General information for g15841.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as E3 SUMO-protein ligase PIAS3.
Parent gene
- The parent gene g15841 is located on chr_4: 6989741..6991957 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15841 | g15841.t3 | TTS | g15841.t3 | 6989475 | 6989475 |
| chr_4 | g15841 | g15841.t3 | isoform | g15841.t3 | 6989741 | 6991957 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon1 | 6989741 | 6990309 |
| chr_4 | g15841 | g15841.t3 | cds | g15841.t3.CDS1 | 6989741 | 6990309 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon2 | 6990448 | 6990525 |
| chr_4 | g15841 | g15841.t3 | cds | g15841.t3.CDS2 | 6990448 | 6990525 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon3 | 6990757 | 6990856 |
| chr_4 | g15841 | g15841.t3 | cds | g15841.t3.CDS3 | 6990757 | 6990856 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon4 | 6990913 | 6991257 |
| chr_4 | g15841 | g15841.t3 | cds | g15841.t3.CDS4 | 6990913 | 6991257 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon5 | 6991320 | 6991398 |
| chr_4 | g15841 | g15841.t3 | cds | g15841.t3.CDS5 | 6991320 | 6991352 |
| chr_4 | g15841 | g15841.t3 | exon | g15841.t3.exon6 | 6991660 | 6991957 |
| chr_4 | g15841 | g15841.t3 | TSS | g15841.t3 | 6992076 | 6992076 |
Sequences
>g15841.t3 Gene=g15841 Length=1469
ATGAAAAGAACGAGGCAATCAGATAAACTAACAGACGACGAAAGTTTTAAAGTAAGAAAA
AATTTTTTTTCTGATCATGAAAGAAGTTTTTAATTTTTATTCTCTTGAAATTGTAGAAAA
AAGTTGGTTTTCTTCGAGTCATTGATTTGCATTCAATTCTTGCTGTTTTTAAATATCCGA
AAAATTCACGAAAGGATGCATTACAACGTAGATTATTGGAATTATTAAAAGGAAGGGAAA
TTAGTGAAGCACAAAAATTGGAAATAAGAAAACAAGTCGAAAAAACTTTTGATAAAATTC
AATCACTCGCTCCTAGACGATCAAAAACGCCAGATAGAACAGATATGTATGCTCAACAAT
TTAGCACTAATCCACTTGCACCGCATAATGTCACACCATTTGTATCATCAGGGTTTCCAA
TTCAACCTGATGTGAAACTCAAAAAACTCGCCTTTTACGATGTTCTTGGAACTTTACTTC
AACCTTCAACTTTAATGCCAGCAAGTCAAGCTAGACAACAAGAAGGAACTTATTATTTTC
ATTTGACACCACAACAAGCTACTGATATTGCTATGAATCGAGATATCAGAAACCCAGCTA
AACCAGAATATCTCATTCAAGTTCAATTAAGATTTTGCTTACTTGAAACAACGTCAGAAC
AAGAAGATTGTTTTCCGCCAAATGTTCAAGTTAAAGTTAATGGAAAACTGTGTCAGTTGC
CAAATCCAATTGCAACAAACAAACCAGGAGTAGAACCAAGAAGACCACCACGACCAGTAA
ACATCACACCACATGTGAAAATTTCACCAACTGTTGCTAACGTAATTCATGTTCAATGGC
AGACAGAATTTAACAGAGCTTTCGTTATTTCTTGCTATTTAGTAAGGAAATTAACATCAG
ATTGTTTACTTCAAAGAATGAAAACGAAAGGAAGCAAACCATCAGAATATACAAGAGGAT
TAATTAAAGAAAAGCTAAATGAAGATGCAGATTGTGAAATTGCAACAACAATGCTTCGAG
TCTCATTAATTTGTCCACTTGGTAAAATGAGAATGACAACACCATGTCGGTCTTCATTAT
GCATGCATTTACAATGCTTTGATGCATCTCTCTATTTACAAATGAATGAAAGAAAGCCAA
CTTGGAACTGTCCAGTTTGTGACAAACAGGCACCATATGAAACATTGGTAATTGATGGAT
ATTTCCAAGAAGTTATTGGATCACAAAAGTTAGGACTTGATGATAATGAAATTCAATTGC
ATAAAGATGGCGAATGGAGTACATTTGATACAAAAGCTGAAAAAGAAATTGCAACGAAAA
GTTTGACAAAAGTCGAAATTATTTCTGATGATCTTGAAGTTGTTTCAGAAGATTTGCCAA
AGTCAAAAACAGGTGCAATTGGAAATGGAACTGCAAAACCTAATGCCGAGACTGTTGATT
TGACACTTGATAGTGATGAAGAGAAATAG
>g15841.t3 Gene=g15841 Length=374
MYAQQFSTNPLAPHNVTPFVSSGFPIQPDVKLKKLAFYDVLGTLLQPSTLMPASQARQQE
GTYYFHLTPQQATDIAMNRDIRNPAKPEYLIQVQLRFCLLETTSEQEDCFPPNVQVKVNG
KLCQLPNPIATNKPGVEPRRPPRPVNITPHVKISPTVANVIHVQWQTEFNRAFVISCYLV
RKLTSDCLLQRMKTKGSKPSEYTRGLIKEKLNEDADCEIATTMLRVSLICPLGKMRMTTP
CRSSLCMHLQCFDASLYLQMNERKPTWNCPVCDKQAPYETLVIDGYFQEVIGSQKLGLDD
NEIQLHKDGEWSTFDTKAEKEIATKSLTKVEIISDDLEVVSEDLPKSKTGAIGNGTAKPN
AETVDLTLDSDEEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g15841.t3 | CDD | cd16790 | SP-RING_PIAS | 227 | 274 | 1.25458E-30 |
| 6 | g15841.t3 | Gene3D | G3DSA:2.60.120.780 | - | 29 | 182 | 2.9E-58 |
| 7 | g15841.t3 | Gene3D | G3DSA:3.30.40.10 | Zinc/RING finger domain | 183 | 313 | 3.4E-41 |
| 5 | g15841.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 349 | 374 | - |
| 3 | g15841.t3 | PANTHER | PTHR10782 | ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN | 20 | 327 | 5.6E-110 |
| 4 | g15841.t3 | PANTHER | PTHR10782:SF11 | E3 SUMO-PROTEIN LIGASE PIAS1 | 20 | 327 | 5.6E-110 |
| 2 | g15841.t3 | Pfam | PF14324 | PINIT domain | 30 | 181 | 5.2E-31 |
| 1 | g15841.t3 | Pfam | PF02891 | MIZ/SP-RING zinc finger | 226 | 274 | 3.1E-22 |
| 9 | g15841.t3 | ProSiteProfiles | PS51466 | PINIT domain profile. | 16 | 183 | 49.354 |
| 8 | g15841.t3 | ProSiteProfiles | PS51044 | Zinc finger SP-RING-type profile. | 215 | 292 | 40.853 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008270 | zinc ion binding | MF |
| GO:0019789 | SUMO transferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04706 (E3 SUMO-protein ligase PIAS1 [EC:2.3.2.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
