.t1 | 6870279 | 6870279 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon6 | 6870435 | 6870601 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS6 | 6870435 | 6870601 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon7 | 6870778 | 6870836 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS7 | 6870778 | 6870836 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon8 | 6870998 | 6871134 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS8 | 6870998 | 6871134 |
| chr_4 | g15817 | g15817.t1 | exon | g15817.t1.exon9 | 6871837 | 6871897 |
| chr_4 | g15817 | g15817.t1 | cds | g15817.t1.CDS9 | 6871837 | 6871897 |
| chr_4 | g15817 | g15817.t1 | TSS | g15817.t1 | 6871946 | 6871946 |
Sequences
>g15817.t1 Gene=g15817 Length=1914
ATGAAAATTGTGACAAAATATATAGTTTTAGGATTAATATTCACAGGAAAATTTGTTGAA
GCTCTTCGTTGTTATTCTTGTTCAATAACATCACAAAACACAGACATGAAATGTTTGCAT
GATCCTGTTAGTGTTGAAGGCCAAAGTGTAGTAAATTGCAATAAAAAATATTGTACAATT
TTAAGACAAGAACTACAGGATCCAGCTGGTAAAATTAATACTTTCGTTCGTGGGTGTGAA
GAAAAACCAATTTTACTAAATGACGTAATCGATGATCCAATGTTCCGAACTTATTATCGA
TCATGTTCAGAAGATCTCTGCAATGCTGGAGATGCTATAAAAAATCCATCAGATCAAAAA
ATATCACCTGATGGTTACGATGGTGAAAATTTACTAGTTGCTGGTTTACCATTTAATAGT
AGTTCAATTTCGTTTGTATTGATTTTTTGGTTTGTTAAAAATCGTTTTAATTATTGGAAA
AATCAAAATGTTCCTTTTCTTCAACCAAAATTTCCCTATGGAAATATCAAAGGATTTGGT
AAAAACTTTCATCAATTTGAATTTCAACAAAAAATCTACGAAATTTATAAGAAGAAACTT
TCGCCATTTGTTGGTCTTTATTATTACATTCAACCAGTTGTTTTATGCACAGACTTGGAA
TTTATCAAAAATATTTTCATGCGTGATTCTGCTTATTTTCTTGATCGTGGCGCTTATTAC
AATGAAAAAGACAATCCAATTTCAGCACATCTATTTAATCTTGACAATCCAAAATGGAAA
ATATTAAGGAACAAATTAACGCCAACTTTTACATCAGGAAAGATCAAGACAATGTTTTTT
ACTCTTTGTGATGTTGGTGAAAGATTGGTGAAAAAATTAGAAAAAGAAGTAGAAATTGCA
GATGATGGAATTTTAGAAATGAAGGAATTTTCTGCCAGATTTACAACTGATGTTATTGGT
TCATTTGGATTTGGGATTGAAATAAATTCCCTTGAGCATCCTGATACTGAATTTAGAGTT
CGTGGAAGTCAGGTTTTTGAAAAGCCAAAATATCCACCAATATTTTTTCAATTTTGTGTG
AATTTCAAACCTTTAGCACGAATGCTTAAAATTAGCACCACTGCTAAAGAAATTGAGGAA
TTTTTTACAAGAATTGTAAAAGAAACAATTGAATATCGTGAAAAAAATCAAATAATTCGA
AATGATTTTATGAATTTGTTGATACAATTGAAAAATTTTGGGAAACTTGAAGGAGACATA
ACAAGTATAGGAAAATTGACATTAAATGAAATTGTTGCTCAATGTTTTATCTTTTTTGCT
GCTGGATATGAAACAAGTTCAACTACAATGGCTTATGCTTTATTTGAGTTAGCATTGAAC
CAAAATATTCAAGATAAGGCGAGAGATAATGTAAGAAAAATATTGAGCAAATATGATGGA
AAGTTTACATATGAAGGTCTTATGGAAATGGATTATGTTGACATGTGTGTTAATGAAACT
CTCAGAAAATATCCTCCAGTATCATTCATCACACGATATTGTAGTAATGATTACAAAATA
GAACACACAGAACATGTTATCAAAAAAGGTCAACTTGTCATTATTCCAACTTATGCCATT
CATCATGATAAAGATATTTTCCCTAATCCAGAAGTTTTTGATCCTGAAAGATTTTCACAA
TTGGAATCACCAAAAATACCGCAAGTTGCTTTTATGCCTTTTGGACATGGTGCTAGGAAT
TGTATAGGACTTCGCTTTGGACAAATTCAAGCAAAAGTTGGACTGGCAATGTTGCTAAAT
AATTTCAAATTTGAGCTGTCGGACAAGACTCAAAATCCATTGAAATTTAGAAAAGATACA
ATAGTTATGCTTATTGCTGAAGGTGGAATTCATTTGAAAGTTACCAAAATATAA
>g15817.t1 Gene=g15817 Length=637
MKIVTKYIVLGLIFTGKFVEALRCYSCSITSQNTDMKCLHDPVSVEGQSVVNCNKKYCTI
LRQELQDPAGKINTFVRGCEEKPILLNDVIDDPMFRTYYRSCSEDLCNAGDAIKNPSDQK
ISPDGYDGENLLVAGLPFNSSSISFVLIFWFVKNRFNYWKNQNVPFLQPKFPYGNIKGFG
KNFHQFEFQQKIYEIYKKKLSPFVGLYYYIQPVVLCTDLEFIKNIFMRDSAYFLDRGAYY
NEKDNPISAHLFNLDNPKWKILRNKLTPTFTSGKIKTMFFTLCDVGERLVKKLEKEVEIA
DDGILEMKEFSARFTTDVIGSFGFGIEINSLEHPDTEFRVRGSQVFEKPKYPPIFFQFCV
NFKPLARMLKISTTAKEIEEFFTRIVKETIEYREKNQIIRNDFMNLLIQLKNFGKLEGDI
TSIGKLTLNEIVAQCFIFFAAGYETSSTTMAYALFELALNQNIQDKARDNVRKILSKYDG
KFTYEGLMEMDYVDMCVNETLRKYPPVSFITRYCSNDYKIEHTEHVIKKGQLVIIPTYAI
HHDKDIFPNPEVFDPERFSQLESPKIPQVAFMPFGHGARNCIGLRFGQIQAKVGLAMLLN
NFKFELSDKTQNPLKFRKDTIVMLIAEGGIHLKVTKI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g15817.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 147 | 637 | 2.1E-131 |
| 2 | g15817.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 145 | 637 | 1.4E-183 |
| 3 | g15817.t1 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 145 | 637 | 1.4E-183 |
| 7 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 197 | 216 | 3.3E-18 |
| 9 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 430 | 447 | 3.3E-18 |
| 11 | g15817.t1 | PRINTS | PR00385 | P450 superfamily signature | 441 | 458 | 3.3E-11 |
| 5 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 450 | 476 | 3.3E-18 |
| 10 | g15817.t1 | PRINTS | PR00385 | P450 superfamily signature | 495 | 506 | 3.3E-11 |
| 4 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 536 | 560 | 3.3E-18 |
| 6 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 571 | 581 | 3.3E-18 |
| 12 | g15817.t1 | PRINTS | PR00385 | P450 superfamily signature | 572 | 581 | 3.3E-11 |
| 8 | g15817.t1 | PRINTS | PR00463 | E-class P450 group I signature | 581 | 604 | 3.3E-18 |
| 13 | g15817.t1 | PRINTS | PR00385 | P450 superfamily signature | 581 | 592 | 3.3E-11 |
| 1 | g15817.t1 | Pfam | PF00067 | Cytochrome P450 | 180 | 624 | 2.3E-98 |
| 18 | g15817.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 19 | g15817.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 20 | g15817.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 16 | - |
| 21 | g15817.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 17 | g15817.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 637 | - |
| 22 | g15817.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 574 | 583 | - |
| 14 | g15817.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 174 | 635 | 4.45E-110 |
| 15 | g15817.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K14999 (cytochrome P450 family 6 [EC:1.14.-.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed