General information for g15881.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Trypsin-3.
Parent gene
- The parent gene g15881 is located on chr_4: 7160689..7162412 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15881 | g15881.t1 | TSS | g15881.t1 | 7160590 | 7160590 |
| chr_4 | g15881 | g15881.t1 | isoform | g15881.t1 | 7160689 | 7162412 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon1 | 7160689 | 7160932 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS1 | 7160689 | 7160932 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon2 | 7160987 | 7161242 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS2 | 7160987 | 7161242 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon3 | 7161300 | 7161470 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS3 | 7161300 | 7161470 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon4 | 7161548 | 7161673 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS4 | 7161548 | 7161673 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon5 | 7161787 | 7161797 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS5 | 7161787 | 7161797 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon6 | 7161867 | 7161883 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS6 | 7161867 | 7161883 |
| chr_4 | g15881 | g15881.t1 | exon | g15881.t1.exon7 | 7162338 | 7162412 |
| chr_4 | g15881 | g15881.t1 | cds | g15881.t1.CDS7 | 7162338 | 7162412 |
| chr_4 | g15881 | g15881.t1 | TTS | g15881.t1 | 7162477 | 7162477 |
Sequences
>g15881.t1 Gene=g15881 Length=900
ATGAAAGTTTTCATCATTTTTTCAATTCTCTTTGCATCAACTTTGGCATTTCCTTATGAA
AGTTCAATAAATGAAAATCCTCTGTTGATGTATAACTCAATTCGATTGCCAATGGAACCC
GAATCAAGAATTGTTGGTGGTGATCAACATGACATTTACAAATATCCTTACATTTGCTCT
CTTCAATGGAATGGCTCACATCGTTGTGGTGCAAATATTATTAGTCACAGATTTTTAGTG
ACAGCTGCTCACTGCACAATTGGTCTCACTCCAACTCTTTTAACTGTTCGTTGCGGATCA
TCACGTTTTGATAGTGGTGGAAGAGTTTATAGAATTTCAACTATAATTAATCATCCAGAT
TATAATTCAGCAACAATTGACTATGATTTTGCTGTCATTGAAACTGAGGAAGAAATTGAA
TTTAACGAAGCAACTCAACCTATTAGACTTGCTGATCAAGATGAAGAATTACCACATGGA
CATTGCATGAAAACACTTGGCTGGGGAAATACAATGGATCCAAGTCAAACACGCGACTAT
CTACGCATTGTTTATGTGCCAAAAGTTTTTCTTGAAGAGTGTAGAAGATTGTATAGCACT
ATTAACGCAGCTGTAACTGAAAGAATGATTTGTGCTGGATTTAGTGAAGGTGGTCATGAT
GCATGCCAAGGGGATTCTGGTGGCCCTCTGGCTGATGGCAGTTATGATGATGTACGATGC
AGATTTCATGGTAAAAGATTTGCAGCACAACCAAGAGCAGCTGAAGGAAGACTTGAAAAA
CTTTATGGAATCGTTAGTTGGGGTTTTGGTTGCGCTAGACCAAATTTCCCTGGAGTCTAT
TCAAGAGTGGCTTCAGTTAGTAATTGGATTAAAAGACACGCTCATATTAAAGATGACTAA
>g15881.t1 Gene=g15881 Length=299
MKVFIIFSILFASTLAFPYESSINENPLLMYNSIRLPMEPESRIVGGDQHDIYKYPYICS
LQWNGSHRCGANIISHRFLVTAAHCTIGLTPTLLTVRCGSSRFDSGGRVYRISTIINHPD
YNSATIDYDFAVIETEEEIEFNEATQPIRLADQDEELPHGHCMKTLGWGNTMDPSQTRDY
LRIVYVPKVFLEECRRLYSTINAAVTERMICAGFSEGGHDACQGDSGGPLADGSYDDVRC
RFHGKRFAAQPRAAEGRLEKLYGIVSWGFGCARPNFPGVYSRVASVSNWIKRHAHIKDD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15881.t1 | CDD | cd00190 | Tryp_SPc | 44 | 293 | 4.79671E-81 |
| 9 | g15881.t1 | Gene3D | G3DSA:2.40.10.10 | - | 37 | 295 | 1.2E-64 |
| 2 | g15881.t1 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 29 | 294 | 1.8E-79 |
| 3 | g15881.t1 | PANTHER | PTHR24276:SF83 | GH13245P2-RELATED | 29 | 294 | 1.8E-79 |
| 6 | g15881.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 70 | 85 | 1.1E-10 |
| 5 | g15881.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 125 | 139 | 1.1E-10 |
| 4 | g15881.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 219 | 231 | 1.1E-10 |
| 1 | g15881.t1 | Pfam | PF00089 | Trypsin | 44 | 290 | 4.9E-55 |
| 11 | g15881.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g15881.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 13 | g15881.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 14 | g15881.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 10 | g15881.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 299 | - |
| 18 | g15881.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 80 | 85 | - |
| 19 | g15881.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 220 | 231 | - |
| 20 | g15881.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 44 | 295 | 31.084 |
| 17 | g15881.t1 | SMART | SM00020 | trypsin_2 | 43 | 290 | 5.9E-74 |
| 7 | g15881.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 5 | 293 | 4.99E-72 |
| 8 | g15881.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 16 | g15881.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed